Production and Structural Characterization ofLactobacillus helveticusDerived Biosurfactant

Abstract

A probiotic strain of lactobacilli was isolated from traditional soft Churpi cheese of Yak milk and found positive for biosurfactant production. Lactobacilli reduced the surface tension of phosphate buffer saline (PBS) from 72.0 to 39.5 mNm−1 pH 7.2 and its critical micelle concentration (CMC) was found to be 2.5 mg mL−1. Low cost production of Lactobacilli derived biosurfactant was carried out at lab scale fermenter which yields 0.8 mg mL−1 biosurfactant. The biosurfactant was found least phytotoxic and cytotoxic as compared to the rhamnolipid and sodium dodecyl sulphate (SDS) at different concentration. Structural attributes of biosurfactant were determined by FTIR, NMR (1H and 13C), UPLC-MS, and fatty acid analysis by GCMS which confirmed the presence of glycolipid type of biosurfactant closely similar to xylolipids. Biosurfactant is mainly constituted by lipid and sugar fractions. The present study outcomes provide valuable information on structural characterization of the biosurfactant produced by L. helveticus MRTL91. These findings are encouraging for the application of Lactobacilli derived biosurfactant as nontoxic surface active agents in the emerging field of biomedical applications.

Biosurfactants from probiotic bacteria: A review

“…For all of the studied strains, the levels of cell‐bound biosurfactant production were found to be higher than the excreted ones, as it was previously described for other lactobacilli . On the contrary, other microorganisms (such as Bacillus and Pseudomonas species) produce mainly excreted biosurfactants, which are secreted into the medium, leading to substantial decreases in its ST .…”

Characterization and Antibacterial Potential of Lactic Acid Bacterium Pediococcus pentosaceus 4I1 Isolated from Freshwater Fish Zacco koreanus

“…However, the synergistic effects of acids, hydrogen peroxide, and bacteriocin-like substances cannot be ruled out (Anas et al, 2008). Sjögren et al (2003) also confirmed the antimicrobial properties of 3-(R)-hydroxydecanoic acid, 3-hydroxy-5- cis -dodecenoic acid, 3-(R)-hydroxydodecanoic acid, and 3-(R)-hydroxytetradecanoic acid, from Lactobacillus plantarum MiLAB 14, and Sharma et al (2014) confirmed the presence of octadecanoic acid in Lactobacillus helveticus by GC–MS. Haque et al (2009) reported that of the organic acids examined, propionic acid (PA) was an effective antimicrobial, and thus, further supported the organic acid-mediated inhibitory effect of Lactobacilli strains, and the outcome of the present study.…”

Biosurfactant/s from Lactobacilli species: Properties, challenges and potential biomedical applications

“…do possess cytotoxic effect. This is supported by Sharma et al who investigated the cytotoxic effect of L. helveticus ‐derived biosurfactant using a mouse fibroblast (ATCC L929) cell line. They reported that as the concentration of biosurfactant increased (6.25–25 mg ml −1 ) there was a gradual decreased in cell viability.…”

“…The presence of methyl esters glycolipid biosurfactant was correlated to an increased hydrophobicity and, as a result enhancing not only the biosurfactant surface activity but also hemolytic and antifungal activities. Sharma et al reported again glycolipid‐type biosurfactant from Lactobacilli spp. having mixture of sugar and lipid fractions which was claimed to be similar to xylolipid.…”