Production, characteristics and applications of phytase from a rhizosphere isolated Enterobacter sp. ACSS

Abstract

Optimization of process parameters for phytase production by Enterobacter sp. ACSS led to a 4.6-fold improvement in submerged fermentation, which was enhanced further in fed-batch fermentation. The purified 62 kDa monomeric phytase was optimally active at pH 2.5 and 60 °C and retained activity over a wide range of temperature (40-80 °C) and pH (2.0-6.0) with a half-life of 11.3 min at 80 °C. The kinetic parameters K m, V max, K cat, and K cat/K m of the pure phytase were 0.21 mM, 131.58 nmol mg(-1) s(-1), 1.64 × 10(3) s(-1), and 7.81 × 10(6) M(-1) s(-1), respectively. The enzyme was fairly stable in the presence of pepsin under physiological conditions. It was stimulated by Ca(+2), Mg(+2) and Mn(+2), but inhibited by Zn(+2), Cu(+2), Fe(+2), Pb(+2), Ba(+2) and surfactants. The enzyme can be applied in dephytinizing animal feeds, and the baking industry.

Probiotic properties of a phytase producing Pediococcus acidilactici strain SMVDUDB2 isolated from traditional fermented cheese product, Kalarei

“…The enzyme reported has shown maximum substrate specificity with sodium phytate as in case of Lactobacillus coryniformis wherein the values of K m and V max were 0.32 mM and 4.83 μmol/min respectively 38 . A good thermostability of an enzyme, an important criteria of interest growing in industrial as well as scientific communities towards thermo-acido-philic enzymes 17 has been fulfilled in case of P. acidilactici SMVDUDB2.…”

“…The model was validated by performing the experiment under optimum value of 4 significant variables, peptone (1.27%); temperature (37 °C); pH (6.26) and maltose (1.43%) and the precise closeness of predicted response (5.563 U/mL) and actual response (5.583 U/ mL) for phytase production, indicated the suitability of the model. Statistical model has been used to enhance the production of phytase from several microorganisms [15][16][17] but, here in we are reporting the optimization of extracellular phytase from P. acidilactici using RSM.…”

“…For studying qualitative phytate degrading ability plate assay method was followed 17,40 . The phytase screening media (PSM) was composed of 1.5% glucose, 0.1% sodium phytate, 0.2% NH 4 NO 3 , 0.05% KCl, 0.05% MgSO 4 .7H 2 O, 0.03% MnSO 4 , 0.03% FeSO 4 .7H 2 O and 2.0% agar (pH 7.5).…”

“…After 5 min of incubation at room temperature the cobalt chloride solution was replaced with a freshly prepared solution containing equal volumes of 6.25% (w/v) aqueous ammonium molybdate) solution and 0.42% (w/v) ammonium meta vanadate solution. After 5 min incubation, the ammonium molybdate/ammonium vanadate solution was removed and the plates were examined for zone of hydrolysis 17 .…”

Effects of supplementation of high‐dosing Trichoderma reesei phytase in the corn‐wheat‐soybean meal‐based diets on growth performance, nutrient digestibility, carcass traits, faecal gas emission, and meat quality in growing‐finishing pigs

“…When used to feedstuffs, the stability was >85% after storage in 25°C environment for 3 months. The optima pH was 3.5–4.5 (Chanderman, 2016).…”