Production and stability studies of the biosurfactant isolated from marine Nocardiopsis sp. B4

Khopade, Abhijeet; Ren, Biao; Liu, X.; Mahadik, Kakasaheb R.; Zhang, Lixin; Kokare, Chandrakant

doi:10.1016/j.desal.2011.10.002

Cited by 197 publications

(128 citation statements)

References 30 publications

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“…Thermal stability studies of biosurfactant from B. subtilis UKMP-4M5 indicated that the surfactant was thermostable, as heating up to 100 °C caused no significant effect on the biosurfactant performances. The high thermal stability of the test biosurfactant is on par with other synthetic surfactants such as linear alkylbenzene sulfonate (LAS) and alkylpolyglucoside [45], but superior to sodium dodecyl sulphate, which exhibits a significant loss of activity at temperatures above 70 °C [46]. Interestingly, the test biosurfactant retained its surface activity even after autoclaving at 121 °C for 20 min, suggesting its potential application in food, pharmaceutical and cosmetic industries where heating to achieve sterility are important.…”

Section: Identification Of Biosurfactant-producing Bacteriamentioning

confidence: 77%

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DETERMINATION OF OPTIMUM CONDITIONS AND STABILITY STUDY OF BIOSURFACTANT PRODUCED BY Bacillus subtilis UKMP-4M5

Hamzah¹

2016

MJAS

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Bacillus subtilis UKMP-4M5 was isolated from a hydrocarbon contaminated site which was found to be the most potential biosurfactant producer. Isolates were grown in a mineral salt medium (MSM) supplemented with sunflower oil (1% v/v) as the main carbon source. Screening was based on both qualitative (oil spreading technique) and quantitative (emulsification index and surface tension measurement) methods. B. subtilis UKMP-4M5 produced the highest oil displacement activity with and emulsification index of 17%. It also reduced surface tension of culture medium from 58.95 to 41.75 mN/m. B. subtilis UKMP-4M5 produced biosurfactant with surface tension measurement of 32.7 ± 0.66 mN/m using 2% (v/v) palm oil and 0.5% (w/v) yeast extract as carbon and nitrogen sources respectively. The tested biosurfactant exhibited excellent thermal (up to 120 °C) and pH (6.0 -8.0) stability as well as high tolerance for varying salt concentrations (1 -5% w/v) in terms of surface tension reducing ability. This suggests potential applications in fields such as enhanced oil recovery, bioremediation, and the food industry. Keywords: Bacillus subtilis, biosurfactant, palm oil, surface tension AbstrakBacillus subtilis UKMP-4M5 yang dipencilkan dari kawasan yang tercemar dengan hidrokarbon didapati paling berpotensi sebagai penghasil biosurfaktan. Pencilan dihidupkan di dalam medium garam mineral (MSM) yang ditambahkan dengan 1% (i/i) minyak bunga matahari sebagai sumber karbon utama. Penyaringan adalah berdasarkan kepada kedua-dua kaedah kualitatif (teknik penyebaran minyak) dan kuantitatif (indeks emulsifikasi dan ukuran ketegangan permukaan). B. subtilis UKMP-4M5 merekodkan aktiviti sesaran minyak yang tertinggi dengan indeks emulsifikasi sebanyak 17%. Ia juga merendahkan ketegangan permukaan kultur medium dari 58.95 mN/m ke 41.75 mN/m B. subtilis UKMP-4M5 menghasilkan biosurfaktan dengan ukuran ketegangan permukaan 32.7 ± 0.66 mN/m menggunakan 2% (i/i) minyak kelapa sawit dan 0.5% (b/i) ekstrak yis masing-masing sebagai sumber karbon dan nitrogen. Biosurfaktan yang diuji menunjukkan kestabilan yang amat baik pada suhu (sehingga 120 °C) dan pH (6.0 -8.0) serta toleransi yang tinggi pada kepekatan garam yang berbeza (1 -5% b/i) dari segi keupayaannya merendahkan ketegangan permukaan. Ini mencadangkan potensi aplikasinya dalam industri seperti peningkatan perolehan minyak, bioremediasi dan industri makanan.

