Production and recovery of poly(3‐hydroxybutyrate‐<i>co</i>‐3‐hydroxyvalerate) from biodiesel liquid waste (BLW)

Martla, Mananya; Umsakul, Kamontam; Sudesh, Kumar

doi:10.1002/jobm.201800279

Cited by 14 publications

(10 citation statements)

References 45 publications

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“…As a fact, PHBV still exhibits a high degree of crystallinity throughout a wide range of compositions from 0 to 95 mol% HV (Cai and Qiu, 2009). Different cultivation strategies have been designed for the incorporation of 3HV monomers in the forming polymeric chain, using organic acid precursors, such as propionic and valeric acid (Zinn et al, 2003;Masood et al, 2012;Follonier et al, 2014;Hilliou et al, 2016;Martla et al, 2018).…”

Section: Poly (3-hydroxybutyrate-co-hydroxyvalerate)mentioning

confidence: 99%

In vivo and Post-synthesis Strategies to Enhance the Properties of PHB-Based Materials: A Review

Turco

Santagata

Corrado

et al. 2021

Front. Bioeng. Biotechnol.

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The transition toward “green” alternatives to petroleum-based plastics is driven by the need for “drop-in” replacement materials able to combine characteristics of existing plastics with biodegradability and renewability features. Promising alternatives are the polyhydroxyalkanoates (PHAs), microbial biodegradable polyesters produced by a wide range of microorganisms as carbon, energy, and redox storage material, displaying properties very close to fossil-fuel-derived polyolefins. Among PHAs, polyhydroxybutyrate (PHB) is by far the most well-studied polymer. PHB is a thermoplastic polyester, with very narrow processability window, due to very low resistance to thermal degradation. Since the melting temperature of PHB is around 170–180°C, the processing temperature should be at least 180–190°C. The thermal degradation of PHB at these temperatures proceeds very quickly, causing a rapid decrease in its molecular weight. Moreover, due to its high crystallinity, PHB is stiff and brittle resulting in very poor mechanical properties with low extension at break, which limits its range of application. A further limit to the effective exploitation of these polymers is related to their production costs, which is mostly affected by the costs of the starting feedstocks. Since the first identification of PHB, researchers have faced these issues, and several strategies to improve the processability and reduce brittleness of this polymer have been developed. These approaches range from the in vivo synthesis of PHA copolymers, to the enhancement of post-synthesis PHB-based material performances, thus the addition of additives and plasticizers, acting on the crystallization process as well as on polymer glass transition temperature. In addition, reactive polymer blending with other bio-based polymers represents a versatile approach to modulate polymer properties while preserving its biodegradability. This review examines the state of the art of PHA processing, shedding light on the green and cost-effective tailored strategies aimed at modulating and optimizing polymer performances. Pioneering examples in this field will be examined, and prospects and challenges for their exploitation will be presented. Furthermore, since the establishment of a PHA-based industry passes through the designing of cost-competitive production processes, this review will inspect reported examples assessing this economic aspect, examining the most recent progresses toward process sustainability.

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Section: Poly (3-hydroxybutyrate-co-hydroxyvalerate)mentioning

confidence: 99%

In vivo and Post-synthesis Strategies to Enhance the Properties of PHB-Based Materials: A Review

Turco

Santagata

Corrado

et al. 2021

Front. Bioeng. Biotechnol.

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“…methane, glucose, fructose), linked to these precursors (Table 2). These studies have confirmed that methanotrophic bacteria, Ralstonia eutropha, Pseudomonas species and Hydrogenophaga pseudoflava were capable to produce only PHB when fed solely with the main substrate (Kim et al 1994;Inn et al 2003;Myung et al 2015;Cal et al 2016;Martla et al 2018) while the specie Ralstonia eutropha DSM 545 was capable to produce PH3HB4HB when fed with sole glycerol (Cavalheiro et al 2012). The addition of precursors led, to PHBV production in all cases investigated.…”

Section: Addition Of 3hv Fraction Precursorsmentioning

confidence: 76%

“…Recently, the archea Haloferax Mediterranei, an extremely halophilic microrganism, has gained a greater attention, due to its faster growth, its high PHBV productivity and its capacity of producing high quality products (Hou et al 2013). Different species of Bacillus, Methilobacterium, Pseudomonas and Rhodospirillacee have been also tested on various substrates (Ueda et al 1992;Smith et al 2008;Zuñiga et al 2013;Martla et al 2018;Balakrishna Pillai et al 2020). Finally, even though less studied if compared to the previous mentioned microorganisms, Alcaligenes, Comamonas, Halomonas and Rhodococcus have been found to be capable to produce PHBV (Choi et al, 2003;Kulkarni et al, 2010;Williams et al, 1994;Zakaria et al, 2013).…”

