Production of a complete set of wheat–barley group-7 chromosome recombinants with increased grain β-glucan content

Danilova, Tatiana; Poland, Jesse; Friebe, Bernd

doi:10.1007/s00122-019-03411-3

Cited by 22 publications

(15 citation statements)

References 69 publications

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“…In closely related homoeologues, such as chromosomes of the A genome of wheat and the A m genome of Triticum monococcum , the absence of Ph1 produces homologous‐like levels of CO and the resulting genetic maps are essentially identical to those produced by CO of homologues (Dubcovsky et al ., 1995; Luo et al ., 2000). With distantly related chromosomes, such as homoeologues from wheat and barley ( Hordeum vulgare ) (Danilova et al ., 2019; Rey et al ., 2015) or wheat and rye (Lukaszewski, 2000; Lukaszewski et al ., 2004), the CO frequencies are much lower, and are further reduced by any structural differences, to the point where attempts at induced recombination are futile (Lukaszewski et al ., 2001). At the same time, even within a single genome, such as that of rye, there can be very wide variation in the frequency of homoeologous pairing of individual chromosome arms with wheat (Naranjo et al ., 1988; Naranjo and Fernandez‐Rueda, 1996) and consequent widely ranging CO rates.…”

Section: Discussionmentioning

confidence: 99%

“…In chromosome engineering exercises in wheat a mutation of Ph1 , ph1b (in fact it is a deletion of a segment of chromosome 5BL with the locus, Gill et al ., 1993), is commonly used but a substitution of chromosome 5B, either by 5D or by some other group‐5 chromosome, is as effective. These have been used to induce pairing of wheat chromosomes with their homoeologues from related species, for example, Hordeum vulgare , S. cereale , Haynaldia villosa, Agropyron elongatum, various Aegilops species, and others (Sears, 1981; Lukaszewski, 2000; Zhao et al ., 2013; Rey et al ., 2015; Lukaszewski and Cowger, 2017; Zhang et al ., 2018; Danilova et al ., 2019). The frequencies of such ph1b ‐induced homoeologous CO vary widely.…”

Section: Introductionmentioning

confidence: 99%

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Some characteristics of crossing over in induced recombination between chromosomes of wheat and rye

et al. 2021

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Allopolyploid wheat (Triticum aestivum L.) carries three pairs of homoeologous genomes but its meiotic pairing is diploid-like. This is the effect of the Ph (pairing homoeologous) system which restricts chromosome pairing to strictly homologous. Ph1 is the locus with the strongest effect. Disabling Ph1 permits pairing between homoeologues and is routinely used in chromosome engineering to introgress alien variation into breeding stocks. Whereas the efficiency of Ph1 and the general pattern of homoeologous crossovers in its absence are quite well known from numerous studies, other characteristics of such crossovers remain unknown. This study analyzed the crossover points in four sets of the ph1b-induced recombinants between wheat homologues as well as between three wheat and rye (Secale cereale) homoeologous chromosome arms, and compared them to crossovers between homologues in a reference wheat population. The results show the Ph1 locus also controls crossing over of homologues, and the general patterns of homologous (with Ph1) and homoeologous (with ph1b) crossing over are the same. In all intervals analyzed, homoeologous crossovers fell within the range of frequency distribution of homologous crossovers among individual families of the reference population. No specific DNA sequence characteristics were identified that could be recognized by the Ph1 locus; the only difference between homologous and homoeologous crossing over appears to be in frequency. It is concluded that the Ph1 locus likely recognizes DNA sequence similarity; crossing over is permitted between very similar sequences. In the absence of Ph1 dissimilarities are ignored, in proportion to the level of the sequence divergence.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Some characteristics of crossing over in induced recombination between chromosomes of wheat and rye

et al. 2021

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“…To enhance βG content in wheat, different groups tried to introgress key genes from barley through the creation of sets of interspecific addition/substitution lines carrying the chromosome with major QTLs ( Danilova et al, 2019 ; Colasuonno et al, 2020 ). This approach was unable to balance barley βG content in wheat, supporting the hypothesis that multiple QTLs cooperate in the control of βG content, thus multiple key sequences need to be identified and joined to define a complete haplotype.…”

Section: β-Glucan Genetic Variability: a Source To Boost β-Glucan Content And Viscosity And To Fine-tune Its Structurementioning

confidence: 99%

The Triple Jags of Dietary Fibers in Cereals: How Biotechnology Is Longing for High FiberGrains

Botticella¹,

Savatin²,

Sestili³

2021

Front. Plant Sci.

