Production and purification of xylitol by Scheffersomyces amazonenses via sugarcane hemicellulosic hydrolysate

Abstract

Xylitol was produced by Scheffersomyces amazonensis UFMG‐HMD‐26.3 in hemicellulosic hydrolysate obtained from a mixture of sugarcane bagasse and straw (1:1) in batch fermentation, and was recovered using supercritical fluid. The xylitol produced was 20.11 g L−1 and the extraction approach reached a xylitol recovery of 40.51% at a high purity level of 99.59%. An analysis of energy consumption for the xylitol production process showed that xylitol production from sugarcane biomass hemicellulosic hydrolysate is feasible, highlighting the potential use of this biomass in sustainable xylitol production and supercritical fluids as a robust proposal for xylitol separation. © 2020 Society of Chemical Industry and John Wiley & Sons, Ltd

Biological and Pharmacological Potential of Xylitol: A Molecular Insight of Unique Metabolism

“…Thus, the microbes with active xylose reductase pathway are possible biocatalysts for xylitol production at a large scale. Although "pathway B" predominates in fungi, e.g., Aspergillus niger [15], yeast, e.g., Candida guilliermondii [16], Pichia spitis [17], Pichia caribbica [18] and Scheffersomyces amazonensis [19], and dairy yeast, e.g., Kluyveromyces marxianus [20], but few bacteria, e.g., Enterobacter sp. [21], Corynebacterium, Enterobacter [22], Bacillus and Pseudomonas [23], were also reported for xylitol production (Table 3).…”

Novel potential yeast strains for the biotechnological production of xylitol from sugarcane bagasse

“…A low carbon to nitrogen ratio favors the generation of cell biomass, but a high ratio facilitates the formation of metabolites. Yeasts can digest different nitrogen sources, such as peptone, yeast extracts, ammonium sulfate ([NH 4 ] 2 SO 4 ), and urea, all of which have been extensively studied for xylitol production 19 . Another source of nutrients that has been investigated for xylitol production is rice bran, which is considered to be a rich source of proteins, iron (Fe), zinc (Zn), manganese (Mn), and vitamins B and E 20–22 .…”

“…Another source of nutrients that has been investigated for xylitol production is rice bran, which is considered to be a rich source of proteins, iron (Fe), zinc (Zn), manganese (Mn), and vitamins B and E 20–22 . Supplementation of sugarcane bagasse hemicellulosic hydrolysate with nutrients, such as rice bran, has been widely studied, including the formulation of culture media to produce xylitol using Meyerozyma guilliermondii (Candida guilliermondii ) 14,16,19 …”

“…Yeasts can digest different nitrogen sources, such as peptone, yeast extracts, ammonium sulfate ([NH 4 ] 2 SO 4 ), and urea, all of which have been extensively studied for xylitol production. 19 Another source of nutrients that has been investigated for xylitol production is rice bran, which is considered to be a rich source of proteins, iron (Fe), zinc (Zn), manganese (Mn), and vitamins B and E. [20][21][22] Supplementation of sugarcane bagasse hemicellulosic hydrolysate with nutrients, such as rice bran, has been widely studied, including the formulation of culture media to produce xylitol using Meyerozyma guilliermondii (Candida guilliermondii). 14,16,19 Candida tropicalis and Meyerozyma guilliermondii are yeasts commonly studied for the production of xylitol.…”

“…19 Another source of nutrients that has been investigated for xylitol production is rice bran, which is considered to be a rich source of proteins, iron (Fe), zinc (Zn), manganese (Mn), and vitamins B and E. [20][21][22] Supplementation of sugarcane bagasse hemicellulosic hydrolysate with nutrients, such as rice bran, has been widely studied, including the formulation of culture media to produce xylitol using Meyerozyma guilliermondii (Candida guilliermondii). 14,16,19 Candida tropicalis and Meyerozyma guilliermondii are yeasts commonly studied for the production of xylitol. 10,16 These species are regarded as opportunistic human pathogens, thus limiting their use in food and pharmaceutical production.…”