Production of a monoclonal antibody against cell-surface glycoprotein of guinea pig adrenocortical cells.

Abstract

A monoclonal antibody (MAb) that reacted with the cellsurface antigens of adrenocortical cells was generated against cell suspensions from guinea pig adrenal glands. Cell-surfam membranes of the adrenocortical cells in all zones, i.e., zona glomerulosa, ZOM fasciculata, and ZOM reticularis, were labeled with the antibody. Adrenal medulla remained unlabeled. Immunoelectron microscopy showed that entire plasma membranes, i.e., plasma membranes between adjacent cells and free cell-surface membranes, including sinusoidal microvilli, were immunoreactive to the antibody. Immunoblot analysis demonstrated that the antibody bound to two prominent bands at molecular weights of approximately 62,000 and 110,000. Two bands were stained with lectin-digoxigenin conjugates. The 110 KD band reacted with Datura svamoniUm @SA) and Maackia amurensis (MAA) agglutinins, indicating the presence of N-acetyl-