Production, characterization, and in vitro effects of a novel monoclonal antibody against Mig-7

Tapaneeyakorn, Satita; Chantima, Warangkana; Thepthai, Charin; Dharakul, Tararaj

doi:10.1016/j.bbrc.2016.05.062

Cited by 3 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…VE-cadherin was downregulated significantly on both RNA and protein levels. Clinical use of novel, targeted therapies including angiogenesis inhibitors has revealed new features of malignant ovarian tumors and important cancer molecular pathways checkpoints like MIG-7 activation that could be blocked with monoclonal antibodies [30].…”

Section: Discussionmentioning

confidence: 99%

Mig-7 expression and vasculogenic mimicry in malignant ovarian tumors

Czekierdowski

Czekierdowska

Stachowicz³

et al. 2017

Ginekol Pol

View full text Add to dashboard Cite

Objectives:To investigate the possible association of vasculogenic mimicry (VM), VE-cadherin and MIG-7 expression with clinicopathological features of women with malignant ovarian masses. Material and methods:VM was studied with the PAS reaction and VE-cadherin was assessed with immunohistochemistry in 108 women with malignant ovarian tumors. Additionally, quantitative expression of MIG-7 mRNA was performed in 52 ovarian cancers with qRT-PCR.Results: VM was found in 48/108 cases (44%), more often in higher FIGO stage tumors (83% cases; 40 vs. 8; p = 0.01). High expression of VE-cadherin was present in 37% of all ovarian masses. Ovarian tumors without VM more often expressed low levels of VE-cadherin than tumors where VM was found (37.6% vs.14.6%). No expression or very low expression of MIG-7 mRNA was found in all normal ovarian tissues and in 32 cancer samples. Median RQ of MIG-7 mRNA in tumor samples was higher than in normal ovarian tissue (RQ = 0.29 vs. RQ = 0.05, respectively; p < 0.005) and higher than in non-malignant ovarian masses (0.98 vs. 0.05 respectively; p = 0.03). Expression of MIG-7 mRNA was significantly correlated with VM (p = 0.039). In tumors with PAS-positive structures median RQ MIG-7 mRNA was higher than in tumors with PAS-negative findings (1.89 vs. 0.13 respectively). VE-cadherin expression was more frequently found in tumors where MIG-7 mRNA was present (p = 0.004). Conclusions:Vasculogenic mimicry exists in malignant ovarian tumors and advanced clinical stages of malignancy are accompanied by a high incidence of VM formation. MIG-7 mRNA and VE-cadherin expression may serve as additional molecular markers of VM in ovarian malignancies.

show abstract

Section: Discussionmentioning

confidence: 99%

Mig-7 expression and vasculogenic mimicry in malignant ovarian tumors

Czekierdowski

Czekierdowska

Stachowicz³

et al. 2017

Ginekol Pol

View full text Add to dashboard Cite

show abstract

“…Mig-7 significantly affects tumor migration and invasion in hepatocellular carcinoma and osteosarcoma, while it is essential for proliferation and invasion in ovarian cancer [8,9]. Further, Mig-7 is overexpressed in highly invasive tumors but not in non-invasive malignant cells [10][11][12][13], suggesting an important role in tumor invasion. Additionally, knocking down Mig-7 attenuates the invasiveness of U87MG cells [12].…”

mentioning

confidence: 99%

Migration-inducing gene-7 promotes glioma cell proliferation and invasiveness via activating the MAPK signaling pathway