Section: Phbv Biosynthesis Processes Characteristics and Applicationsmentioning

confidence: 99%

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Improving biological production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) co-polymer: a critical review

Policastro

Pánico

Fabbricino

2021

Rev Environ Sci Biotechnol

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Although poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is the most promising biopolymer for petroleum-based plastics replacement, the low processes productivity as well as the high sale price represent a major barrier for its widespread usage. The present work examines comparatively the existing methods to enhance the yield of the PHBV co-polymer biologically produced and/or reduce their costs. The study is addressed to researchers working on the development of new biological production methods and/or the improvement of those currently used. At this aim, the authors have considered the analysis of some crucial aspects related to substrates and microorganism’s choice. The production strategies have been individuated, presented and discussed, either based on a single aspect (type of substrate or microorganism) or based on combined aspects (type of substrate and microorganism). Process operating conditions have been discussed as well. The analysis indicates that the addition of 3HV precursors is capable to dramatically enhance the hydroxyvalerate fraction in the produced biopolymers. On the other hand, due to the high costs of the 3HV precursors, the utilization of wild bacterial species capable to produce the hydroxyvalerate fraction from unrelated carbon sources (i.e. no 3HV precursors) also can be considered a valuable strategy for costs reduction. Moreover, metabolic engineering techniques can be successfully used to promote 3HV precursors-independent biosynthesis pathways and enhance the process productivity. The use of mixed cultures or extremophile bacteria avoids the need of sterile working conditions, and therefore favours the process scale-up. The utilization of the organic waste as substrate plays a key role for a sharp reduction of production costs. Finally, the selection of the most suitable substrate-microorganism combination cannot be separated by the adoption of an appropriate choice of reactor configuration and abiotic factors. Graphic abstract

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“…[ 181 ] The yield of PHB and PHBV using P. mendocina and mealworms were 55.83 ± 1.53 g L −1 with 88% purity and 37.00 ± 1.81 g L −1 with 89% purity, respectively. [ 183 ] Gram‐negative predator bacterium, Bdellovibrio bacteriovorus HD100, was used for PHA recovery where it attacks by penetrating the membrane and consuming its prey. [ 184 ] It has been reported that B. bacteriovorus HD100 encodes mcl‐ PHA depolymerase ( phaZ Bd ), decreasing the PHA recovery.…”

Section: Recovery Methods Involved In Pha (Downstream Processing)mentioning

confidence: 99%

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

Lhamo

Behera

Mahanty

2021

Biotechnology Journal

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Background Microbial polyhydroxyalkanoates (PHAs) produced using renewable resources could be the best alternative for conventional plastics. Despite their incredible potential, commercial production of PHAs remains very low. Nevertheless, sincere attempts have been made by researchers to improve the yield and economic viability of PHA production by utilizing low‐cost agricultural or industrial wastes. In this context, the use of efficient microbial culture or consortia, adoption of experimental design to trace ideal growth conditions, nutritional requirements, and intervention of metabolic engineering tools have gained significant attention. Purpose and scope This review has been structured to highlight the important microbial sources for PHA production, use of conventional and non‐conventional substrates, product optimization using experimental design, metabolic engineering strategies, and global players in the commercialization of PHA in the past two decades. The challenges about PHA recovery and analysis have also been discussed which possess indirect hurdle while expanding the horizon of PHA‐based bioplastics. Summary Selection of appropriate microorganism and substrate plays a vital role in improving the productivity and characteristics of PHAs. Experimental design‐based bioprocess, use of metabolic engineering tools, and optimal product recovery techniques are invaluable in this dimension. Conclusion Optimization strategies, which are being explored in isolation, need to be logically integrated for the successful commercialization of microbial PHAs.

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Production and recovery of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) from biodiesel liquid waste (BLW)

Cited by 14 publications

References 45 publications

In vivo and Post-synthesis Strategies to Enhance the Properties of PHB-Based Materials: A Review

In vivo and Post-synthesis Strategies to Enhance the Properties of PHB-Based Materials: A Review

Improving biological production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) co-polymer: a critical review

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

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