Production de vitroplants de palmier dattier à l’échelle pilote

Fki, Lotfi; Kiaa, W.; Sahnoun, N.; Bouaziz, Neila; Masmoudi, R.; Drira, Noureddine

doi:10.4000/books.irdeditions.10779

Biotechnologies Du Palmier Dattier 2010

DOI: 10.4000/books.irdeditions.10779

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Production de vitroplants de palmier dattier à l’échelle pilote

Lotfi Fki¹,

W. Kiaa²,

N. Sahnoun³

et al.

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2017

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In Vitro Cryopreservation of Date Palm Caulogenic Meristems

Fki

Chkir

Kriaâ

et al. 2017

Methods in Molecular Biology

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Cryopreservation is the technology of choice not only for plant genetic resource preservation but also for virus eradication and for the efficient management of large-scale micropropagation. In this chapter, we describe three cryopreservation protocols (standard vitrification, droplet vitrification, and encapsulation vitrification) for date palm highly proliferating meristems that are initiated from vitro-cultures using plant growth regulator-free MS medium. The positive impact of sucrose preculture and cold hardening treatments on survival rates is significant. Regeneration rates obtained with standard vitrification, encapsulationvitrification, and droplet-vitrification protocols can reach 30, 40, and 70%, respectively. All regenerated plants from non-cryopreserved or cryopreserved explants don't show morphological variation by maintaining genetic integrity without adverse effect of cryogenic treatment. Cryopreservation of date palm vitro-cultures enables commercial tissue culture laboratories to move to large-scale propagation from cryopreserved cell lines producing true-to-type plants after clonal field-testing trials. When comparing the cost of cryostorage and in-field conservation of date palm cultivars, tissue cryopreservation is the most cost-effective. Moreover, many of the risks linked to field conservation like erosion due to climatic, edaphic, and phytopathologic constraints are circumvented.

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In Vitro Cryopreservation of Date Palm Caulogenic Meristems

Fki

Chkir

Kriaâ

et al. 2017

Methods in Molecular Biology

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Production de vitroplants de palmier dattier à l’échelle pilote

Cited by 1 publication

References 24 publications

In Vitro Cryopreservation of Date Palm Caulogenic Meristems

In Vitro Cryopreservation of Date Palm Caulogenic Meristems

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