Production and release of yessotoxins by the dinoflagellates Protoceratium reticulatum and Lingulodinium polyedrum in culture

Paz, Beatriz; Riobó, Pilar; Fernández, María Dolores; Fraga, Santiago; Franco, José M.

doi:10.1016/j.toxicon.2004.05.021

Cited by 186 publications

(127 citation statements)

References 15 publications

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“…For YTX of Protoceratium reticulatum, a significant amount was found in the culture medium, although the percentage of intracellular YTX of all strains at all experimental conditions was higher than extracellular YTX. This was observed as well for other strains of P. reticulatum (Paz et al, 2004(Paz et al, , 2013Guerrini et al, 2007;Rö der et al, 2012), although it has to be kept in mind that these authors did not report on extraction efficiency of their SPE method to quantify extracellular YTX and thus the true extracellular concentrations in their experiment might have been higher. A general feature observed for all Greenland isolates and in the studies cited above was an increase in intracellular YTX cell quota at stationary phase.…”

Section: Yessotoxinsmentioning

confidence: 63%

“…This might indicate an increased excretion of these compounds by actively growing cells, but it also might reflect an increased degradation of extracellular metabolites in old cultures as bacterial densities usually peak when algae become dense. In contrast, other studies (Paz et al, 2004;Guerrini et al, 2007) have found increasing per-cell extracellular concentrations of YTX in stationary phase, which is commonly discussed as a stress related release due to cell leakage or death. Clearly, more detailed studies are needed to fully evaluate the significance, variability, and potential role of extracellular YTX.…”

Section: Yessotoxinsmentioning

confidence: 74%

See 1 more Smart Citation

Growth and bioactive secondary metabolites of arctic Protoceratium reticulatum (Dinophyceae)

et al. 2016

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Section: Yessotoxinsmentioning

confidence: 63%

Section: Yessotoxinsmentioning

confidence: 74%

Growth and bioactive secondary metabolites of arctic Protoceratium reticulatum (Dinophyceae)

et al. 2016

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“…Other non-toxic strains of L. polyedrum from Norway, Spain and California (USA) have also been reported (Armstrong & Kudela, 2006). In contrast, toxic strains have been reported from Italy, the United Kingdom, Ireland, Galicia and Andalucía (Spain) (Paz, Riobó, Fernández, Fraga, & Franco, 2004;Paz et al, 2008) and California (USA) (Armstrong & Kudela, 2006).…”

Section: Discussionmentioning

confidence: 99%

The inhibitory effect of a non-yessotoxin-producing dinoflagellate, Lingulodinium polyedrum (Stein) Dodge, towards Vibrio vulnificus and Staphylococcus aureus

Quijano-Scheggia

Barajas-Gonzalez

Lim

et al. 2016

RBT

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Abstract:The increased bacterial resistance to antibiotics has caused global concern, prompting the search for new compounds. Because of their abundance and diversity, marine phytoplankton are an important potential source of such compounds. Research on dinoflagellates has led to the discovery of inhibitors of bacterial growth. The marine dinoflagellate Lingulodinium polyedrum blooms in different regions of the world, including Mexico, and is also known to regulate the growth of other species in coastal waters. Here, we investigated the taxonomy of this dinoflagellate and characterized the ability of its extracts to inhibit the growth of two bacteria of medical importance (Vibrio vulnificus and Staphylococcus aureus). Taxonomic characterization was performed by PCR and gene amplification of ITS, and confirmed that the species isolated off the Pacific coast of Mexico was L. polyedrum. To prove the inhibitory effect of L. polyedrum extracts, cultures were harvested by centrifugation. Pellets from three cellular abundances were extracted with water, methanol, hexane and chloroform. The experiments on V. vulnificus showed a high growth inhibition for the four extracts, ranging from 77 to 98 %. Surprisingly, the growth inhibition was lower when the extracts originated from a higher L. polyedrum cell abundance, ranging from 0 to 34 %. For S. aureus, the growth inhibition was also high, but not statistically different for all extracts and cell abundances, ranging from 62 to 99 %. This study obtained promising results for future pharmacological applications. Our Mexican strain of L. polyedrum did not produce any detectable yessotoxins. Rev. Biol. Trop. 64 (2): 805-816. Epub 2016 June 01.

