Production of a desirable Brassica oleracea<scp>CMS</scp> line using an alloplasmic B. rapa<scp>CMS</scp> line carrying Diplotaxis erucoides cytoplasm as a bridge plant

Fujita, Yoshiaki; Sunaga, Keita; Shim, Suhyeun; Yamada, Wataru; Ohnishi, Takayuki; Bang, Sang Woo

doi:10.1111/pbr.12565

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…When two species are cross-incompatible in nature but bridge species has cross compatibility with both the species. Fujita et al (2017) [15] transfer of Diplotaxis erucoides cytoplasm to B. oleracea lines where B. rapa used as a bridge species to avoid incompatibility between donor and recipient parent. D. erucoides is not directly crossable with B. oleracea.…”

Section: Use Of Bridge Speciesmentioning

confidence: 99%

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Distant hybridization and introgression of alien genes for improving heterotic potential and resistance to various biotic stresses in vegetable and oilseed brassicas (Brassica Sp.)

Das¹

2020

Int. J. Chem. Stud.

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Family Brassicaceae comprised of diverse crop species which are highly nutritional, economically high valued and used as oilseed as well as vegetable in our daily diet. However, yield and productivity of these crops become reduced due to several biotic stresses as well as susceptibility to weeds. Additionally, to meet the growing demand of famers, F1 hybrid development is the foremost priority to breeders with the help of any economically feasible genetically controlled system. Thus, researchers are continuously searching for stable male sterility systems for quality hybrid development and for resistant genes against various biotic stresses and concurrently, introgression of desirable gene into cultivated species though distant hybridization method by crossing with its distantly related species (Inter-specific) or genus (Intergeneric). However, to overcome pre and post-zygotic crossing barriers, various in-vitro techniques like embryo rescue, protoplast fusion, chromosome doubling or growth regulators can be used to convert sterile hybrid into fertile one.

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Section: Use Of Bridge Speciesmentioning

confidence: 99%

“…After consecutive three years of backcrossing BC3 plants produced white colour curd similar to cauliflower with male sterile flower. [12] Brassica rapa as bridge species (Diplotaxis erucoides cytoplasm) Brassica oleracea -Fujita et al, 2017 [15] Transfer of S-alleles for…”

Section: Transfer Of Male Sterilitymentioning

confidence: 99%

Distant hybridization and introgression of alien genes for improving heterotic potential and resistance to various biotic stresses in vegetable and oilseed brassicas (Brassica Sp.)

Das¹

2020

Int. J. Chem. Stud.

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Properties of self-sterile but cross-fertile allopolyploids synthesized between Brassica rapa and Raphanus sativus

Lee¹,

Son²,

Kim³

et al. 2020

Hortic. Environ. Biotechnol.

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Optimizing Brassica oleracea L. Breeding Through Somatic Hybridization Using Cytoplasmic Male Sterility (CMS) Lines: From Protoplast Isolation to Plantlet Regeneration

Romero-Muñoz,

Pérez-Jiménez

2024

Plants

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The Brassica oleracea L. species embrace important horticultural crops, such as broccoli, cauliflower, and cabbage, which are highly valued for their beneficial nutritional effects. However, the complexity of flower emasculation in these species has forced breeders to adopt biotechnological approaches such as somatic hybridization to ease hybrid seed production. Protoplasts entail a versatile tool in plant biotechnology, supporting breeding strategies that involve genome editing and hybridization. This review discusses the use of somatic hybridization in B. oleracea L. as a biotechnological method for developing fusion products with desirable agronomic traits, particularly cytoplasmic male sterile (CMS) condition. These CMS lines are critical for implementing a cost-effective, efficient, and reliable system for producing F1 hybrids. We present recent studies on CMS systems in B. oleracea L. crops, providing an overview of established models that explain the mechanisms of CMS and fertility restoration. Additionally, we emphasize key insights gained from protoplast fusion applied to B. oleracea L. breeding. Key steps including pre-treatments of donor plants, the main tissues used as sources of parental protoplasts, methods for obtaining somatic hybrids and cybrids, and the importance of establishing a reliable plant regeneration method are discussed. Finally, the review explores the incorporation of genome editing technologies, such as CRISPR-Cas9, to introduce multiple agronomic traits in Brassica species. This combination of advanced biotechnological tools holds significant promise for enhancing B. oleracea breeding programs in the actual climate change context.

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Production of a desirable Brassica oleraceaCMS line using an alloplasmic B. rapaCMS line carrying Diplotaxis erucoides cytoplasm as a bridge plant

Cited by 3 publications

References 32 publications

Distant hybridization and introgression of alien genes for improving heterotic potential and resistance to various biotic stresses in vegetable and oilseed brassicas (Brassica Sp.)

Distant hybridization and introgression of alien genes for improving heterotic potential and resistance to various biotic stresses in vegetable and oilseed brassicas (Brassica Sp.)

Properties of self-sterile but cross-fertile allopolyploids synthesized between Brassica rapa and Raphanus sativus

Optimizing Brassica oleracea L. Breeding Through Somatic Hybridization Using Cytoplasmic Male Sterility (CMS) Lines: From Protoplast Isolation to Plantlet Regeneration

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