Production of 7,10-dihydroxy-8(E)-octadecenoic acid using cell-free supernatant of Pseudomonas aeruginosa

Novel Biobased Non-Isocyanate Polyurethanes from Microbially Produced 7,10-Dihydroxy-8(E)-Octadecenoic Acid for Potential Packaging and Coating Applications

“…The temperatures of the detector and injector were fixed at 280 °C and 230 °C, respectively. The methyl ester of heptadecanoic acid (C17:0) was used as an internal standard for quantification …”

“…Furthermore, sodium dodecyl–sulfate polyacrylamide gel electrophoresis analysis was also performed to gain clear insights into the enzymes present in the cell-free supernatant. Three protein bands were predicted to correspond to diol synthase isoforms (120–125 and 75–80 kDa) and dioxygenase (40–50 kDa) . In this study, a maximum DOD concentration of 6.4 g/L with 74% yield was attained at 36 h of biotransformation using the cell-free batch process.…”

“…DOD was synthesized in accordance with the method described by our group previously . For the present study, preoptimized culture conditions were followed for a higher DOD yield .…”

“…Hence, the utilization of biopolymers for the plastic, chemical, and material production has directed the attention of researchers and stakeholders to innovate and develop a new sustainable, cost-effective, and greener process . Furthermore, profiling the greener process, the produced biopolymer should possess similar/superior properties, biocompatibility, and biodegradability along with the sustainable greener approach for the practical implementation and upscaling . In addition, biopolymers have higher oxygen functionality in comparison to the petro-derived polymers, further confirming their candidature to explore new high performing and advantaged polymers …”

“…Hydroxy fatty acids (HFAs) are generally formed in the plant systems and contain one or more hydroxyl group in their molecular structure with a long carbon chain with the terminal carboxylic acid group . Due to this structural peculiarity, HFAs possess higher reactivity, viscosity, solvent miscibility, and stability as compared to normal fatty acids. , These distinct chemical properties of HFA have gained industrial interest in various applications such as resins, waxes, nylons, plastics, lubricants, cosmetics, and additives in coatings and paintings. However, the production of HFA from plant resources is inadequate as per the current industrial demand which led the researchers to focus on the alternative process such as the bioconversion of different unsaturated fatty acids.…”