Production of 2-butanol throughmeso-2,3-butanediol consumption in lactic acid bacteria

Abstract

2-Butanol has been an issue of industries in many areas, for example, biofuel production (as an advanced alternate fuel), fermented beverages, and food (as taste-altering component). Thus, its source of production, the biological pathway, and the enzymes involved are of high interest. In this study, 42 different isolates of lactic acid bacteria from nine different species were screened for their capability to consume meso-2,3-butanediol and produce 2-butanol. Lactobacillus brevis was the only species that showed any production of 2-butanol. Five of ten tested isolates of L. brevis were able to convert meso-2,3-butanediol to 2-butanol in a synthetic medium (SM2). However, none of them showed the same capability in a complex medium such as MRS indicating that the ability to produce 2-butanol is subject to some kind of repression mechanism. Furthermore, by evaluating the performance of the enzymes required to convert meso-2,3-butanediol to 2-butanol, that is, the secondary alcohol dehydrogenase and the diol dehydratase, it was shown that the latter needed the presence of a substrate to be expressed.

Identification of fungal metabolites from inside Gallus gallus domesticus eggshells by non-invasively detecting volatile organic compounds (VOCs)

“…From those, only 2 were identified (Table 1): 2-butanol and N,N-dimethylformamide. Even though the 2-Butanol compound is reported in the mVOC database as produced by fungi, it has also been found to be produced by lactic acid bacteria (46). N,N-dimethylformamide is released by Paenibacillus polymyxa (47), a bacterium found in the soil, roots, and rhizosphere of various crop plants.…”

Synergy at work: linking the metabolism of two lactic acid bacteria to achieve superior production of 2-butanol

“…1b). As a source of the diol dehydratase we chose L. brevis SE20, which previously has been shown to produce 2-butanol when supplied with mBDO and vitamin B12 [35]. Our hypothesis was that the mBDO formed in L. lactis would leave the cells and enter the L. brevis cells to be dehydrated into 2-butanone.…”

“…Lactobacillus brevis SE20 [35], isolated from an ethanol pilot plant facility in Örnsköldsvik Sweden was kindly provided by Christer Larsson (Chalmers University of Technology, Sweden). Escherichia coli strain Top10 {F-mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacΧ74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (StrR) endA1 nupG λ-} was used for cloning purposes.…”

Waste Degradation and Utilization by Lactic Acid Bacteria: Use of Lactic Acid Bacteria in Production of Food Additives, Bioenergy and Biogas

“…These strains converted meso-2,3-butanediol to 2-butanol in a synthetic medium, but none of them showed the same ability in a complex medium such as MRS. It appears that the process is inhibited by some kind of repression mechanism [200].…”