Production of a colchicine-induced tetraploid asparagus

Braak, J.P.; Zeilinga, A. E.

doi:10.1007/bf00042302

Cited by 12 publications

(12 citation statements)

References 6 publications

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“…Scheveningen dunes, Netherlands (Braak & Zeilinga, 1957). Kynance Cove, Cornwall (Davies & Willis, 1959; F. M. Hetherington, unpublished data).…”

Section: Asparagus Prostratusmentioning

confidence: 99%

“…Many investigators have reported producing tetraploid and other polyploid cell lines, plants or clones of A. officinalis, both by colchicine or other treatments deliberately intended to induce chromosome doubling (e.g. Braak & Zeilinga, 1957;Ellison & Tiangco, 1970;McCollum, 1988) and as a result of the use of micropropagation techniques, especially callus or anther culture (Reuther, 1983;Skiebe et al, 1991;Kohmura et al, 1995). Colchicine treatment has been found to be most effective (Skiebe et al, 1991).…”

Section: Asparag Us Officinaljsmentioning

confidence: 99%

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Taxonomy of the western European endemic Asparagus prostratus (A. officinalis subsp. prostratus) (Asparagaceae)

Kay

Davies

Rich

2001

Botanical Journal of the Linnean Society

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Asparagus prostratus Dumort., (wild asparagus) and A. officinalis L. (cultivated asparagus), often regarded as subspecies or varieties of a single species, have been confused for historical and nomenclatural reasons. A taxonomic review was carried out, and they were found to be distinct species which differ in morphology (characters retained in cultivation), cytology, distribution and ecology, and they are reproductively isolated. The tetraploid A. prostratus is unlikely to be the taxon from which the diploid A. officinalis evolved. Morphological descriptions are presented, and A. prostratus is lectotypified. A. prostratus is a western European endemic of coasts of Belgium,

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“…Scheveningen dunes, Netherlands (Braak & Zeilinga, 1957). Kynance Cove, Cornwall (Davies & Willis, 1959; F. M. Hetherington, unpublished data).…”

Section: Asparagus Prostratusmentioning

confidence: 99%

Section: Asparag Us Officinaljsmentioning

confidence: 99%

Taxonomy of the western European endemic Asparagus prostratus (A. officinalis subsp. prostratus) (Asparagaceae)

Kay

Davies

Rich

2001

Botanical Journal of the Linnean Society

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“…3). A bigger diameter of spears of polyploid plants respect to the original plants has been previously described in asparagus by different authors (Braak and Zeilinga 1957;Kunitake et al 1998;Carmona-Martin et al 2015;Regalado et al 2015b). …”

Section: Discussionmentioning

confidence: 81%

Production of “super-males” of asparagus by anther culture and its detection with SSR-ESTs

Regalado

Martín

Madrid

et al. 2015

Plant Cell Tiss Organ Cult

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Anther culture is used to develop asparagus ''super-male'' (di-haploids) in asparagus, which can be used to develop ''all-male'' varieties, by crossing them with suitable females; their progenies will be formed only by males which is advantageous for producers. This report describe a new anther culture protocol adapted to ''Morado de Huétor'', a Spanish tetraploid landrace, and studied the different factors involved in callus proliferation success from anther explants such as the microspore development stage, or the type of stress used to induce the symmetric division of the microspores, to obtain a high success rate (90 %). For plantlets regenerates from anther culture (PRACs) regeneration we develop a proliferation media supplemented with a combination of pCPA and BA able to induce callus proliferation and plantlet regeneration in the same step in a 50 % of calli, simplifying the procedure. The high percentage of heterozygous male recovery, originated from somatic cells, is an important problem in the anther culture, and to elucidate the origin of PRACs we have combined different tools: ploidy analysis, characterization with the linked sex-marker Asp1-T7 and with ESTSRRs. We can establish that 50 % of PRACs obtained in this work were regenerated from diploid microspores of ''Morado de Huétor'', regenerating diploid, di-diploid and tetra-diploid plantlets. The di-diploids males (MMmm) would generate a ratio male:female of 5:1 (83.3 %) and the tetra-diploid males (MMMMmmmm) a ratio male:female of 69:1 (98.6 %), so the tetra-diploid males could be considered ''super-males'' and be used to develop ''all-male'' varieties of ''Morado de Huétor''.

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“…O critério a ser adotado depende do material, do méto-do de tratamento empregado e das possibilidades pessoais de realização das observações (4,6,15,18) . No caso presente a seleção baseou-se no aspecto morfológico das plantas, sendo que numa parte destas fez-se também a análise do pólen e do número de cromossomos.…”

Section: -Discussão E Conclusõesunclassified

Poliploidização em berinjela (Solanum melongena L.): II - Observações em plantas resultantes de tratamentos com colquicina

Medina¹,

Cruz²,

Longo³

et al. 1972

Bragantia

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SINOPSEAs observações de diversos caracteres morfológicos em plantas de berinjela (Solatium melongena L.) provenientes de tratamentos com colqui¬ cina levaram à separação de razoável número de possíveis poliplóides.O número de cromossomos determinado em células-mães de pólen revelou a existência de plantas tetraplóides e plantas quiméricas, além das normais diplóides.Numa amostra representando os diferentes tratamentos, encontrou-se uma associação quase perfeita entre a natureza tetraplóide ou quimérica da planta e a irregularidade do tamanho do pólen; tal associação não foi encontrada quando se analisou a quantidade de pólen vazio.Os frutos tetraplóides obtidos produziram sementes maiores e em nú-mero bem menor que os diplóides de pesos equivalentes.

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Production of a colchicine-induced tetraploid asparagus

Cited by 12 publications

References 6 publications

Taxonomy of the western European endemic Asparagus prostratus (A. officinalis subsp. prostratus) (Asparagaceae)

Taxonomy of the western European endemic Asparagus prostratus (A. officinalis subsp. prostratus) (Asparagaceae)

Production of “super-males” of asparagus by anther culture and its detection with SSR-ESTs

Poliploidização em berinjela (Solanum melongena L.): II - Observações em plantas resultantes de tratamentos com colquicina

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