Production and regeneration of protoplasts from Grateloupia turuturu Yamada (Rhodophyta)

Abstract

Protoplasts were isolated enzymatically from the carrageenophyte red alga Grateloupia turuturu (Halymeniales, Rhodophyta) that occurs along the coast of the French Channel in Normandy. Effects of the main factors on the protoplast yield were identified to improve the isolation protocol. The optimal enzyme composition for cell wall digestion and protoplast viability consisted of 2% cellulase Onozuka R-10, 0.5% macerozyme R-10, 2% crude extract from viscera of Haliotis tuberculata, 0.8 M mannitol, 20 mM sodium citrate, 0.3% bovine serum albumin at 25°C, and 4-h incubation period. The protoplasts were approximately 5-15 μm in diameter, liberated mainly from the surface cell layers. Maximum yield was 1.5×10 7 protoplasts g -1 fresh tissue. The protoplasts underwent initial division after 14 days with a high density level of 1×10 6 cells mL -1 in culture medium and developed into microthalli of a line of two to six cells.

Effects of light-emitting diodes on protoplast regeneration from gametophytic cells of the commercial kelp <italic>Undaria pinnatifida</italic> (Laminariales, Phaeophyceae)

“…In seaweeds, protoplast regeneration has been accomplished in 40 species (Matsumura et al 2001, Reddy et al 2008, Yeong et al 2008, Lafontaine et al 2011, Wang et al 2014, Huddy et al 2015, Avila-Peltroche et al 2019. Although different factors affecting protoplast regeneration in seaweeds have been studied (Reddy et al 2008), the effect of light spectral quality has never been analyzed.…”

Cartilha ilustrativa sobre cultivo de macroalgas marinhas sob condições de laboratório

“…Objetivo do cultivo: isolar protoplastos e identificar efeitos dos principais fatores no rendimento de protoplastos para melhorar o protocolo de isolamento (Lafontaine et al 2011). Fonte da imagem: (https://www.algaebase.org/).…”

Effects of salinity and irradiance on early developmental stages of <italic>Grateloupia turuturu</italic> (Halymeniaceae, Rhodophyta) tetrasporophytes

“…It has a worldwide distribution and is considered an invasive species in many parts of the world (Villalard-Bohnsack and Harlin 1997, Saunders and Withall 2006, D'Archino et al 2007, de Azevedo et al 2015, Bolton et al 2016, Kraemer et al 2017, Petrocelli et al 2020. This alga has slippery blades that range from a dark red to pink to red-tinted brown, with extremely variable morphologies occurring in intertidal and subtidal environments (Simon et al 1999, Lafontaine et al 2011. They have a dis-coid holdfast (<5 mm wide), with a short (<1.5 mm long) cylindrical stipe, which widens out into a flattened blade (Villalard-Bohnsack and Harlin 1997).…”