Production, isolation and partial purification of xylanases from anAspergillus sp.

Khanna, P. K.; Ss, Sundari; Nj, Kumar

doi:10.1007/bf00704661

Cited by 91 publications

(92 citation statements)

References 4 publications

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“…The production of extracellular tannases by the selected fungal strains was analyzed using Czapeck [16], Khanna [17], M5 [18], Mineral Medium (MM) [19] and SR [20] media, containing 1% (w/v) tannic acid as additional or as the only carbon source, with initial pH adjusted to 5.0. Cultures were maintained under both orbital agitation at 120 rpm and stationary conditions, at 37°C, for different periods according to the best cultivation period for each specie previously determined.…”

Section: Effect Of Different Culture Mediamentioning

confidence: 99%

Screening, Selection and Optimization of the Culture Conditions for Tannase Production by Endophytic Fungi Isolated from Caatinga

Cavalcanti¹,

Ornela²,

Jorge³

et al. 2017

J App Biol Biotech

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Tannin acyl hydrolase (EC 3.1.1.20), called tannase, is an enzyme of great biotechnological interest for applications in food, chemical, beverage and pharmaceutical industries. Therefore, the objective of this study was to isolate, select and identify strains of endophytic fungi from rich tannin plants collected in the Caatinga, as well as, the optimization of culture conditions for the best producers. Sixteen endophyte fungi were isolated from the barks of mastic (Myracrodruon urundeuva Allemão), angico (Anadenanthera colubrina Vell.), barauna (Schinopsis brasiliensis Engl.), cajueiro (Anacardium occidentale L.) and catingueira (Caesalpinia pyramidalis Tul). All strains showed ability of using tannic acid as carbon source. The species A. niger and A. fumigatus isolated from angico and cajueiro, respectively, presented the highest enzyme production. The optimum conditions for the production of tannase by A. niger were 24 h cultivation in Khanna medium containing 2% tannic acid, in the absence of nitrogen source, at 37 °C. A. fumigatus showed increased production of tannase when cultured in mineral medium for 24 h using 2% tannic acid as carbon source and peptone as additional nitrogen source, at 37 ºC. The optimum apparent temperature and pH of activity for the enzymes produced by both fungal species were 30 ºC and 4.0, respectively.

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Section: Effect Of Different Culture Mediamentioning

confidence: 99%

Screening, Selection and Optimization of the Culture Conditions for Tannase Production by Endophytic Fungi Isolated from Caatinga

Cavalcanti¹,

Ornela²,

Jorge³

et al. 2017

J App Biol Biotech

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“…The cultures were carried out in 125 mL Erlenmeyer flasks containing 25 mL of one of the following liquid media: AM (Adams 1990, Peixoto et al 2003; Khanna (Khanna et al 1995), Segato Rizzatti (SR, Rizzatti et al 2001) or Vogel (Vogel 1964). The pH of the medium was adjusted to 6.5 and it was supplemented with 1% soluble starch, as carbon source.…”

Section: Optimization Of Culturementioning

confidence: 99%

Bioprospection and characterization of the amylolytic activity by filamentous fungi from Brazilian Atlantic Forest

et al. 2017

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Filamentous fungi are widely diverse and ubiquitous organisms. Such biodiversity is barely known, making room for a great potential still to be discovered, especially in tropical environments -which are favorable to growth and species variety. Filamentous fungi are extensively applied to the production of industrial enzymes, such as the amylases. This class of enzymes acts in the hydrolysis of starch to glucose or maltooligosaccharides. In this work twenty-five filamentous fungi were isolated from samples of decomposing material collected in the Brazilian Atlantic Forest. The two best amylase producers were identified as Aspergillus brasiliensis and Rhizopus oryzae. Both are mesophilic, they grow well in organic nitrogen-rich media produce great amounts of glucoamylases. The enzymes of A. brasiliensis and R. oryzae are different, possibly because of their phylogenetical distance. The best amylase production of A. brasiliensis occurred during 120 hours with initial pH of 7.5; it had a better activity in the pH range of 3.5-5.0 and at 60-75°C. Both fungal glucoamylase had wide pH stability (3-8) and were activated by Mn 2+. R. oryzae best production occurred in 96 hours and at pH 6.5. Its amylases had a greater activity in the pH range of 4.0-5.5 and temperature at 50-65ºC. The most significant difference between the enzymes produced by both fungi is the resistance to thermal denaturation: A. brasiliensis glucoamylase had a T 50 of 60 minutes at 70ºC. The R. oryzae glucoamylase only had a residual activity when incubated at 50°C with a 12 min T 50 . Keywords: Amylase, Filamentous fungi, Aspergillus brasiliensis, Rhizopus oryzae, Glucoamylase, Bioprospection Bioprospecção e caracterização da atividade amilolítica de fungos filamentosos da Mata Atlântica BrasileiraResumo: Fungos filamentosos são organismos amplamente diversificados e ubíquos. Esta biodiversidade ainda é pouco caracterizada, desta forma, há um grande potencial a ser descoberto, sobretudo em biomas tropicais, que favorecem o crescimento e diversificação de espécies. Fungos filamentosos são extensivamente utilizados para a produção industrial de enzimas, como as amilases. Esta classe de enzimas atua na hidrólise do amido em glicose ou maltooligossacarídeos. Neste trabalho 25 cepas de fungos filamentosos foram isoladas a partir de amostras de material em decomposição coletados na Mata Atlântica Brasileira. As duas cepas que produziram mais amilases foram identificadas como Aspergillus brasiliensis e Rhizopus oryzae. Ambos os fungos são mesofílicos, crescem bem em meio de cultivo rico em nitrogênio orgânico, e produziram grande quantidade de glucoamilase. As enzimas de A. brasiliensis e R. oryzae possuem características distintas, possivelmente devido à distância filogenética das espécies. A produção de amilase mais expressiva de A. brasiliensis ocorreu em 120 horas de cultivo e pH inicial de 7,5; possui maior atividade em temperaturas entre 60-75ºC e pH entre 3,5-5,0. Ambas glucoamilases fúngicas obtiveram ampla estabilidade de pH (3-8) e fora...

