Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain

Abstract

The genus Leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides (EPS). These biopolymers possess considerable commercial potential. Because of the wide variety of industrial applications of EPS, this study aimed to produce and characterize the native exopolysaccharide strain Leuconostoc pseudomesenteroides R2, which was isolated from cabbage collected in a semi-arid region of Bahia. We employed the following conditions for the production of EPS: 10.7% sucrose, pH 8.2, without agitation and incubation at 28°C for 30 hours. The fermentation broth was treated with ethanol and generated two types of polysaccharide substances (EPS I and EPS II). The identification of EPS I and EPS II was conducted using FT-IR, 1 H, 13 C and DEPT-135 NMR spectra. The two substances were identified as linear dextran α polysaccharides (1 → 6) which indicated different characteristics with respect to thermal analysis and density of free packaging, viscosity and time of solubilization. Both dextrans are of low density, possess high thermal stability and exhibited the behavior characteristic of pseudoplastic polymers.

Structure and Biosynthesis of Two Exopolysaccharides Produced by Lactobacillus johnsonii FI9785

“…The L. johnsonii FI9785 genome does not contain any annotated genes with clear homology to glucansucrases. The production of more than one EPS has also been demonstrated in other lactic acid bacteria; Lactobacillus plantarum EP56 expressed two heteropolysaccharides, one cell-bound and one released (32), whereas the two EPS produced by Leuconostoc pseudomesenteroides R2 were both linear dextrans with different characteristics (33). …”

Antioxidant Activities of an Exopolysaccharide (DeinoPol) Produced by the Extreme Radiation-Resistant Bacterium Deinococcus radiodurans

“…To date, most industrially important EPSs are produced in plants, animals, or algae, because of the low cost of downstream processing 10,34,35 . However, the use of plant-and animal-derived resources is becoming limited because of environmental disruption, depletion, and contamination.…”

Potentials of the microalgae inoculant in restoration of biological soil crusts to combat desertification

“…In the primary step which normally happens in the first year, the sandy soil surface may become fixed by the bacteria adhering to the sand particles with exopolysaccharides (Paulo et al 2012). Up to 4 years, the exposed crusts to the sandy surface would mainly be composed of the filamentous cyanobacterial communities that are dominated by the Microcoleus spp., which occurs as a cluster of filaments surrounded by a gelatinous sheath.…”