Production of a monoclonal antibody against oxytetracycline and its application for oxytetracycline residue detection in shrimp

Wongtangprasert, Tossapon; Natakuathung, Wirongrong; Pimpitak, Umaporn; Buakeaw, Anumart; Palaga, Tanapat; Komolpis, Kittinan; Khongchareonporn, Nanthika

doi:10.1631/jzus.b1300181

Cited by 15 publications

(7 citation statements)

References 26 publications

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“…Based on the difference in molecular weight of 306.8 Da) and BSA (66,222.9 Da), the conjugate molar ratio of CTC:BSA was calculated to be 4:1, indicating the occurrence of coupling molecules (Figure 1(a)). This conjugation occurred at a lower ratio than our report for oxytetracycline conjugated with BSA (7:1) (Wongtangprasert et al, 2014). The Mannich reaction is a simple and one-step reaction in which the hepten density of the conjugation is dependent on the reactivity of active hydrogen within the hepten molecule.…”

Section: Chlortetracycline Conjugationcontrasting

confidence: 49%

A specific monoclonal antibody for chlortetracycline detection in milk and honey samples based on ELISA

Poungmalai

Buakeaw

Puthong

et al. 2021

Food and Agricultural Immunology

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In this study, a monoclonal antibody (mAb) against chlortetracycline was prepared by immunisation using chlortetracycline conjugated to bovine serum albumin (CTC-BSA) and cell fusion. One monoclonal antibody (1-2B) against chlortetracycline was obtained with an IC 50 of 0.33 ng/ml and a detection limit of 0.1 ng/ml. The cross reactivity of this antibody with structurally related and nonrelated compounds was less than 0.1%, demonstrating that the obtained mAb provided high specificity. Under optimal conditions for ic-ELISA, a linearity was obtained between 0.06 and 3.9 ng/ml of chlortetracycline. The quantification of CTC-spiked milk and honey samples was also performed, demonstrating recovery rates ranging from 95.2% to 105.6% and the coefficient of variation below 10% for both intra-and inter-variation assays. Based on the sensitivity and specificity measurement, mAb 1-2B could feasibility be used as a screening tool for CTC residue detection in milk and honey samples.

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Section: Chlortetracycline Conjugationcontrasting

confidence: 49%

A specific monoclonal antibody for chlortetracycline detection in milk and honey samples based on ELISA

Poungmalai

Buakeaw

Puthong

et al. 2021

Food and Agricultural Immunology

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“…ten times) in the immunoassays than in the MS analysis. Finally, recently, Wongtanprasert et al [ 103 ] developed a new monoclonal antibody against oxytetracycline and applied it for OTC quantification in shrimp using an indirect competitive ELISA with recoveries of 82 to 118%. The antibody showed high cross-reactivity to rolitetracycline but no cross-reactivity to other unrelated antibiotics.…”

Section: Analytical Methods For Tetracyclines Determination and Rementioning

confidence: 99%

Tetracyclines in Food and Feedingstuffs: From Regulation to Analytical Methods, Bacterial Resistance, and Environmental and Health Implications

Granados-Chinchilla

Rodríguez

2017

Journal of Analytical Methods in Chemistry

252

128

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Antibiotics are widely used as growth promoters in animal husbandry; among them, the tetracyclines are a chemical group of relevance, due to their wide use in agriculture, surpassing in quantities applied almost every other antibiotic family. Seeing the considerable amounts of tetracyclines used worldwide, monitoring of these antibiotics is paramount. Advances must be made in the analysis of antibiotics to assess correct usage and dosage of tetracyclines in food and feedstuffs and possible residues in pertinent environmental samples. The tetracyclines are still considered a clinically relevant group of antibiotics, though dissemination of tolerance and resistance determinants have limited their use. This review focuses on four different aspects: (i) tetracyclines, usage, dosages, and regulatory issues that govern their food-related application, with particular attention to the prohibitions and restrictions that several countries have enforced in recent years by agencies from both the United States and the European Union, (ii) analytical methods for tetracyclines, determination, and residues thereof in feedstuffs and related matrices with an emphasis on the most relevant and novel techniques, including both screening and confirmatory methods, (iii) tetracycline resistance and tetracycline-resistant bacteria in feedstuff, and (iv) environmental and health risks accompanying the use of tetracyclines in animal nutrition. In the last two cases, we discuss the more relevant undesirable effects that tetracyclines exert over bacterial communities and nontarget species including unwanted effects in farmers.

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“…protein antigens), the chance to get the effective mAbs with free hapten-binding ability is relatively low. For instance, only three hybridomas of interest were found among a total of 8700 cultured wells from ten fusions in a previous report on the production of mAbs against oxytetracycline, an antibiotic with the molecular weight of 460.4 Da (Wongtangprasert et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a water-soluble adjuvant for the development of monoclonal antibodies against small-molecule compounds

Liu

Lan

et al. 2016

J. Zhejiang Univ. Sci. B

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A water-soluble adjuvant named QuickAntibody (QA) was introduced into the procedure of mouse immunization for the development of hapten-specific monoclonal antibodies (mAbs), using four kinds of pesticides as model compounds. Compared with conventional Freund's adjuvants, QA treatments offered relatively low but acceptable antiserum titers after three inoculations, gave little adverse effects to the experimental animals, and were preferable in harvesting splenocytes during the steps of cell fusion. Afterwards, hybridomas from the QA group were prepared and screened by both non-competitive and competitive indirect enzyme-linked immunosorbent assays (ELISAs). The efficiency of gaining immune-positive hybridomas was satisfactory, and the resultant mAbs showed sensitivities (half maximal inhibitory concentration (IC 50)) of 0.91, 2.46, 3.72, and 6.22 ng/ml to triazophos, parathion, chlorpyrifos, and fenpropathrin, respectively. Additionally, the performance of QA adjuvant was further confirmed by acquiring a high-affinity mAb against okadaic acid (IC 50 of 0.36 ng/ml) after three immunizations. These newly developed mAbs showed similar or even better sensitivities compared with previously reported mAbs specific to the corresponding analytes. This study suggested that the easy-to-use adjuvant could be applicable to the efficient generation of highly sensitive mAbs against small compounds.

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Production of a monoclonal antibody against oxytetracycline and its application for oxytetracycline residue detection in shrimp

Cited by 15 publications

References 26 publications

A specific monoclonal antibody for chlortetracycline detection in milk and honey samples based on ELISA

A specific monoclonal antibody for chlortetracycline detection in milk and honey samples based on ELISA

Tetracyclines in Food and Feedingstuffs: From Regulation to Analytical Methods, Bacterial Resistance, and Environmental and Health Implications

Evaluation of a water-soluble adjuvant for the development of monoclonal antibodies against small-molecule compounds

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