Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process

Joo, Jeong Chan; Oh, Young Hoon; Yu, Ji Hea; Hyun, Sung Min; Khang, Tae Uk; Kang, Kyoung Hee; Song, Bong Keun; Park, Kyungmoon; Oh, Min Kyu; Lee, Sang Yup; Park, Si Jae

doi:10.1016/j.biortech.2017.05.131

Cited by 50 publications

(42 citation statements)

References 31 publications

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“…5‐Aminovalerate production from alternative carbon sources, such as d ‐xylose and l ‐arabinose, was also demonstrated by additional heterologous expression of xylA from X. campestris and the araBAD operon from E. coli . Using biosugars from a Miscanthus hydrolysate, 33.93 g L −1 5‐aminovalerate was produced by the fed‐batch cultivation of recombinant C. glutamicum 1857 expressing codon‐optimized davBA genes under a strong H30 promoter (Table ) …”

Section: Production Of Polyamide Monomersmentioning

confidence: 99%

“…Although C. glutamicum exhibits weak carbon catabolite repression toward mixed carbon sources, it cannot directly utilize complex polysaccharide polymers, such as xylan, cellulose, and starch, from lignocellulosic and starch biomass. To increase the economic feasibility of the biorefinery process, C. glutamicum has been engineered for the consolidated bioprocessing (CBP) of microalgal biomass and hemicellulose by the heterologous expression of amylolytic and cellulolytic enzymes . These recombinant C. glutamicum strains simultaneously hydrolyze biomass and produce biochemicals such as succinate, l ‐lysine, and xylonic acid using released glucose, maltose, cellobiose, d ‐xylose, and l ‐arabinose from biomass (Fig.…”

Section: Introductionmentioning

confidence: 99%

“…Systematic engineering of metabolic pathways in C. glutamicum has allowed the biochemical production of novel products, such as platform chemicals (ethanol, ethylene glycol, glycolate, 3‐hydroxypropionic acid, 1,2‐propanediol, 1,3 propanediol, 2,3‐butanediol, and isobutanol) and polyamide monomers (putrescine, cadaverine, gamma‐aminobutyrate, 5‐aminovalerate, succinate, and glutarate) . Recently, extensive research has focused on the sustainable production of polyamide monomers using C. glutamicum owing to its ability to overproduce important amino acid precursors, i.e., l ‐lysine and l ‐glutamate . In this review, we focus on recent advances in engineering C. glutamicum for the direct production of biochemicals and polyamide monomers from alternative carbon sources and CBP of biomass including lignocellulosic and starch biomass.…”

Section: Introductionmentioning

confidence: 99%

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Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Baritugo

Kim

David

et al. 2018

Biofuels Bioprod Bioref

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The fermentative production of platform chemicals in biorefineries is a sustainable alternative to current petroleum‐refining processes. Industrial microorganisms, such as Escherichia coli, Saccharomyces cerevisiae, and Corynebacterium glutamicum, have been engineered as microbial cell factories that are able to utilize biomass for the production of value‐added platform chemicals and polymers. Compared to E. coli and S. cerevisiae, C. glutamicum displays weak carbon catabolite repression and can co‐utilize mixed sugars as carbon sources, without any significant growth retardation. Pathways for the utilization of alternative carbon sources, such as d‐xylose and l‐arabinose from lignocellulosic biomass, lactose and galactose from whey, glycerol from biodiesel, and methanol from natural gas refineries, have been evaluated for chemical production. However, the application of C. glutamicum in biorefineries is limited because it does not secrete hydrolases for the efficient utilization of cellulose, xylan, and starch from lignocellulosic and starch biomass. To solve the limitation, C. glutamicum has been engineered for the consolidated bioprocessing of biomass by the heterologous expression of amylolytic and cellulolytic enzymes. Recently, C. glutamicum has been extensively engineered for polyamide monomer production owing to its ability to produce l‐lysine and l‐glutamate. This review summarizes recent advances in the development of C. glutamicum strains that can utilize renewable biomass resources for the production of industrially important chemicals. It highlights recent progress in metabolic engineering for the production of polyamide monomers. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

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Section: Production Of Polyamide Monomersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Baritugo

Kim

David

et al. 2018

Biofuels Bioprod Bioref

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“…Strategies for improving the material properties of bio-based polymers have also been extensively developed by constructing metabolically engineered microorganisms to produce novel polymer monomers and further polymer processing processes. [25][26][27][28][29][30][31][32][33][34] In vivo synthesis of PLA and its copolymer employing the PHA biosynthesis system is a promising solution for improving the stiff and brittle material properties of PLA, a bio-based polyester with a large commercial market, by incorporating co-monomers into the PLA backbone. Additionally, this system can be used as a microbial platform system to produce tailor-made polyesters containing novel monomers, which may have the desired material properties for commercial applications.…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis of 2‐Hydroxyacid‐Containing Polyhydroxyalkanoates by Employing butyryl‐CoA Transferases in Metabolically Engineered Escherichia coli

