Production and stability of water‐dispersible astaxanthin oleoresin from Phaffia rhodozyma

Abstract

The process of extracting the astaxanthin oleoresin from pretreated Phaffia rhodozyma cells was optimised using a Box-Behnken response surface design. Microwaving the cells at 105 W for 1 min followed by ethyl acetate extraction was the best pretreatment, and the optimal extraction conditions were 65°C for 24 min using a solvent-solid ratio of 19:1. The order of the ability to disperse the astaxanthin oleoresin was propylene glycol> Tween 80 > Tween 20 > a-cyclodextrin, b-cyclodextrin. It was determined that the degradation of the colour of the water-dispersible oleoresin followed a first-order kinetics model. The greatest stability was observed at pH 4 and at the lowest temperature evaluated (40°C). The thermal degradation of the pigment occurs in two steps, the first one from 0 to 1.5 h, with an E a I = 10.31 kJ mol À1 , and the second one from 1.5 to 5 h, with an E a II = 30.06 kJ mol À1

Astaxanthin as feed supplement in aquatic animals

“…Conversely, the bioavailability of astaxanthin was higher when used with protein-based ingredients, especially milk. The greatest stability of astaxanthin oleoresin from P. rhodozyma dispersed in aqueous solution of propylene glycol was noted at pH 4 and at low temperatures (Villalobos-Castillejos et al 2013). Moreover, Peng et al (2010) observed improved stability and bioavailability of astaxanthin when encapsulated within liposomes.…”

“…The greatest stability of astaxanthin oleoresin from P. rhodozyma dispersed in aqueous solution of propylene glycol was noted at pH 4 and at low temperatures (Villalobos‐Castillejos et al . ). Moreover, Peng et al .…”

Rapidly colorimetric detection of caffeine in beverages by silver nanoparticle sensors coupled with magnetic molecularly imprinted polymeric microspheres

“…Extraction by organic solvents, centrifugation, macroporous resin separation and solid‐phase extraction with cartridges are frequently used for sample pretreatment (Lichtenberg et al ., ; Villalobos‐Castillejos et al ., ). For example, a gas chromatography with nitrogen–phosphorus detector analytical method was reported to determine caffeine isolated from the coffee by liquid–liquid extraction before analysing (Mccusker et al ., ).…”

Highly efficient preparation of free all‐trans‐astaxanthin from Haematococcus pluvialis extract by a rapid biocatalytic method based on crude extracellular enzyme extract

“…Chemical synthesis of astaxanthin would inevitably lead to the introduction of toxic chemical impurities. Therefore, it is preferentially obtained from natural sources such as the Haematococcus pluvialis ( H. pluvialis ) (Kim et al ., ), the yeast Phaffia rhodozyma (Villalobos‐Castillejos et al ., ), or shrimp, crab and other crustaceans (Mao et al ., ). Astaxanthin has a variety of geometric isomers, such as 9‐ cis , 13‐ cis and all‐ trans , of which all‐ trans configuration is the most stable structure (Fig.…”