Production in vitro of toxic macromolecules by strains of Beauveria bassiana, and purification of a chitosanase-like protein secreted by a melanizing isolate

Fuguet, Rosa; Théraud, Magali; Vey, A.

doi:10.1016/j.cca.2004.06.009

Cited by 25 publications

(6 citation statements)

References 22 publications

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“…Some researchers found that the macromolecular protein Bclp was secreted by B. bassiana and could damage the cell and the cuticle of Galleria mellonella (Fuguet et al, 2004b). Furthermore, the toxin isolated from the metabolites of B. bassiana could damage the cell membrane, nucleus, mitochondria and ribosomes of Spodoptera frugiperda (Wu et al, 1999).…”

Section: Function Of the Toxin Produced By B Bassiana Tst05mentioning

confidence: 96%

Morphological and ultrastructural characterization of Carposina sasakii larvae (Lepidoptera: Carposinidae) infected by Beauveria bassiana (Ascomycota: Hypocreales: Clavicipitaceae)

Xiong

Xie

Zhu

et al. 2013

Micron

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Section: Function Of the Toxin Produced By B Bassiana Tst05mentioning

confidence: 96%

Morphological and ultrastructural characterization of Carposina sasakii larvae (Lepidoptera: Carposinidae) infected by Beauveria bassiana (Ascomycota: Hypocreales: Clavicipitaceae)

Xiong

Xie

Zhu

et al. 2013

Micron

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“…The fungal culture was maintained on potato-dextrose agar slants [16], incubated at 28 ± 1 °C for 7 d, then stored at 4 °C until use. Coinidia suspension was harvested by scraping the surface of sporulating slants (7 d old cultures) in 10 ml of 0.85% sterile saline solution [17]. Each ml of suspension contained 10 6 -10 7 spores as counted by haemocytometer.…”

Section: Microorganism and Inoculum Preparationmentioning

confidence: 99%

L‐methioninase production by Aspergillus flavipes under solid‐state fermentation

El-Sayed

2009

J. Basic Microbiol.

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Solid-state fermentation was carried out for the production of extra-cellular L-methioninase by Aspergillus flavipes (Bain and Sart.) using nine agro-industrial residues, namely wheat bran, rice bran, wheat flour, coconut seeds, cotton seeds, ground nut cake, lentil hulls, soya beans and chicken feathers. Chicken feathers were selected as solid substrate for L-methioninase production by A. flavipes. The maximum L-methioninase productivity (71.0 U/mg protein) and growth (11 mg protein/ml) of A. flavipes was obtained using alkali pretreated chicken feathers of 50% initial moisture content as substrate supplemented with D-glucose (1.0% w/v) and L-methionine (0.2% w/v). External supplementation of the fermentation medium with various vitamin sources has no overinductive effect on L-methioninase biosynthesis. The partially purified A. flavipes L-methioninase preparation showed highest activity (181 U/ml) at pH 8.0 with stability over a pH range (pH 6-8) for 2 h. L-methioninase activity was increased by preincubation of the enzyme for 2 h with Co(2+), Mn(2+), Cu(2+) and Mg(2+) and strongly inhibited by the presence of EDTA, NaN(3), Li(2+), Cd(2+), DMSO and 2-mercaptoethanol. The enzyme preparation has a broad substrate spectrum showing a higher affinity to deaminate L-glycine, N -acetylglucosamine and glutamic acid, in addition to their proteolytic activity against bovine serum albumin, casein, gelatin and keratin. The partially purified enzyme was found to be glyco-metalloproteinic in nature as concluded from the analytical and spectroscopic profiles of the enzyme preparation. The demethiolating activity of the enzyme was also visualized chromogenially.

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“…The B. bassiana lifestyle is unusual in that in addition to being a necrotrophic parasite of a broad range of arthropods, it is a facultative saprophyte and can exist as a plant endophyte (Bing & Lewis, 1991). An opportunistic pathogen, B. bassiana does not require any specialized route of entry towards susceptible host targets, and an array of depolymerases (proteases, chitinases) and secondary metabolites participate in the infection process (Charnley & St Leger, 1991;El-Sayed et al, 1993;Fuguet et al, 2004;Kirkland et al, 2005). Different infectious B. bassiana propagules can be isolated and selected for host targeting (Alves et al, 2002;Kirkland et al, 2004b;Thomas et al, 1987;Viaud et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Surface characteristics of the entomopathogenic fungus Beauveria (Cordyceps) bassiana

et al. 2007

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Marked differences in surface characteristics were observed among three types of single-cell propagules produced by the entomopathogenic fungus Beauveria bassiana. Atomic force microscopy (AFM) revealed the presence of bundles or fascicles in aerial conidia absent from in vitro blastospores and submerged conidia. Contact angle measurements using polar and apolar test liquids placed on cell layers were used to calculate surface tension values and the free energies of interaction of the cell types with surfaces. These analyses indicated that the cell surfaces of aerial conidia were hydrophobic, whereas those of blastospores and submerged conidia were hydrophilic. Zeta potential determinations of the electrostatic charge distribution across the surface of the cells varied from +22 to "30 mV for 16-day aerial conidia at pH values ranging from 3 to 9, while the net surface charge ranged from +10 to "13 mV for submerged conidia, with much less variation observed for blastospores, +4 to "4 mV, over the same pH range. Measurements of hydrophobicity using microbial adhesion to hydrocarbons (MATH) indicated that the surfaces of aerial conidia were hydrophobic, and those of blastospores hydrophilic, whereas submerged conidia displayed cell surface characteristics on the borderline between hydrophobic and hydrophilic. Insect pathology assays using tobacco budworm (Heliothis virescens) larvae revealed some variation in virulence among aerial conidia, in vitro blastospores and submerged conidia, using both topical application and haemocoel injection of the fungal cells.

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Production in vitro of toxic macromolecules by strains of Beauveria bassiana, and purification of a chitosanase-like protein secreted by a melanizing isolate

Cited by 25 publications

References 22 publications

Morphological and ultrastructural characterization of Carposina sasakii larvae (Lepidoptera: Carposinidae) infected by Beauveria bassiana (Ascomycota: Hypocreales: Clavicipitaceae)

Morphological and ultrastructural characterization of Carposina sasakii larvae (Lepidoptera: Carposinidae) infected by Beauveria bassiana (Ascomycota: Hypocreales: Clavicipitaceae)

L‐methioninase production by Aspergillus flavipes under solid‐state fermentation

Surface characteristics of the entomopathogenic fungus Beauveria (Cordyceps) bassiana

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