Production of 6APA in the Penicillin G Fermentation Plant by Using Fiber-Entrapped Penicillin Amidase

Penicillin G acylase (PGA) is an important enzyme for the industrial production of 7-amino-3-deacetoxy cephalosporanic acid (7-ADCA) from cephalosporin G (Ceph-G), and 6-aminopenicillanic acid (6-APA) from penicillin G (Pen-G). These products are used for the manufacture of semi-synthetic cephalosporins and penicillins. In this study, immobilized PGA was utilized to catalyze the conversion of Ceph-G to 7-ADCA. The optimal conditions were found to be an operating temperature of 45• C, 0.2 M phosphate buffer, a substrate concentration of 30 mg cm −3 and a catalyst particle concentration of 0.01 g cm −3 (specific activity of 623.2 U g −1 ). Up to 45• C the reaction was characterized by an activation energy of 38.66 kJ mol −1 . Beyond 57.5• C there was a sharp decline of activity, characterized by a deactivation energy of 235.88 kJ mol −1 .

Section: Introductionmentioning

confidence: 99%

A thermal study on the use of immobilized penicillin G acylase in the formation of 7‐amino‐3‐deacetoxy cephalosporanic acid from cephalosporin G

Pan

Syu

2004

Kinetic investigation of penicillin g acylase from a mutant strain of Escherichia coli ATCC 11105 immobilized on oxirane–acrylic beads

Erarslan

Güray

1991

Thermal inactivation kinetics of penicillin g acylase obtained from a mutant derivative of escherichia coli atcc 11105

Erarslan

Koçer

1992

Thermal inactivation kinetics of native and glutaraldehyde cross‐linked forms of penicillin G acylase obtained from a mutant derivative of Escherichia coli ATCC 11105 were studied. Apparent activation energies for thermal inactivation of both native and cross‐linked forms of enzyme were calculated to be [57‐71 ± 8.46] and [67.11 ± 13.83] kcal mol−1 respectively. This slight increase in activation energy‐suggested that glutaraldehyde cross‐linking did not markedly protect against thermal inactivation. Cross‐linked enzyme did, however, have a significantly improved half‐life at temperatures between 40°C and 50°C.