Production of 3′,5′-cyclic diadenosine monophosphate complex with Mg,Al-layered double hydroxide

Abstract

The possibility of using Mg,Al-layered double hydroxide (LDH) for production of nanoparticles comprising molecules of pharmaceutically valuable 3′,5′-cyclic diadenosine monophosphate (cyclic di-AMP) was demonstrated. Experimental conditions were optimized to produce LDH complexes with cyclic di-AMP (average size of 300 nm), which loading capacity in regard to the target compound reached 60 mass %. The fact of cyclic di-AMP release from LDH complex in pH-dependent mode (proceeding slightly slower at pH 7.4 than at pH 4.5) was stated. The obtained results testify in favor of applying the studied nanocomplexes for delayed delivery of cyclic di-AMP to the target immune cells.