Production of a Bilayered Self-Assembled Skin Substitute Using a Tissue-Engineered Acellular Dermal Matrix

Cloutier, Chanel Beaudoin; Guignard, Rina; Bernard, Geneviève; Gauvin, Robert; Larouche, Danielle; Lavoie, Amélie; Lacroix, Dan; Moulin, Véronique J.; Germain, Lucie; Auger, François A.

doi:10.1089/ten.tec.2015.0258

Cited by 23 publications

(14 citation statements)

References 41 publications

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“…17–19 In all instances, inclusions disappear following skin graft integration and maturation and are not considered to be clinically significant. 17–19 …”

Section: Discussionmentioning

confidence: 99%

Improved Methods to Produce Tissue-Engineered Skin Substitutes Suitable for the Permanent Closure of Full-Thickness Skin Injuries

Larouche

Cantin-Warren

Desgagné

et al. 2016

BioResearch Open Access

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There is a clinical need for skin substitutes to replace full-thickness skin loss. Our group has developed a bilayered skin substitute produced from the patient's own fibroblasts and keratinocytes referred to as Self-Assembled Skin Substitute (SASS). After cell isolation and expansion, the current time required to produce SASS is 45 days. We aimed to optimize the manufacturing process to standardize the production of SASS and to reduce production time. The new approach consisted in seeding keratinocytes on a fibroblast-derived tissue sheet before its detachment from the culture plate. Four days following keratinocyte seeding, the resulting tissue was stacked on two fibroblast-derived tissue sheets and cultured at the air–liquid interface for 10 days. The resulting total production time was 31 days. An alternative method adapted to more contractile fibroblasts was also developed. It consisted in adding a peripheral frame before seeding fibroblasts in the culture plate. SASSs produced by both new methods shared similar histology, contractile behavior in vitro and in vivo evolution after grafting onto mice when compared with SASSs produced by the 45-day standard method. In conclusion, the new approach for the production of high-quality human skin substitutes should allow an earlier autologous grafting for the treatment of severely burned patients.

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“…17–19 In all instances, inclusions disappear following skin graft integration and maturation and are not considered to be clinically significant. 17–19 …”

Section: Discussionmentioning

confidence: 99%

Improved Methods to Produce Tissue-Engineered Skin Substitutes Suitable for the Permanent Closure of Full-Thickness Skin Injuries

Larouche

Cantin-Warren

Desgagné

et al. 2016

BioResearch Open Access

Self Cite

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“…Briefly, the dermal layer is composed of stacked fibroblast sheets and keratinocytes are seeded onto the tissue, forming a stratified and cornified epidermis. Auger and Germain’s group have optimized this protocol and studied this model in vitro and in vivo (athymic mice with full-thickness skin injuries), demonstrating timely production of CSSs that could improve clinical availability for the effective wound coverage of patients 77 – 80 .…”

Section: Human Adult Skin Cells In Tesssmentioning

confidence: 99%

Cellular human tissue-engineered skin substitutes investigated for deep and difficult to heal injuries

et al. 2021

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Wound healing is an important function of skin; however, after significant skin injury (burns) or in certain dermatological pathologies (chronic wounds), this important process can be deregulated or lost, resulting in severe complications. To avoid these, studies have focused on developing tissue-engineered skin substitutes (TESSs), which attempt to replace and regenerate the damaged skin. Autologous cultured epithelial substitutes (CESs) constituted of keratinocytes, allogeneic cultured dermal substitutes (CDSs) composed of biomaterials and fibroblasts and autologous composite skin substitutes (CSSs) comprised of biomaterials, keratinocytes and fibroblasts, have been the most studied clinical TESSs, reporting positive results for different pathological conditions. However, researchers’ purpose is to develop TESSs that resemble in a better way the human skin and its wound healing process. For this reason, they have also evaluated at preclinical level the incorporation of other human cell types such as melanocytes, Merkel and Langerhans cells, skin stem cells (SSCs), induced pluripotent stem cells (iPSCs) or mesenchymal stem cells (MSCs). Among these, MSCs have been also reported in clinical studies with hopeful results. Future perspectives in the field of human-TESSs are focused on improving in vivo animal models, incorporating immune cells, designing specific niches inside the biomaterials to increase stem cell potential and developing three-dimensional bioprinting strategies, with the final purpose of increasing patient’s health care. In this review we summarize the use of different human cell populations for preclinical and clinical TESSs under research, remarking their strengths and limitations and discuss the future perspectives, which could be useful for wound healing purposes.

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“…Moreover, these in vitro engineered tissues were able to deliver cytokines, chemokines, and growth factors at the grafted site, improving the wound closure [36,[40][41][42][43]. Because of all these characteristics, selfassembled human skin is clinically used for wound healing and burn treatments [44,45]. Selfassembled skin substitutes possess a near-to-native architecture and maintain their cell growth New and Improved Tissue Engineering Techniques: Production of Exogenous Material-Free Stroma by the Self-Assembly Technique http://dx.doi.org/10.5772/62588 potential and matrix deposition.…”

Section: Engineered Human Skin Substitutesmentioning

confidence: 99%

New and Improved Tissue Engineering Techniques: Production of Exogenous Material-Free Stroma by the Self-Assembly Technique

Saba¹,

Jakubowska²,

Chabaud³

et al. 2016

Composition and Function of the Extracellular Matrix in the Human Body

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Tissue engineering results from the use of cells and scaffolds to reproduce structural and spatial organization or function of a tissue. The Production of an ideal engineered tissue depends on its designed purpose. For clinical applications, the main concerns are biocompatibility and the generation of a tissue able to mimic most of its original biological functions. Moreover, the viability of an implanted tissue is associated with its stability to support vascular networks. This chapter summarizes the theory of the self-assembly approach for tissue engineering. Adjustments and modifications in stromal thickness and extracellular matrix composition for various self-assembled tissues are discussed. Methods developed to generate tissue closely mimicking the native morphology and structure, to incorporate capillary-like networks, and to reduce production time and costs are also reviewed. The self-assembly technique leads to the production of a stroma free of exogenous material and can be adapted to generate fastest, inexpensive, and near-tonative tissue bioengineering for medical and fundamental research applications.

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Production of a Bilayered Self-Assembled Skin Substitute Using a Tissue-Engineered Acellular Dermal Matrix

Cited by 23 publications

References 41 publications

Improved Methods to Produce Tissue-Engineered Skin Substitutes Suitable for the Permanent Closure of Full-Thickness Skin Injuries

Improved Methods to Produce Tissue-Engineered Skin Substitutes Suitable for the Permanent Closure of Full-Thickness Skin Injuries

Cellular human tissue-engineered skin substitutes investigated for deep and difficult to heal injuries

New and Improved Tissue Engineering Techniques: Production of Exogenous Material-Free Stroma by the Self-Assembly Technique

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