Pan,

Chen,

Huang

et al. 2023

neo

View full text Add to dashboard Cite

Glioma is a highly aggressive primary malignant tumor. Migration-inducing gene-7 (Mig-7) is closely related to tumor invasion and metastasis. However, the detailed molecular mechanism of Mig-7-mediated promotion of glioma cell invasion requires further investigation. Therefore, this study aimed to investigate the molecular mechanism by which Mig-7 promotes invasion and growth of glioma tumor cells. After collecting 65 glioma tissues and 16 non-tumor tissues, the expression difference of Mig-7 between tumor tissues and non-tumor tissues was analyzed. The molecular mechanism of Mig-7 in tumor cells was investigated by knockdown or overexpression of Mig-7 in U87MG cells. Specifically, the expression levels of mitogen-activated protein kinase (MAPK) signaling pathway-related molecules were detected in cells that knocked down Mig-7. MTT, Transwell, and three-dimensional cell culture assays were used to detect the survival, migration, invasion, and tube formation of U87MG cells that overexpressed Mig-7 were treated with the MAPK signaling pathway inhibitors (SP600125, SCH772984, and SB202190). The effect of Mig-7 on the tumorigenic ability of U87MG cells was investigated by subcutaneous tumorigenic experiment in nude mice. The corresponding results indicated that Mig-7 expression was significantly higher in glioma tissues and cell lines compared to that in non-neoplastic brain tissues and normal glial cell lines. In U87MG cells, downregulation or overexpression of Mig-7 inhibited or promoted the expression of MMP-2, MMP-9, LAMC2, EphA2, and VE-cadherin, and phosphorylation levels of ERK1/2, JNK, and p38. Mig-7 overexpression promoted migration, invasion, cell viability, and tube formation, which were reversed by the MAPK signaling pathway inhibitors. Mig-7 overexpression promoted subcutaneous tumor growth in mice and upregulated the phosphorylation levels of ERK1/2, JNK, and p38 and the expression of Ki-67. These effects of Mig-7 overexpression were reversed by MAPK pathway inhibitors. Overall, these results suggest that Mig-7 may be a novel biomarker and potential therapeutic target for glioma, with the MAPK pathway playing a key role in the corresponding Mig-7 mechanism of action.

show abstract

Biomarkerless targeting and photothermal cancer cell killing by surface-electrically-charged superparamagnetic Fe₃O₄composite nanoparticles

et al. 2017

View full text Add to dashboard Cite

A major challenge in cancer therapy is localized targeting of cancer cells for maximum therapeutic effectiveness. However, due to cancer heterogeneities, the biomarkers are either not readily available or specific for effective targeting of cancer cells. The key, therefore, is to develop a new targeting strategy that does not rely on biomarkers. A general hallmark of cancer cells is the much increased level of glycolysis. The loss of highly mobile lactate from the cytoplasm inevitably removes labile inorganic cations to form lactate salts and acids as part of the lactate cycle, creating a net of negative surface charges. This net of negative charges on cancer cell surfaces biophysically distinguishes themselves from normal cells. In this study, cancer cells are targeted by using positively-charged, fluorescent, superparamagnetic FeO-composite nanoparticles. The positively-charged FeO composite nanoparticles bind predominantly to cancer cells due to their negatively-charged surfaces. Upon electrical-charge-mediated FeO nanoparticle binding onto cancer cells, irradiation by using an 808 nm laser is subsequently applied to induce photothermal hyperthermia that kills the cancer cells directly. The negatively-charged composite nanoparticles are found, however, not to target and bind the cancer cells due to the electrostatic repulsive force between them. This unique strategy paves a new path for effective targeting and direct cancer cell killing without relying on any biomarkers and anticancer drugs.

show abstract

Production, characterization, and in vitro effects of a novel monoclonal antibody against Mig-7

Cited by 3 publications

References 14 publications

Mig-7 expression and vasculogenic mimicry in malignant ovarian tumors

Mig-7 expression and vasculogenic mimicry in malignant ovarian tumors

Migration-inducing gene-7 promotes glioma cell proliferation and invasiveness via activating the MAPK signaling pathway

Biomarkerless targeting and photothermal cancer cell killing by surface-electrically-charged superparamagnetic Fe₃O₄composite nanoparticles

Contact Info

Product

Resources

About

Production, characterization, and in vitro effects of a novel monoclonal antibody against Mig-7

Cited by 3 publications

References 14 publications

Mig-7 expression and vasculogenic mimicry in malignant ovarian tumors

Mig-7 expression and vasculogenic mimicry in malignant ovarian tumors

Migration-inducing gene-7 promotes glioma cell proliferation and invasiveness via activating the MAPK signaling pathway

Biomarkerless targeting and photothermal cancer cell killing by surface-electrically-charged superparamagnetic Fe3O4composite nanoparticles

Contact Info

Product

Resources

About

Biomarkerless targeting and photothermal cancer cell killing by surface-electrically-charged superparamagnetic Fe₃O₄composite nanoparticles