Production, characterization and applications of proteases produced by Bacillus licheniformis, Acinetobacter pittii and Aspergillus niger using neem seed oil cake as the substrate

Reddy, N. Mal; Deekonda, Vidyavathi; Seshagiri, Swetha; Reddy, Roopa; Gangula, Ambika Kanakayya

doi:10.1016/j.indcrop.2022.115403

Cited by 16 publications

(4 citation statements)

References 38 publications

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“…Vannabun et al (2014) reported higher EA of alkaline protease enzyme extracted from giant catfish viscera compared to acid protease [20]. Similar studies on protease enzymes extracted from other surces, such as plants and microbes, have also shown that optimum EA was achieved at pH of 7-9 and temperature of 50°C [21][22][23][24]. These findings suggested that alkaline protease likely played a dominant role in the protease enzyme extracted with neutral solvent.…”

Section: Enzyme Activity Of Crude Enzymementioning

confidence: 60%

Ultraviolet irradiation effect on characteristics of protease enzyme from African catfish (Clarias sp.) intestines

Tamaya,

Suwanto,

Hernawan

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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The goal of this study was to investigate the impact of UV-C irradiation on the activity of crude enzymes derived from African Catfish intestines as well the optimum conditions for subsequent autolysis processes. Three key variables were assessed: UV-C exposure time, pH and temperature. The experiments involved the homogenization of intestines in cold distilled water followed by subjecting the mixture into UV-C treatment for exposure times of 0, 10, 20 and 30 minutes. After centrifugation process, the collected supernatant was utilized as the crude enzyme. At various level of temperature (40, 50, 60°C) and pH (6, 7, 8) the activity of crude enzyme was assayed with casein as substrate. The findings revealed the increasing of EA as the UV-C exposure time decreased and pH increased, while the highest EA value was observed at temperature of 50°C. Consequently, the optimal conditions were identified as follows: 0 minutes of UV-C exposure time, pH of 8, and temperature of 50°C. Furthermore, UV-Vis absorption, FTIR, and fluorescence spectroscopy of the crude enzyme after UV-C irradiation induction was studied to investigate the its conformational changes. These additional analyses provided valuable insights into the structural alterations of the crude enzyme caused by the UV-C treatment.

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Section: Enzyme Activity Of Crude Enzymementioning

confidence: 60%

Ultraviolet irradiation effect on characteristics of protease enzyme from African catfish (Clarias sp.) intestines

Tamaya,

Suwanto,

Hernawan

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…To prepare the starter culture of Bacillus licheniformis and Acinetobacter pittii , they were inoculated into sterile nutritional broth and incubated at 37°C with constant shaking at 200 rpm for 24 h. For the enzyme production, a production media containing 30 g of deoiled pongamia meal and 10 mL of nutrient broth was sterilized, and 1 mL of the inoculum (Bacteria: 1 × 10 8 cells/mL and Fungi: 1 × 10 6 spores/mL) was seeded onto the substrate aseptically. The bacterial flasks were incubated at 37°C for 48 h, while the fungal culture was incubated at 28°C for 7 days under static conditions 24 …”

Section: Methodsmentioning

confidence: 99%

“…The bacterial flasks were incubated at 37 C for 48 h, while the fungal culture was incubated at 28 C for 7 days under static conditions. 24…”

Section: Preparation Of Starter Culturementioning

confidence: 99%

Production, properties and applications of proteases from pongamia oil seed cakes

Seshagiri¹,

Parthiban²,

Reddy³

2023

J of Applied Polymer Sci

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De-oiled cakes (meal) containing proteins and carbohydrates are inevitably produced during extraction of oil from seeds. These cakes are inexpensive, sustainable and renewable sources but have limited applications. In this present study, de-oiled pongamia seed cake was used as substrate to produce protease by Bacillus licheniformis, Acinetobacter pittii, and Aspergillus niger under different culture conditions such as pH and temperature. Furthermore, the influence of diverse carbon and nitrogen sources on enzyme production was studied. Under the optimized conditions but without any additional inducers, the activity of protease produced by Aspergillus niger was 27.5 UmL À1 , which is 34% higher than the activity obtained using B. licheniformis and about 6.7 times higher compared to that obtained using A. pittii. Addition of nitrogen or carbon source leads to an increase in enzyme activity of up to 240% with urea by Aspergillus niger and 163% with fructose by Acinetobacter pittii. Proteases obtained had optimum production at pH 8 and temperature of 30 and 40 C.The enzyme produced was effective in hydrolysing the gelatin layer from used x-ray films, enabling the film to be recycled and was also able to coagulate milk, suggesting that it may be suitable for food applications.

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“…Microbial proteases have wide ranging applications in several fields, including baking, brewing, detergents, leather making, pharmaceuticals, meat tenderizing, cosmetics, medical diagnosis and so on ( Christensen et al, 2022 ; Reddy et al, 2022 ; Akram et al, 2023 ; Mubeen et al, 2023 ). In addition, with the rapid development of new fields, applications of microbial proteases are expanding to new areas, such feed industries ( Bernardeau et al, 2022 ; Cupi et al, 2022 ), hydrolysis applications to prepare active peptides ( Christensen et al, 2022 ), and environmental protection applications, such as waste treatment and reuse ( Ariaeenejad et al, 2022 ; Asitok et al, 2022 ; Zhai et al, 2022 ).…”

Section: Application Of Microbial Proteasesmentioning

confidence: 99%

Microbial proteases and their applications

Song,

Zhang,

Wang

et al. 2023

Front. Microbiol.

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Proteases (proteinases or peptidases) are a class of hydrolases that cleave peptide chains in proteins. Endopeptidases are a type of protease that hydrolyze the internal peptide bonds of proteins, forming shorter peptides; exopeptidases hydrolyze the terminal peptide bonds from the C-terminal or N-terminal, forming free amino acids. Microbial proteases are a popular instrument in many industrial applications. In this review, the classification, detection, identification, and sources of microbial proteases are systematically introduced, as well as their applications in food, detergents, waste treatment, and biotechnology processes in the industry fields. In addition, recent studies on techniques used to express heterologous microbial proteases are summarized to describe the process of studying proteases. Finally, future developmental trends for microbial proteases are discussed.

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Production, characterization and applications of proteases produced by Bacillus licheniformis, Acinetobacter pittii and Aspergillus niger using neem seed oil cake as the substrate

Cited by 16 publications

References 38 publications

Ultraviolet irradiation effect on characteristics of protease enzyme from African catfish (Clarias sp.) intestines

Ultraviolet irradiation effect on characteristics of protease enzyme from African catfish (Clarias sp.) intestines

Production, properties and applications of proteases from pongamia oil seed cakes

Microbial proteases and their applications

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