Production enhancement and characterization of the polyhydroxyalkanoate produced by Natrinema ajinwuensis (as synonym) ≡ Natrinema altunense strain RM-G10

Mahansaria, Riddhi; Dhara, Anusua; Saha, Amit; Haldar, Sumanta; Mukherjee, Joydeep

doi:10.1016/j.ijbiomac.2017.10.009

Cited by 35 publications

(15 citation statements)

References 49 publications

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“…Han and his colleagues showed a maximum yield in a shorter time (96 h of fermentation) compared to the time required by isolate CEJGTEA101. Additionally, it was notable that Natrinema ajinwuensis RM-G10 accumulated about 61% PHBV of its CDW [29]. These results showed that PHA content and its composition depend on species, growth conditions and type of substrate utilized.…”

Section: Discussionmentioning

confidence: 78%

“…Similarly, Haloarcula japonica strain T5 accumulated 1 wt % PHB of dried cells when growing on molasses, a by-product of sugar manufacturing industries [12]. Besides the use of waste products, glucose is currently being utilized by Haloarchaea for PHA accumulation except Halobacterium, Natrialba, and Natronomonas strains [29]. The isolate CEJGTEA101 could consume both starch and glucose as sole carbon source, however, utilization of glucose was more rapid than starch.…”

Section: Discussionmentioning

confidence: 99%

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Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources

2020

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The large use of conventional plastics has resulted in serious environmental problems. Polyhydroxyalkanoates represent a potent replacement to synthetic plastics because of their biodegradable nature. This study aimed to screen bacteria and archaea isolated from an extreme environment, the salt lake Chott El Jerid for the accumulation of these inclusions. Among them, two archaeal strains showed positive results with phenotypic and genotypic methods. Phylogenetic analysis, based on the 16S rRNA gene, indicated that polyhydroxyalkanoate (PHA)-producing archaeal isolates CEJGTEA101 and CEJEA36 were related to Natrinema altunense and Haloterrigena jeotgali, respectively. Gas chromatography and UV-visible spectrophotometric analyses revealed that the PHA were identified as polyhydroxybutyrate and polyhydroxyvalerate, respectively. According to gas chromatography analysis, the strain CEJGTEA101 produced maximum yield of 7 wt % at 37 °C; pH 6.5; 20% NaCl and the strain CEJEA36 produced 3.6 wt % at 37 °C; pH 7; 25% NaCl in a medium supplemented with 2% glucose. Under nutritionally optimal cultivation conditions, polymers were extracted from these strains and were determined by gravimetric analysis yielding PHA production of 35% and 25% of cell dry weight. In conclusion, optimization of PHA production from inexpensive industrial wastes and carbon sources has considerable interest for reducing costs and obtaining high yield.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources

2020

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“…hispanica, Halogeometricum borinquense, and Natrinema species were also found to accumulate PHBV only by utilizing unrelated carbon sources [41][42][43][44]. For example, Natrinema ajinwuensis accumulated PHBV containing 13.93 mol% 3HV by using glucose as the sole carbon source [45]. Halogranum amylolyticum could also efficiently accumulate PHBV with 20.1 mol% 3HV from glucose [46].…”

Section: Haloarchaea As Pha Producersmentioning

confidence: 99%

Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory

et al. 2020

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Plastic pollution is a severe threat to our environment which necessitates implementation of bioplastics to realize sustainable development for a green world. Polyhydroxyalkanoates (PHA) represent one of the potential candidates for these bioplastics. However, a major challenge faced by PHA is the high production cost which limits its commercial application. Halophiles are considered to be a promising cell factory for PHA synthesis due to its several unique characteristics including high salinity requirement preventing microbial contamination, high intracellular osmotic pressure allowing easy cell lysis for PHA recovery, and capability to utilize wide spectrum of low-cost substrates. Optimization of fermentation parameters has made it plausible to achieve large-scale production at low cost by using halophiles. Further deeper insights into halophiles have revealed the existence of diversified and even novel PHA synthetic pathways within different halophilic species that greatly affects PHA type. Thus, precise metabolic engineering of halophiles with the help of advanced tools and strategies have led to more efficient microbial cell factory for PHA production. This review is an endeavour to summarize the various research achievements in these areas which will help the readers to understand the current developments as well as the future efforts in PHA research.

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“…PHA accumulation by haloarchaea was first reported during the characterization of halophilic strains in 1972 . Then Haloarcula marismortui ( H. marismortui ), Haloferax mediterranei ( H. mediterranei ), Haloquadratum walsbyi , H. marismortui , Halogeometricum borinquense ( H. borinquense ), and Natrinema altunense ( N. altunense ) were all reported to produce PHA, including PHB and PHBV . Among the halophilic archaea, H. mediterranei accumulates PHA up to 67% of the CDW utilizing starch or glucose as a carbon substrate, under batch cultures and phosphorous limitation at a higher temperature .…”

Section: Halophilic Chassis For Bioproductionmentioning

confidence: 99%

“…N. altunense accumulated 61.02 wt% PHBV consisting of 13.93 mol% 3HV at 72 h, with its volumetric productivity reaching 0.210 g L −1 h −1 . H. mediterranei synthesized a terpolymer poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyvalerate‐ co ‐4‐hydroxybutyrate) reaching 87.5% in cell dry weight when the medium was supplemented with a mixed carbon source of hydrolyzed whey, γ‐butyrolactone, and valerate .…”

Section: Halophilic Chassis For Bioproductionmentioning

confidence: 99%

Halophiles as Chassis for Bioproduction

2018

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Industrial biotechnology aims to solve the challenges of petroleum‐based production using microorganisms as catalysts. However, current industrial biotechnology suffers from high energy and fresh water consumption as well as difficult downstream purification. In addition, most industrial microorganisms are not resistant to other microbial contaminations, and this seriously compromises process effectiveness and the economy. The recently proposed “next‐generation industrial biotechnology” employs contamination resistant microorganisms, including alkaliphilic, acidophilic, thermophilic or halophilic bacteria, etc., for bioproduction. The most successful example is the use of halophilic bacteria as chassis for the production of chemicals, proteins, and biopolymers. This review summarizes some of the most recent studies to engineer the halophilic chassis Halomonas spp. for the production of chemicals, proteins, and several biopolyesters such as poly(3‐hydroxybutyrate), copolymers of 3‐hydroxybutyrate and 3‐hydroxyvalerate, and copolymers of 3‐hydroxybutyrate and 4‐hydroxybutyrate. The Halomonas chassis is also successfully engineered to change its morphology from short bars to long fibers and large spheres for convenient gravity separation. This simplifies the bioprocessing.

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Production enhancement and characterization of the polyhydroxyalkanoate produced by Natrinema ajinwuensis (as synonym) ≡ Natrinema altunense strain RM-G10

Cited by 35 publications

References 49 publications

Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources

Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources

Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory

Halophiles as Chassis for Bioproduction

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