1989
DOI: 10.1016/0378-1119(89)90421-6
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Production of a hybrid macrolide antibiotic in Streptomyces ambofaciens and Streptomyces lividans by introduction of a cloned carbomycin biosynthetic gene from Streptomyces thermotolerans

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Cited by 90 publications
(28 citation statements)
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“…The asm cluster contains candidate genes for all of the steps required to elaborate the final ansamitocin structure from proansamitocin. asm19 is related to mdmB from Streptomyces mycarofaciens and acyA from Streptomyces thermotolerans, which encode macrolide O-acyltransferases (30,47). The functional assignment as an acyl- transferase attaching the C-3 ester group of ansamitocin was verified by inactivating asm19 through an internal 549-bp in frame deletion.…”
Section: Identification and Cloning Of The Ansamitocin Biosynthetic Genementioning
confidence: 99%
See 1 more Smart Citation
“…The asm cluster contains candidate genes for all of the steps required to elaborate the final ansamitocin structure from proansamitocin. asm19 is related to mdmB from Streptomyces mycarofaciens and acyA from Streptomyces thermotolerans, which encode macrolide O-acyltransferases (30,47). The functional assignment as an acyl- transferase attaching the C-3 ester group of ansamitocin was verified by inactivating asm19 through an internal 549-bp in frame deletion.…”
Section: Identification and Cloning Of The Ansamitocin Biosynthetic Genementioning
confidence: 99%
“…Three AdoMetdependent methyltransferase genes, asm7, asm10, and asm17, were identified in the asm cluster. asm17, which is homologous to several O-methyltransferase genes (30,47,53), because of its linkage to asm13-16, is assigned to the O-methylation at C-10. Asm7 is related to Streptomyces antibiotic biosynthetic Omethyltransferases (35,54,55) and may be responsible for either the N-methylation or the C-20 O-methylation.…”
Section: Identification and Cloning Of The Ansamitocin Biosynthetic Genementioning
confidence: 99%
“…These proteins include O-acetyl transferase from Shigella flexneri bacteriophage SF6, which acetylates the bacterial O-antigen conferring new antigenic groups (4,30); NodX from Rhizobium leguminosarum biovar viciae, which is responsible for acetylation of Nod factor and confers host range specificity (5,8); ExoZ from Rhizobium meliloti, which is responsible for acetylation of exopolysaccharide (2,22); and several proteins from Streptomyces spp. that acylate macrolide antibiotics (1,7,11). Acetylation of many of these substrates has a profound effect on the interaction of the bacterium with the corresponding host organism.…”
Section: Vol 178 1996mentioning
confidence: 99%
“…So far, only a few O-methylating enzymes have been shown, which modify sugar moieties, including the already mentioned Omethyltransferases involved in macrolide biosyntheses. In addition, other sugar-modifying enzymes (acyltransferases) have been reported from macrolide producers (33)(34)(35). As far as we know, no methyltransferases or acyltransferases involved in modifying the glycosyl moieties of aromatic polyketides have been characterized before.…”
Section: Discussionmentioning
confidence: 99%