Production and shedding of channel catfish virus (CCV) and thymidine kinase negative CCV in immersion exposed channel catfish fingerlings

Kancharla, S. R.; Hanson, Larry A.

doi:10.3354/dao027025

Cited by 28 publications

(26 citation statements)

References 16 publications

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“…Likewise, fish exposed to CCV had higher neutralizing antibody activity than those exposed to CCVlacZ. Previous studies found that the ability of the TK-deleted recombinants to infect and replicate early during the infection was similar to the parent CCV, but then virus numbers dwindled more quickly (Zhang & Hanson 1995, Kancharla & Hanson 1996. This more rapid clearance may account for a lesser stimulation of specific antibody responses that we observed in the western blots.…”

supporting

confidence: 47%

“…RT-PCR products were analyzed by 10% acrylamide gel electrophoresis and GelStar staining. RT = reverse transcriptase; NO RT = without RT; +: positive control using purified CCV DNA; -: negative control using water was similar to the parent CCV, but then virus numbers dwindled more quickly (Zhang & Hanson 1995, Kancharla & Hanson 1996. This more rapid clearance may account for a lesser stimulation of specific antibody responses that we observed in the western blots.…”

Section: Discussionmentioning

confidence: 85%

“…In a previous study, channel catfish were infected with similar doses of CCV or a TK-negative mutant and demonstrated that 100% of 16 sampled fish developed disseminated viremia by 4 d post infection and this progressed to latent infections in the survivors (as defined by presence of detectable CCV genome but no infectious virus) by 12 d post exposure (Kancharla & Hanson 1996). However, the authors did not attempt to induce recrudescence or evaluate the immune response over time.…”

Section: Discussionmentioning

confidence: 99%

“…Also primary exposure to CCV appears to more frequently result in a latent carrier state than a lethal form of disease. In field studies, population prevalence of CCV latency increased over time with no overt disease (Thompson et al 2005) and in experimental challenges, exposure to low levels of virus resulted in disseminated virus replication but low mortality (Kancharla & Hanson 1996). Since reactivation is a complex phenomenon that is infrequent unless an appropriate stimulus is applied, evaluation of the expression of RNA necessary for activation of the lytic cascade and production of infectious particles should be more sensitive during the early events of reactivation than culture.…”

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

“…The remaining 400 µl of PBL suspension were cultured in separate wells of a 6-well tissue culture plate containing 70% confluent CCO cell monolayers. Tissue cultures were maintained at 30°C in DMEM (without sodium bicarbonate) supplemented with 10% fetal calf serum and 25 mM HEPES, and observed daily for 10 d for cytopathic effect (CPE) as previously described by Kancharla & Hanson (1996). They were then blind-passaged by transferring supernatant fluids to a new plate of CCO cells at 70% confluency.…”

Section: Serum Neutralization Index Test (Sni)mentioning

confidence: 99%

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Antibody response of channel catfish after channel catfish virus infection and following dexamethasone treatment

Arnizaut¹,

Hanson²

2011

Dis. Aquat. Org.

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Channel catfish virus (CCV, Ictalurid herpesvirus 1) and CCV disease have been extensively studied. Yet, little is known about CCV-host interaction after resolution of the primary infection. In order to determine potential recrudescence of CCV from latency, we established latency by exposing channel catfish juveniles with CCV or a thymidine kinase-negative recombinant (CCVlacZ) at a dose that caused less than 20% mortality. Then, we evaluated antibody response by serially sampling the same fish at 0 (pre-infection), 30, 60 and 90 d post challenge (DPC). We then attempted to induce viral recrudescence by intramuscular administration of dexamethasone and sampled the fish at 2, 4, 7, or 10 d post treatment. Recrudescence was evaluated by leukocyte co-cultivation and cell culture of tissue homogenates but no virus was detected. Western blot data demonstrated the highest number of seropositive fish by 30 DPC and a secondary antibody induction after dexamethasone treatment. The antigen specificity of the secondary response corresponded to viral proteins with molecular masses similar to those recognized by the same fish by 30 DPC. The recognized proteins were predominantly large, ranging from ~90 to > 200 kDa. Expression analysis of selected virus genes at 90 DPC and following dexamethasone treatment demonstrated occasional immediate-early virus gene expression in peripheral blood leukocytes. Early and late gene expression was rarely detected. The combined data suggest restricted re-activation of CCV in our experimental system. Primary and secondary responses and virus gene expression were demonstrated in CCVlacZ-exposed fish but were less frequent than in CCV-exposed fish.KEY WORDS: Ictalurus punctatus · Teleost · Ictalurid herpesvirus 1 · Alloherpesviridae · Herpesvirus latency · Thymidine kinase-negative recombinant · Viral gene expression · Neutralizing antibody Resale or republication not permitted without written consent of the publisherDis Aquat Org 95: [189][190][191][192][193][194][195][196][197][198][199][200][201] 2011 plex type 1 (HSV-1, Human herpesvirus 1), is characterized by expression of immediate-early (IE), early (E), and late (L) classes of viral genes in an ordered lytic cascade that culminates in the replication and packaging of viral DNA into infectious particles, and eventual lysis of the infected cells (Honess & Roizman 1974). With respect to the kinetics of gene expression, Dixon & Farber (1980) observed similar cascade regulation of the synthesis of 3 distinct classes of CCV proteins.Basic components of the morphology, biology, and genomic structure of CCV (Wolf & Darlington 1971, Plumb & Gaines 1975, Chousterman et al. 1979, Davison 1992 have been characterized since this fish herpesvirus was first isolated by Fijan (1968). CCV encodes thymidine kinase (TK), which phosphorylates thymidine and other deoxynucleotides and analogs (Hanson & Thune 1993). For HSV-1, TK is not required for growth in cell culture but is essential for virulence (Jamieson et al. 1974, Field & Wildy 1978, ...

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supporting

confidence: 47%

Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

Section: Serum Neutralization Index Test (Sni)mentioning

confidence: 99%

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