The aim of this study was to estimate the effect of variation in the fatty
acid binding protein 4 gene (FABP4) on milk production traits in Greek Sfakia
sheep. Polymerase chain reaction – single-stranded conformational
polymorphism (PCR-SSCP) analysis was used to genotype a total of 374 Sfakia
ewes for two regions of FABP4 located around exon 2–intron 2 (Region 1) and
exon 3–intron 3 (Region 2). Each month, for a period of 6 months, milk
samples were collected from the ewes to measure total milk yield, fat
content, protein content, lactose content, non-fat solid content, pH, and
somatic cell count (SCC). A general linear model was used to test the
association between the variation observed in FABP4 and milk production traits.
Four gene variants (A1–A4) were found in Region 1 and two variants
(C1–C2) were found in Region 2. In the first region, the FABP4 genotype
significantly affected (P<0.05) non-fat solid levels, fat content,
and SCC. The presence of the A2 variant was significantly associated (P<0.05)
with decreased SCC, while the presence of A4 was significantly associated with
decreased milk yield (P<0.01), increased non-fat solid content (P<0.05),
decreased fat content (P<0.01), increased lactose content (P<0.05), and
increased pH (P<0.05). In the second region, FABP4 genotype had an effect (P<0.05) on protein content and the presence of the C2 variant was
associated (P<0.05) with increased protein content, decreased SCC, and lower
pH. The results suggest an association between variation in ovine FABP4 and milk
production traits in Greek Sfakia sheep. Nevertheless, further analyses in
independent sheep populations of increased size will strengthen these
findings.