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“…Cultures were carried out in Erlenmeyer flasks (150 mL) containing 1% soy oil and 25 mL of liquid media: SR (RIZZATTI et al, 2001); Khanna (KHANNA et al, 1995); Adams (ADAMS, 1990) or Czapek (WISEMAN, 1975), at 30°C, 100 rpm. Aliquots were taken each 24h and the lipase activity analyzed.…”

Section: Optimization Of Fermentation Parameters On Fusarium Oxysporumentioning

confidence: 99%

A Useful Methodology to Select Lipase-Catalyzed Transesterification Aiming Biodiesel Application

Facchini

Vici

Pereira

et al. 2016

Revista Brasileira De Engenharia De Biossistemas

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The application of lipases in various fields has been notably increased in the last few decades and qualitative/quantitative improvements need to be done. However, many methodologies of screening are described in order to find a good lipase producer and statistical optimization is a necessary tool to improve lipase production. In this work, an isolation of filamentous fungi lipase producers and a transesterification capacity screening was evaluated. Four fungi were chosen to the transesterification reaction assays and the best fungus selected was submitted to a submerged fermentation. Parameters of the culture medium were optimized using response surface methodology. Selected liquid medium was SR at 30 °C, 72 h, 100 rpm. Corn oil was the best carbon source and together with Tween 80 increased two-fold the lipase activity. After the experimental design, the new medium optimized were 3.5-fold higher than the original liquid medium and was composed by 0.5% corn oil, 0.012% MgSO 4 .7H 2 O, 0.015% KH 2 PO 4 , 0.05% NH 4 H 2 PO 4 . Hence, the lipase produced proved its transesterification capacity and can be used for biodiesel production. Keywords: Lipase; Transesterification; Biodiesel UMA METODOLOGIA ÚTIL PARA SELECIONAR LIPASES COM ATIVIDADE DE TRANSESTERIFICAÇÃO VISANDO APLICAÇÃO NA PRODUÇÃO DE BIODIESEL RESUMOA aplicação de lipases em vários campos tem aumentado notavelmente nas últimas décadas e melhorias qualitativas/quantitativas precisam ser feitas. Entretanto, a fim de encontrar um bom produtor de lipase muitas metodologias de seleção são descritas, além disso, a otimização estatística é uma ferramenta necessária para melhorar a produção de lipases. Este trabalho apresenta o isolamento de fungos filamentosos produtores de lipase e a avaliação da capacidade de transesterificação dessas enzimas. Quatro fungos foram selecionados para os ensaios de transesterificação e o melhor fungo isolado foi submetido a fermentação submersa. Parâmetros do meio de cultivo foram otimizados usando metodologia de superfície de resposta. O meio de cultivo líquido selecionado foi o SR, a 30 °C, 72 h e 100 rpm. Óleo de milho foi a melhor fonte de carbono e, quando adicionado de Tween 80 aumentou duas vezes a atividade de lipase. Após o planejamento experimental, o novo meio de cultivo otimizado, composto por * polizeli@ffclrp.usp.br Brazilian Journal of Biosystems Engineering v. 10(1): 01-13, 2016 2 óleo de milho 0,5%, extrato de levedura 0,45%, peptona 0,2%, MgSO 4 .7H 2 O 0,012%, KH 2 PO 4 0,015% e NH 4 H 2 PO 4 0,05%, foi 3,5 vezes melhor que o original. Dessa forma, a lipase produzida demonstrou capacidade de transesterificação e pode ser utilizada para a produção de biodiesel.

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Production, isolation and partial purification of xylanases from anAspergillus sp.

Cited by 91 publications

References 4 publications

Screening, Selection and Optimization of the Culture Conditions for Tannase Production by Endophytic Fungi Isolated from Caatinga

Screening, Selection and Optimization of the Culture Conditions for Tannase Production by Endophytic Fungi Isolated from Caatinga

Bioprospection and characterization of the amylolytic activity by filamentous fungi from Brazilian Atlantic Forest

A Useful Methodology to Select Lipase-Catalyzed Transesterification Aiming Biodiesel Application

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