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Section: Identification Of Biosurfactant-producing Bacteriamentioning

confidence: 77%

“…Similar results had been reported for biosurfactant production from other microorganisms such as P. aeruginosa [14], which was stable at a wide range of pH between 6.0-10.0, whereas stability of biosurfactant from Nocardiopsis sp. was found to be stable between pH 8 -12 [46].…”

Section: Identification Of Biosurfactant-producing Bacteriamentioning

confidence: 92%

DETERMINATION OF OPTIMUM CONDITIONS AND STABILITY STUDY OF BIOSURFACTANT PRODUCED BY Bacillus subtilis UKMP-4M5

Hamzah¹

2016

MJAS

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“…Highly insoluble carbon source such as n-hexadecane, paraffinic oil, glycerol, babassu oil for P. aeruginosa PA1 [28], soybean curd residue (okara) for B. subtilis YB8 and B. subtilis MI113 [29,30], peat hydrolysate for B. subtilis [31], soybean oil, safflower oil, glycerol for P. aeruginosa GS9-119 and DS10-129 [32] and sludge palm oil for Klebsiella pneumoniae WMF02 [33] have been reported to induce high biosurfactant production. Olive oil was an unconventional carbon source used for the production of lipopeptide biosurfactant by Bacillus subtilis SK320 [3] and biosurfactant produced by marine Nocardiopsis B4 [34]. The important finding was the temperature stability of both the biosurfactants increasing their scope of application at higher temperatures like in microbial enhanced oil recovery.…”

Section: Improvement In Biosurfactant Production and Their Applicationsmentioning

confidence: 99%

Biosurfactant Production and Potential Correlation with Esterase Activity

Sekhon¹

2012

J Pet Environ Biotechnol

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Biosurfactants (microbial surfactants) are surface active compounds produced extracellularly or as part of the cell membrane by several bacterial and fungal species. They have the unique property of reducing the surface and interfacial tension of liquids. Biosurfactants have applications in the field of agriculture, petroleum, microbial enhanced oil recovery, biomedical sciences, cosmetics, food processing and pharmaceuticals. The global biosurfactants market has grown gradually. Regardless of their greater biodegradability and reduced toxicity, cost competitiveness still remains the major concern for biosurfactant production. However, recombinant or metabolically engineered hyper producing strains combined with optimized cultivation conditions have made it possible for many companies to reap the benefits of 'green' biosurfactant technology. Simultaneously, biosurfactants and bioemulsifiers showing esterase activities and having potential applications are reported to form stable oil-water emulsions with hydrophobic substrates such as hexadecane and polyaromatic hydrocarbons. Biosurfactant production and release of esterases by the microbial cells is shown to be synchronized and symbiotically beneficial in some species. Several bacterial biosurfactant and esterase genes have been identified, cloned and expressed for their enhanced production. This

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“…Also the industrial application of biosurfacants is limited by a lack of public acceptance of the biosurfactant-producing microorganisms (Zhang et al, 2012;Xu et al, 2011). …”

Section: Introductionmentioning

confidence: 99%

Production, Characterization and Application of a New Biosurfactant Derived from Egyptian Sunflower Seeds

2016

IJSR

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Abstract:A new biosurfactant (SR122) was obtained for the first time from the seeds of sunflower plant growing locally in Egypt. This bioproduct was recovered from the seeds using simple and cost effective method that gave higher biosurfactant yield (20-25% w/w). The new product (SR 122) may be an alternative to the microbial and synthetic surfactants because it is non toxic (as it was originated from safe natural sources-the plant), cost-effective, produced in higher yield (as compared to the microbial biosurfactant) and may acquire public acceptance. The high surface activity of SR 122 (as measured by the ODA method, surface tension reduction and E24 method), the non-toxicity and tolerance to wide range of harsh condition of temperatures (up to 121ºc), pH values (2-11) and different NaCl concentrations (2% -25% w/v) make this new product a promising candidate for the application in many industries and for the protection of the environment. On a large laboratory scale SR122 was applied for cleaning oil storage tank. During the cleaning process more than 95% of the oily sludge was recovered and the viscosity of the recovered oil was decreased allowing the oily sludge to be easily floated on the top of the biosurfactant as a distinct phase. This product SR 122 was offered for a patent no: 1289/2014

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Production and stability studies of the biosurfactant isolated from marine Nocardiopsis sp. B4

Cited by 197 publications

References 30 publications

DETERMINATION OF OPTIMUM CONDITIONS AND STABILITY STUDY OF BIOSURFACTANT PRODUCED BY Bacillus subtilis UKMP-4M5

DETERMINATION OF OPTIMUM CONDITIONS AND STABILITY STUDY OF BIOSURFACTANT PRODUCED BY Bacillus subtilis UKMP-4M5

Biosurfactant Production and Potential Correlation with Esterase Activity

Production, Characterization and Application of a New Biosurfactant Derived from Egyptian Sunflower Seeds

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