David

Joo

Yang

et al. 2017

Biotechnology Journal

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The authors previously reported the production of polyhydroxyalkanoates (PHAs) containing 2-hydroxyacid monomers by expressing evolved Pseudomonas sp. 6-19 PHA synthase and Clostridium propionicum propionyl-CoA transferase in engineered microorganisms. Here, the authors examined four butyryl-CoA transferases from Roseburia sp., Eubacterium hallii, Faecalibacterium prausnitzii, and Anaerostipes caccae as potential CoA-transferases to support synthesis of polymers having 2HA monomer. In vitro activity analyses of the four butyryl-CoA transferases suggested that each butyryl-CoA transferase has different activities towards 2-hydroxybutyrate (2HB), 3-hydroxybutyrate (3HB), and lactate (LA). When Escherichia coli XL1-Blue expressing Pseudomonas sp. 6-19 PhaC1437 along with one butyryl-CoA transferase is cultured in chemically defined MR medium containing 20 g L of glucose, 2 g L of sodium 3-hydroxybutyrate, and various concentrations of sodium 2-hydroxybutyrate, PHAs consisting of 3HB, 2HB, and LA are produced. The monomer composition of PHAs agreed well with the substrate specificities of butyryl-CoA transferases from E. hallii, F. prausnitzii, and A. caccae, but not Roseburia sp. When E. coli XL1-Blue expressing PhaC1437 and E. hallii butyryl-CoA transferase is cultured in MR medium containing 20 g L of glucose and 2 g L of sodium 2-hydroxybutyrate, P(65.7 mol% 2HB-co-34.3 mol% LA) is produced with the highest PHA content of 30 wt%. Butyryl-CoA transferases also supported the production of P(3HB-co-2HB-co-LA) from glucose as the sole carbon source in E. coli XL1-Blue strains when one of these bct genes is expressed with phaC1437, cimA3.7, leuBCD, panE, and phaAB genes. Butyryl-CoA transferases characterized in this study can be used for engineering of microorganisms that produce PHAs containing novel 2-hydroxyacid monomers.

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“…Biosynthesis of succinic acid, especially, has economically compatible productivity and is listed as a top value‐added chemical from biomass (Werpy et al, ). Glutaric acid is also an attractive compound, since the production of its precursor molecules, such as lysine and 5‐aminovaleric acid (AMV), using microorganisms, is well established (Adkins et al, ; Eggeling & Bott, ; Joo et al, ; Jorge, Pérez‐García, & Wendisch, ; Li et al, ; Park et al, ; Rohles et al, ; Wang, Cai, Chen, & Ouyang, ).…”

Section: Introductionmentioning

confidence: 99%

Enhanced production of glutaric acid by NADH oxidase and GabD‐reinforced bioconversion from l‐lysine

Hong

Moon

Choi

et al. 2018

Biotech & Bioengineering

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Glutaric acid is a promising alternative chemical to phthalate plasticizer since it can be produced by the bioconversion of lysine. Though, recent studies have enabled the high-yield production of its precursor, 5-aminovaleric acid (AMV), glutaric acid production via the AMV pathway has been limited by the need for cofactors. Introduction of NAD(P)H oxidase (Nox) withGabTD enzyme remarkably diminished the demand for oxidized nicotinamide adenine dinucleotide (NAD + ). Supply of oxygen through vigorous shaking had a significant effect on the conversion of AMV with a reduced requirement of NAD + . A high conversion rate was achieved in Nox coupled GabTD reaction under optimized expression vector, terrific broth (TB), and pH 8.5 at high cell density. Supplementary expression of GabD resulted in the production of 353 ± 35 mM glutaric acid with 88.3 ± 8.7% conversion from 400 mM AMV. Moreover, the reaction with a higher concentration of AMV could produce 528 ± 21 mM glutaric acid with 66.0 ± 2.7% conversion. In addition, the co-biotransformation strategy of GabTD and DavBA whole cells could produce 282 mM glutaric acid with 70.8% conversion from lysine, compared to the 111 mM glutaric acid yield from the combined GabTD-DavBA system.

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Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process

Cited by 50 publications

References 31 publications

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Biosynthesis of 2‐Hydroxyacid‐Containing Polyhydroxyalkanoates by Employing butyryl‐CoA Transferases in Metabolically Engineered Escherichia coli

Enhanced production of glutaric acid by NADH oxidase and GabD‐reinforced bioconversion from l‐lysine

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