Production by Bacillus pumilus (MSH) of an antifungal compound that is active against Mucoraceae and Aspergillus species: preliminary report

Bottone, Edward J.; Peluso, Richard W.

doi:10.1099/jmm.0.04935-0

Cited by 68 publications

(50 citation statements)

References 12 publications

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“…This is in agreement with bibliographical data showing that Bacillus produces antimicrobial substances of protein nature (Wu et al, 2005). These molecules are essentially active against Gram-positive bacteria, nevertheless compounds such as polymixin and colistin inhibit Gram-negative forms (Bottone and Peluso, 2003), whereas Bacillomycin, Mycobacillin, and Fungistatin are active against yeasts and fungi (Katz and Demain, 1977). Bottone and Peluso (2003) have reported the inhibition of species from the genera Mucoraceae and Aspergillus by an antifungal compound produced by B. pumilus.…”

Section: Microorganismsupporting

confidence: 76%

In vitro and intracellular antimycobacterial activity of a Bacillus pumilus strain

Hassi¹,

David²,

Haggoud³

et al. 2012

Afr. J. Microbiol. Res.

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Despite the declaration of tuberculosis (TB) as a global emergency by the world health organization (WHO) about 20 years ago, the worldwide problem of this disease has worsened due to increased drug resistance of tuberculosis bacilli and acquired immune deficiency syndrome (AIDS) pandemic. Consequently, fight against multidrug and extensively drug-resistant TB is a high priority for public health and research. The present work describes the isolation of a Bacillus pumilus strain secreting a metabolite of protein nature capable of inhibiting mycobacterial growth (Mycobacterium smegmatis, Mycobacterium aurum and Mycobacterium bovis BCG). This metabolite is not toxic, accumulates within the macrophage and inactivates the bacilli with a comparable efficiency to that of the pure commercial antimycobacterial substance Amikacin.

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Section: Microorganismsupporting

confidence: 76%

In vitro and intracellular antimycobacterial activity of a Bacillus pumilus strain

Hassi¹,

David²,

Haggoud³

et al. 2012

Afr. J. Microbiol. Res.

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“…[21] In the search for antibiotics produced by Bacillus species, especially Bacillus cereus, Bacillus subtilis and Bacillus licheniformis, several antifungal compounds, mainly peptides, have also been described. [22,23,24,25] Bottone & Peluso [26] have reported a compound produced by Bacillus pumilus (MSH) that inhibits…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity of Bacillus Sp. Jg: Isolation, Identification and Process Optimization for the Production of Antimicrobial Metabolite Against Human Pathogens

Jain¹

2017

WJPPS

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Antibiotic resistance in bacteria has become a serious problem. Therefore, the search for new antibiotics is an important endeavour and is very much needed. In the present investigation, a number of bacterial strains were isolated from different soil samples and were tested for antimicrobial metabolite production. 85 bacterial isolates were isolated and the best activity was exhibited by the bacterial isolate P13. Physiological, biochemical and 16S rDNA gene sequence analysis strongly suggested that the isolate P13 belonged to Bacillus sp. Effect of medium, incubation period, pH and temperature variations for the maximum production of antimicrobial metabolite were optimized for maximum antimicrobial metabolite production. The maximum production of antimicrobial metabolite was observed in WYH under pH 7.0 and incubation temperature of 35 o C. Nutritional and culture conditions for the production of antimicrobial metabolite by this organism under shake-flask conditions were also optimized. Dextrose and peptone were found to be the best carbon and nitrogen sources for the production of antimicrobial metabolite by Bacillus sp. JG. Chloroform was the best extraction solvent for the isolation of the antimicrobial metabolite. The minimum inhibitory concentration of the metabolite against the test pathogens ranged between 0.9 to 2mg/ml. It may be suggested from the present study that further research is needed for World Journal of Pharmacy and Pharmaceutical Sciencesdetermining the chemical structure of metabolite which is responsible for bio-efficacy and to determine the cytotoxicity before it is used for commercialization purposes.