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“…Contaminants affect the physiology of vegetative cells by acting on the levels of oxidative stress in cells (Okamoto and Colepicolo, 1998;Pinto et al, 2003) or by reducing their light-harvesting capacity and by inhibiting cell growth (Miao and Wang, 2006;Herzi et al, 2013). Under high levels of iron, cell Naple, Adriatic sea (Italy), Alfacs Bay (Spain) (Montresor, 1995;Bravo et al, 2006;Penna et al, 2010;Satta et al, 2013) Alexandrium minutum Halim * Paralytic shellfish toxins (Bravo et al, 2006) Harbour of Alexandria (Egypt), Catalonia, Arenys del mar (Spain); Ionian sea, Adriatic sea, Tyrrhenian sea, Olbia (Italy), Aegean Sea (Turkey) (Ismael and khadr, 2003;Anglès et al, 2010;Rubino et al, 2010;Penna et al, 2010;Aydin et al, 2011) Alexandrium catenella/tamarense (Whedon & Kofoid) Balech ** Paralytic shellfish toxins (Laabir et al, 2013) Aegean Sea (Turkey), Ionian Sea, Olbia, (Italy), Thau lagoon (France), Tarragona and Barcelona Harbours (Spain) (Bravo et al, 2006;Satta et al, 2010;Rubino et al, 2010;Aydin et al, 2011) Lingulodinium polyedrum (Stein) Dodge Lingulodinium sp ** Yessotoxin (Paz et al, 2004) Ionian Sea, Adriatic sea (Italy), Catalan sea, Arenys del mar, Alfacs and Fangar bays (Spain) Penna et al, 2010;Satta et al, 2010Satta et al, , 2013) Gonyaulax cf. spinifera complex (Claparéde et Lachmann) Diesing * Yessotoxin (Rhodes et al, 2006) Arenys del mar (Spain), Olbia (Italy) Protoceratium reticulatum (Claparède et Lachmann) Butschli * Yessotoxin (Paz et al, 2004) Adriatic sea (Italy), Alfacs and Fangar bays Satta et al, 2013) Protoperidinium claudicans (Paulsen) Balec ** Adriatic sea, Tyrrhenian sea, Olbia and Syracuse bay (Italy) Arenys harbour, Catalonia, Alfacs and Fangar bays (Spain) Garcés et al, 2010;Satta et al, 2013) Protoperidinium leonis * Non toxic Ionian sea ) Protoperidinium compressum (Abé) ...…”

Section: Correlation Between the Contaminants And Dinocyst Abundance mentioning

confidence: 99%

Do the levels of industrial pollutants influence the distribution and abundance of dinoflagellate cysts in the recently-deposited sediment of a Mediterranean coastal ecosystem?

Triki

Laabir

Lafabrie

et al. 2017

Science of The Total Environment

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We studied the relationships between sediment industrial pollutants concentrations, sediment characteristics and the dinoflagellate cyst abundance within a coastal lagoon by investigating a total of 55 sampling stations within the Bizerte lagoon, a highly anthropized Mediterranean ecosystem. The sediment of Bizerte lagoon is characterized by a high dinocyst abundance, reaching a maximum value of 2742 cysts·g −1 of dry sediment. The investigated cyst diversity was characterized by the presence of 22 dominant dinocyst morphotypes belonging to 11 genera. Two dinoflagellate species dominated the assemblage: Alexandrium pseudogonyaulax and Protoperidinium claudicans, representing 29 to 89% and 5 to 38% of the total cyst abundance, respectively, depending on the station. Seven morphotypes belonging to potentially toxic species were detected, including Alexandrium minutum, A. pseudogonyaulax, Alexandrium catenella/tamarense species complex, Lingulodinium polyedrum, Gonyaulax cf. spinifera complex, Prorocentrum micans and Protoceratium reticulatum. Pearson correlation values showed a positive correlation (α = 0.05) between cyst abundance and both water content and fine silt sediment content. Clustering revealed that the highest abundance of cysts corresponds to stations presenting the higher amounts of heavy metals. The simultaneous autoregressive model (SAM) highlighted a significant Contents lists available at ScienceDirectScience of the Total Environment j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m / l o c a t e / s c i t o t e n v correlation (α = 0.05) between cyst accumulation and two main factors: sediment water content and sediment content for several heavy metals, including Hg, Cd, Cu, Ni and Cr. These results suggest that the degree of heavy metal pollution could influence cyst accumulation patterns.

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Production and release of yessotoxins by the dinoflagellates Protoceratium reticulatum and Lingulodinium polyedrum in culture

Cited by 186 publications

References 15 publications

Growth and bioactive secondary metabolites of arctic Protoceratium reticulatum (Dinophyceae)

Growth and bioactive secondary metabolites of arctic Protoceratium reticulatum (Dinophyceae)

The inhibitory effect of a non-yessotoxin-producing dinoflagellate, Lingulodinium polyedrum (Stein) Dodge, towards Vibrio vulnificus and Staphylococcus aureus

Do the levels of industrial pollutants influence the distribution and abundance of dinoflagellate cysts in the recently-deposited sediment of a Mediterranean coastal ecosystem?

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