Production, characteristics and applications of microbial heparinases

Boyce, Angela; Walsh, Gary

doi:10.1016/j.biochi.2022.03.011

Cited by 14 publications

(6 citation statements)

References 251 publications

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“…LMW heparin, which has taken the role of heparin in various clinical uses, is produced by the industrial exploitation of heparinases' capacity to cleave heparin chains selectively. Heparinases are also used to analyze the structural properties of heparin and heparan sulfate, neutralize heparin in blood, and remove heparin's inhibitory effects on other enzymes [66].…”

Section: Endothelial Cell Functionmentioning

confidence: 99%

Correlation between Hepatocyte Growth Factor (HGF) with D-Dimer and Interleukin-6 as Prognostic Markers of Coagulation and Inflammation in Long COVID-19 Survivors

Zaira,

Yulianti,

Levita

2023

CIMB

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In general, an individual who experiences the symptoms of Severe Acute Respiratory Syndrome Coronavirus 2 or SARS-CoV-2 infection is declared as recovered after 2 weeks. However, approximately 10–20% of these survivors have been reported to encounter long-term health problems, defined as ‘long COVID-19’, e.g., blood coagulation which leads to stroke with an estimated incidence of 3%, and pulmonary embolism with 5% incidence. At the time of infection, the immune response produces pro-inflammatory cytokines that stimulate stromal cells to produce pro-hepatocyte growth factor (pro-HGF) and eventually is activated into hepatocyte growth factor (HGF), which helps the coagulation process in endothelial and epithelial cells. HGF is a marker that appears as an inflammatory response that leads to coagulation. Currently, there is no information on the effect of SARS-CoV-2 infection on serum HGF concentrations as a marker of the prognosis of coagulation in long COVID-19 survivors. This review discusses the pathophysiology between COVID-19 and HGF, IL-6, and D-dimer.

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Section: Endothelial Cell Functionmentioning

confidence: 99%

Correlation between Hepatocyte Growth Factor (HGF) with D-Dimer and Interleukin-6 as Prognostic Markers of Coagulation and Inflammation in Long COVID-19 Survivors

Zaira,

Yulianti,

Levita

2023

CIMB

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“…We then envisioned the possibility of the selective cleavage of trisaccharide 12 by heparinase III that has been known primarily for acting at the glycosidic bond between N -acetylated or N -sulfated glucosamine (GlcNAc or GlcNS) and a non-sulfated GlcA moiety, by an elimination mechanism, to depolymerize heparin and heparan sulfate (HS). 22 The cleavage reactivity of heparinase III on the PNZ-protected trimer 12 is shown in Fig. 2A.…”

Section: Resultsmentioning

confidence: 99%

Facile chemoenzymatic synthesis of unmodified anticoagulant ultra-low molecular weight heparin

et al. 2022

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“…Albeit more or less specific, HP and HS can be depolymerized from polysaccharides into disaccharides using different classes of bacterial heparinases I, II, and III. 11 Heparin oligosaccharides generated from heparinase I digestion will feature sulfated extremities. In contrast, the digestion of HS with heparinase III will display nonsulfated terminal saccharide units.…”

Section: Glycosaminoglycansmentioning

confidence: 99%

Glycosaminoglycans: What Remains To Be Deciphered?

Pérez

Makshakova

Angulo

et al. 2023

JACS Au

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Glycosaminoglycans (GAGs) are complex polysaccharides exhibiting a vast structural diversity and fulfilling various functions mediated by thousands of interactions in the extracellular matrix, at the cell surface, and within the cells where they have been detected in the nucleus. It is known that the chemical groups attached to GAGs and GAG conformations comprise “glycocodes” that are not yet fully deciphered. The molecular context also matters for GAG structures and functions, and the influence of the structure and functions of the proteoglycan core proteins on sulfated GAGs and vice versa warrants further investigation. The lack of dedicated bioinformatic tools for mining GAG data sets contributes to a partial characterization of the structural and functional landscape and interactions of GAGs. These pending issues will benefit from the development of new approaches reviewed here, namely (i) the synthesis of GAG oligosaccharides to build large and diverse GAG libraries, (ii) GAG analysis and sequencing by mass spectrometry (e.g., ion mobility-mass spectrometry), gas-phase infrared spectroscopy, recognition tunnelling nanopores, and molecular modeling to identify bioactive GAG sequences, biophysical methods to investigate binding interfaces, and to expand our knowledge and understanding of glycocodes governing GAG molecular recognition, and (iii) artificial intelligence for in-depth investigation of GAGomic data sets and their integration with proteomics.

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Production, characteristics and applications of microbial heparinases

Cited by 14 publications

References 251 publications

Correlation between Hepatocyte Growth Factor (HGF) with D-Dimer and Interleukin-6 as Prognostic Markers of Coagulation and Inflammation in Long COVID-19 Survivors

Correlation between Hepatocyte Growth Factor (HGF) with D-Dimer and Interleukin-6 as Prognostic Markers of Coagulation and Inflammation in Long COVID-19 Survivors

Facile chemoenzymatic synthesis of unmodified anticoagulant ultra-low molecular weight heparin

Glycosaminoglycans: What Remains To Be Deciphered?

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