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“…The exact size of the plasmid could only be ascertained on a pulse field gel electrophoresis since the resolution of agarose gel electrophoresis is ∼23 kb but the modified in situ plasmid isolation method too revealed the size of the plasmid to be equivalent to plasmid of E.coli S17, which is 23 kb. Biological & physiochemical characterization of Bacillus pumillus and its antifungal compounds have been described earlier [19] but presence of plasmid in Bacillus pumillus has not been reported so far. Bacillus pumillus MTCC7615 is resistant to antibiotic naldixic acid and it was observed that antibiotic naldixic acid resistance gene is associated with the plasmid pJCP07 of Bacillus pumillus MTCC7615 since curing the isolate of its plasmid pJCP07 made it naldixic acid-sensitive.…”

Section: Pcr Studies To Confirm Presence Of Tn5mentioning

confidence: 99%

Molecular characterization of soil bacteria antagonistic toRhizoctonia solani, sheath blight of rice

Padaria

Singh

2009

Journal of Environmental Science and Health, Part B

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Bacillus pumillus MTCC7615 has been identified as a potent isolate against Rhizocotonia solani, the fungal pathogen causing sheath blight in rice. The study aimed at probing the role of a 23kb size plasmid pJCP07 of Bacillus pumillus MTCC7615 in its fungal antagonism towards Rhizocotonia solani. Plasmid pJCP07 was found to be involved in production of a fungal antagonistic compound as demonstrated by plasmid curing and conjugational transfer experiments. Tn5 insertional studies further confirmed that the plasmid pJCP07 of Bacillus pumillus MTCC7615 carries some of the gene(s) involved in production of compound antagonistic to Rhizocotonia solani. The plasmid pJCP07 is thus a mobilizable medium-sized plasmid carrying genes responsible for antagonism of Bacillus pumillus MTCC7615 towards Rhizocotonia solani.Keywords: Bioantagonism; Bacillus pumillus MTCC7615; Rhizocotonia solani; plasmid curing; conjugational mating; Tn5 mutagenesis. IntroductionRice is one of the most important staple food crops for more than half of the world's population. In India rice is a major contributor to national food and nutritional security occupying around 23.3% of its gross cropped area and about 40% of its total food grain production.[1] Unfortunately, the net production of rice is greatly reduced due to losses caused by a number of diseases and sheath blight caused by fungal pathogenRhizoctonia solani is a devastating disease accounting for 24% to 50% economic losses across the rice cultivation zones of the world.[2] Control strategies available for this disease are limited, relying mainly on fungicide applications, which have significant deleterious environmental effects. Breeding resistance to sheath blight into rice crop is difficult because no known source of stable genetic resistance for this rice disease has been yet identified.[3] Thus alternative approaches involves utilization and genetic manipulation of microbes that synthesize compounds capable of control plant pathogen naturally in all agriculture ecosystems for organic and ecofriendly agriculture.[4] A number of microbes such as Pseudomonas, Serratia spp. etc. have already been reported to inhibit the growth of various fungal pathogens. [5] Microbes harbor plasmids as well as megaplasmids and these plasmids have several set of genes involved in mobilization, determination of fertility, controlling of cell structure and determination of metal tolerance, nodulation, environmental pollutant degradation ability etc. [6] A bacterial isolate, Bacillus pumillus MTCC7615 from a rice field was characterized as an antagonistic against Rhizoctonia solani under in vitro conditions. [7] Bacillus pumillus is known to produce antifungal metabolites which inhibit mycelial growth as well as spore germination of a number of pathogenic fungi as Aspergillus, Penincillium andFusarium species.[8] Characterizations of these metabolites have been carried out but reports on molecular studies of Bacillus pumillus strains are limited. Molecular characterization of Bacillus pumillus MTCC7615 was ...

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Production by Bacillus pumilus (MSH) of an antifungal compound that is active against Mucoraceae and Aspergillus species: preliminary report

Cited by 68 publications

References 12 publications

In vitro and intracellular antimycobacterial activity of a Bacillus pumilus strain

In vitro and intracellular antimycobacterial activity of a Bacillus pumilus strain

Antimicrobial Activity of Bacillus Sp. Jg: Isolation, Identification and Process Optimization for the Production of Antimicrobial Metabolite Against Human Pathogens

Molecular characterization of soil bacteria antagonistic toRhizoctonia solani, sheath blight of rice

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