Production of a lignocellulolytic enzyme system for simultaneous bio-delignification and saccharification of corn stover employing co-culture of fungi

Ma, Kedong; Ruan, Zhiyong

doi:10.1016/j.biortech.2014.10.161

Cited by 88 publications

(32 citation statements)

References 34 publications

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“…This result is in agreement with Ma and Ruan (2015), who detected low activity levels for hydrolytic enzymes such as CMCase and xylanase during mycelial growth of the white-rot fungus Coprinus comatus on corn stover. However, xylanase activity was found simultaneously with ligninolytic enzymes by the seven strains during the biodegradation of SCB, showing higher activity than laccase and LiP.…”

Section: Resultssupporting

confidence: 93%

“…In a second step, the reactive radical can be cleaved, modified by changes in its functional group or be attached to other Trametes hirsuta yj9 and laccase played a main role in delignification. In line with these results, Ma and Ruan (2015) reported that laccase was the main enzyme synthesized by…”

Section: Biological Pretreatmentsupporting

confidence: 65%

“…Sun et al (2011) detected cellulase (up to 2.86 U/g solid) and large amounts of xylanase (up to 11.22 U/g solid) during the 42-days incubation of corn stover with T. hirsuta yj9. On the other hand, according to Ma and Ruan (2015) very little hydrolytic enzymes were secreted during corn stover biodelignification, as long as cellulase and xylanase production exhibited considerable low activity. Aligning, El-Nasser; Helmy;…”

Section: Biological Pretreatmentmentioning

confidence: 99%

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Evaluation of microorganisms performance in hemicellulosic hydrolyzate detoxification and sugarcane bagasse lignin removal for ethanol production

Gonçalves¹

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Section: Resultssupporting

confidence: 93%

Section: Biological Pretreatmentsupporting

confidence: 65%

Section: Biological Pretreatmentmentioning

confidence: 99%

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Evaluation of microorganisms performance in hemicellulosic hydrolyzate detoxification and sugarcane bagasse lignin removal for ethanol production

Gonçalves¹

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“…There is substantial literature on the effects at the enzymatic level of mixed cultures with T. reesei, which is one of the most studied fungal species in mixed cultures [159,[161][162][163][164]. Two of these studies combined ascomycetes and basidiomycetes [161,163] with one of the studies performing saccharification assays using enzyme cocktails from the mono and mixed cultures [163].…”

Section: Mixed Cultures At the Enzymatic Levelmentioning

confidence: 99%

“…Two of these studies combined ascomycetes and basidiomycetes [161,163] with one of the studies performing saccharification assays using enzyme cocktails from the mono and mixed cultures [163]. In the study of Ma and Ruan [163], there was a clear synergistic effect on saccharification using culture supernatant from a mixed culture of an ascomycete T. reesei and a basidiomycete Coprinus comatus. Here the de-lignifying enzymes of the basidiomycete resulted in the saccharification of a greater proportion of the polysaccharides by T. reesei CAZymes.…”

Section: Mixed Cultures At the Enzymatic Levelmentioning

confidence: 99%

Transcriptional Regulation and Responses in Filamentous Fungi Exposed to Lignocellulose

2015

Mycology: Current and Future Developments

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Biofuels derived from lignocellulose are attractive alternative fuels but their production suffers from a costly and inefficient saccharification step that uses fungal enzymes. One route to improve this efficiency is to understand better the transcriptional regulation and responses of filamentous fungi to lignocellulose. Sensing and initial contact of the fungus with lignocellulose is an important aspect. Differences and similarities in the responses of fungi to different lignocellulosic substrates can partly be explained with existing understanding of several key regulators and their mode of action, as will be demonstrated for Trichoderma reesei, Neurospora crassa and Aspergillus spp. The regulation of genes encoding Carbohydrate Active enZymes (CAZymes) is influenced by the presence of carbohydrate monomers and short oligosaccharides, as well as the external stimuli of pH and light. We explore several important aspects of the response to lignocellulose that are not related to genes encoding CAZymes, namely the regulation of transporters, accessory proteins and stress responses. The regulation of gene expression is examined from the perspective of mixed cultures and models are presented for the nature of the transcriptional basis for any beneficial effects of such mixed cultures. Various applications in biofuel technology are based on manipulating transcriptional regulation and learning from fungal responses to lignocelluloses. Here we critically access the application of fungal transcriptional responses to industrial saccharification reactions. As part of this chapter, selected regulatory mechanisms are also explored in more detail.

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Lignin peroxidase functionalities and prospective applications

et al. 2016

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Ligninolytic extracellular enzymes, including lignin peroxidase, are topical owing to their high redox potential and prospective industrial applications. The prospective applications of lignin peroxidase span through sectors such as biorefinery, textile, energy, bioremediation, cosmetology, and dermatology industries. The litany of potentials attributed to lignin peroxidase is occasioned by its versatility in the degradation of xenobiotics and compounds with both phenolic and non‐phenolic constituents. Over the years, ligninolytic enzymes have been studied however; research on lignin peroxidase seems to have been lagging when compared to other ligninolytic enzymes which are extracellular in nature including laccase and manganese peroxidase. This assertion becomes more pronounced when the application of lignin peroxidase is put into perspective. Consequently, a succinct documentation of the contemporary functionalities of lignin peroxidase and, some prospective applications of futuristic relevance has been advanced in this review. Some articulated applications include delignification of feedstock for ethanol production, textile effluent treatment and dye decolourization, coal depolymerization, treatment of hyperpigmentation, and skin‐lightening through melanin oxidation. Prospective application of lignin peroxidase in skin‐lightening functions through novel mechanisms, hence, it holds high value for the cosmetics sector where it may serve as suitable alternative to hydroquinone; a potent skin‐lightening agent whose safety has generated lots of controversy and concern.

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Production of a lignocellulolytic enzyme system for simultaneous bio-delignification and saccharification of corn stover employing co-culture of fungi

Cited by 88 publications

References 34 publications

Evaluation of microorganisms performance in hemicellulosic hydrolyzate detoxification and sugarcane bagasse lignin removal for ethanol production

Evaluation of microorganisms performance in hemicellulosic hydrolyzate detoxification and sugarcane bagasse lignin removal for ethanol production

Transcriptional Regulation and Responses in Filamentous Fungi Exposed to Lignocellulose

Lignin peroxidase functionalities and prospective applications

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