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Cereals represent an important source of beneficial compounds for human health, such as macro- and micronutrients, vitamins, and bioactive molecules. Generally, the consumption of whole-grain products is associated with significant health benefits, due to the elevated amount of dietary fiber (DF). However, the consumption of whole-grain foods is still modest compared to more refined products. In this sense, it is worth focusing on the increase of DF fractions inside the inner compartment of the seed, the endosperm, which represents the main part of the derived flour. The main components of the grain fiber are arabinoxylan (AX), β-glucan (βG), and resistant starch (RS). These three components are differently distributed in grains, however, all of them are represented in the endosperm. AX and βG, classified as non-starch polysaccharides (NSP), are in cell walls, whereas, RS is in the endosperm, being a starch fraction. As the chemical structure of DFs influences their digestibility, the identification of key actors involved in their metabolism can pave the way to improve their function in human health. Here, we reviewed the main achievements of plant biotechnologies in DFs manipulation in cereals, highlighting new genetic targets to be exploited, and main issues to face to increase the potential of cereals in fighting malnutrition.

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“…However, most of the genes present in grass species are conserved, and the gene order among them is mainly collinear 44,45 . The long arm of barley chromosome 7H that harbours Rph3 is syntenic with the long arm of chromosome 7 in the wheat A, B, and D genomes 46 . We showed that micro-synteny is well conserved in the vicinity of Rph3 between barley and wheat genomes (SI Appendix, Fig.…”

Section: Transgenic Complementation Of Rph3mentioning

confidence: 99%

The barley leaf rust resistance gene Rph3 encodes a putative executor protein

Dinh

Singh

Cruz

et al. 2021

Preprint

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Host resistance is considered the most effective means to control plant diseases; however, individually deployed resistance genes are often rapidly overcome by pathogen adaptation. Combining multiple effective resistance genes is the optimal approach to durable resistance, but the lack of functional markers for resistance genes has hampered implementation. Leaf rust, caused by Puccinia hordei, is an economically significant disease of barley, but only a few major Resistance genes to P. hordei (Rph) have been cloned. In this study, gene Rph3 was isolated by positional cloning and confirmed by mutational analysis and transgenic complementation. The Rph3 gene, which originated from wild barley and was first introgressed into cultivated Egyptian germplasm, encodes a unique transmembrane resistance protein that differs from all known plant disease resistance proteins at the amino acid sequence level. Genetic profiles of diverse accessions indicated limited genetic diversity in Rph3 in domesticated germplasm, and higher diversity in wild barley from the Eastern Mediterranean region. Expression profiling using P. hordei isolates with contrasting pathogenicity for the Rph3 host locus showed that the Rph3 gene was expressed only in interactions with Rph3-avirulent isolates, a phenomenon also observed for transcription activator-like effector-dependent genes known as executors conferring resistance to Xanthomonas spp. Like the known transmembrane executors such as Bs3 and Xa7 heterologous expression of Rph3 in N. benthamiana induced a cell death response. Given that Rph3 shares several features with executor genes, it seems likely that P. hordei contains effectors similar to the transcription activator-like effectors that target host executor genes. The isolation of Rph3 highlights convergent evolutionary processes in diverse plant-pathogen interaction systems, where similar defence mechanisms evolved independently in monocots and dicots and provide evidence for executor genes in the Triticeae tribe.

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Production of a complete set of wheat–barley group-7 chromosome recombinants with increased grain β-glucan content

Cited by 22 publications

References 69 publications

Some characteristics of crossing over in induced recombination between chromosomes of wheat and rye

Some characteristics of crossing over in induced recombination between chromosomes of wheat and rye

The Triple Jags of Dietary Fibers in Cereals: How Biotechnology Is Longing for High FiberGrains

The barley leaf rust resistance gene Rph3 encodes a putative executor protein

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