Production of a Monoclonal Antibody against an Epitope on HeLa Cells that Is the Functional Poliovirus Binding Site

Nobis, Peter; Zibirre, Reinhard; Meyer, G; Kühne, J; Warnecke, G; Koch, Gebhard

doi:10.1099/0022-1317-66-12-2563

Cited by 85 publications

(64 citation statements)

References 23 publications

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“…The role of DAF (CD55), vitronectin receptor (a v b 3 ) and PVR (CD155) in cell attachment and virus internalization was studied using CPE protection assays (Nobis et al, 1985). None of the blocking antibodies was able to prevent infection in RD cells.…”

Section: Receptor Usagementioning

confidence: 99%

Enterovirus 94, a proposed new serotype in human enterovirus species D

Smura

Junttila

Blomqvist

et al. 2007

Journal of General Virology

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The genus Enterovirus (family Picornaviridae) contains five species with strains isolated from humans: Human enterovirus A (HEV-A), HEV-B, HEV-C, HEV-D and Poliovirus. In this study, a proposed new serotype of HEV-D was characterized. Four virus strains were isolated from sewage in Egypt and one strain from acute flaccid paralysis cases in the Democratic Republic of the Congo. The complete genome of one environmental isolate, the complete coding sequence of one clinical isolate and complete VP1 regions from the other isolates were sequenced. These isolates had 66.6-69.4 % nucleotide similarity and 74.7-76.6 % amino acid sequence similarity in the VP1 region with the closest enterovirus serotype, enterovirus 70 (EV70), suggesting that the isolates form a new enterovirus type, tentatively designated enterovirus 94 (EV94). Phylogenetic analyses including sequences of the 59 UTR, VP1 and 3D regions demonstrated that EV94 isolates formed a monophyletic group within the species HEV-D. No evidence of recombination was found between EV94 and the other HEV-D serotypes, EV68 and EV70. Further biological characterization showed that EV94 was acid stable and had a wide cell tropism in vitro. Attempts to prevent replication with protective antibodies to known enterovirus receptors (poliovirus receptor, vitronectin a v b 3 receptor and decay accelerating factor) were not successful. Seroprevalence studies in the Finnish population revealed a high prevalence of this virus over the past two decades. INTRODUCTIONThe genus Enterovirus (family Picornaviridae) contains a large group of human pathogens. Although the majority of enterovirus infections are subclinical, enterovirus infection can lead to a variety of acute and chronic diseases including mild upper respiratory illness, febrile rash, aseptic meningitis, encephalitis, acute haemorrhagic conjunctivitis, pleurodynia, acute flaccid paralysis (AFP), diabetes, myocarditis and neonatal sepsis-like disease (Pallansch & Roos, 2001). The primary site of enterovirus infection is the mucosal tissue of the respiratory or gastrointestinal tract. After spreading through the lymphatic system and circulation, the virus can infect secondary target tissues. The secondary replication sites largely define the clinical manifestations of a given enterovirus strain. In the intestinal mucosa, virus replication can continue for several weeks, during which time progeny virus is shed into faeces.The enterovirus genome is a single-stranded RNA molecule of approximately 7500 nt consisting of a single open reading frame flanked by non-coding 59 and 39 regions. The 59 UTR contains an internal ribosome-binding site, which is essential for translation initiation (Pelletier & Sonenberg, 1988;Molla et al., 1992;Chen & Sarnow, 1995). The 39 UTR forms highly conserved secondary and tertiary structures that are thought to be important in replication initiation (Pilipenko et al., 1992(Pilipenko et al., , 1996Mirmomeni et al., 1997). The open reading frame is translated into a single, large polypeptide, which is...

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Section: Receptor Usagementioning

confidence: 99%

Enterovirus 94, a proposed new serotype in human enterovirus species D

Smura

Junttila

Blomqvist

et al. 2007

Journal of General Virology

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“…Two of the groups isolated mAbs which blocked infection with all three serotypes ofpolio and no other enteroviruses as expected from previous kinetic binding studies (52,53). These mAbs recognized receptors only on poliovirussusceptible primate cell lines.…”

Section: Immunologic Approachesmentioning

confidence: 69%

Strategies for the identification of icosahedral virus receptors.

Bass

Greenberg²

1992

J. Clin. Invest.

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The first step in virus infection of a host cell is attachment to the cell plasma membrane. This necessary but not always sufficient event in cell infection is important for several reasons. Specific receptor expression can be an important determinant of host range and cell and tissue tropism of a virus, although productive infection may be regulated at many other stages in viral replication as well. As an initial step in infection, virus binding offers an opportunity for chemical or immunologic intervention before the viral genome has reached relative sanctuary within the cell. Finally, characterization ofviral receptors can lead to the identification of new cell surface molecules whose normal function can then be determined.A variety of strategies has been employed to identify and characterize specific viral receptors. Most of these studies, however, have not definitively demonstrated that the putative receptor actually mediates infection ofthe cell. To fully establish that an interaction between a virus and a presumed receptor has biologic significance, it is necessary to demonstrate that such an interaction can initiate viral infection of the cell. The most stringent test of biologic significance is the transformation of receptor-negative nonpermissive cells to a permissive phenotype by molecularly engineered transfer ofgenetic material encoding a putative receptor. Recently these stringent criteria have been met for a small icosahedral virus, poliovirus (1) as well as for the enveloped viruses HIV (2) and Maloney murine leukemia virus (3).This review will concentrate on recent progress in the study of cell receptors for nonenveloped, icosahedral viruses (see Table I). Icosahedral viruses present unique questions concerning the early events ofviral infection, especially those ofviral entry. The review will not be exhaustive but will use selected examples to emphasize the methodologies and experimental strategies which have made this progress possible. Several more comprehensive recent reviews of viral receptors are available (4, 5).Although

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“…All experiments were performed at least twice and qualitatively similar data were obtained. 39 the first anti-PVR antibody, was submitted to the 1993 Human Leukocyte Typing V White Cell Differentiation Antigen workshop. In this workshop, laboratories throughout the world screened numerous cell types for expression of D171 reactivity.…”

Section: Stainingmentioning

confidence: 99%

CD34+ hematopoietic stem cells support entry and replication of poliovirus: a potential new gene introduction route

et al. 2013

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Pluripotent hematopoietic stem cells (HSC) are critical in sustaining and constantly renewing the blood and immune system. The ability to alter biological characteristics of HSC by introducing and expressing genes would have enormous therapeutic possibilities. Previous unpublished work suggested that human HSC co-express CD34 (cluster of differentiation 34; an HSC marker) and CD155 (poliovirus receptor; also called Necl-5/Tage4/PVR/CD155). In the present study, we demonstrate the co-expression of CD34 and CD155 in primary human HSC. In addition, we demonstrate that poliovirus infects and replicates in human hematopoietic progenitor cell lines. Finally, we show that poliovirus replicates in CD34 þ enriched primary HSC. CD34 þ enriched HSC co-express CD155 and support poliovirus replication. These data may help further understanding of poliovirus spread in vivo and also demonstrate that human HSC may be amenable for gene therapy via poliovirus-capsid-based vectors. They may also help elucidate the normal function of Necl-5/Tage4/PVR/CD155.

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Production of a Monoclonal Antibody against an Epitope on HeLa Cells that Is the Functional Poliovirus Binding Site

Cited by 85 publications

References 23 publications

Enterovirus 94, a proposed new serotype in human enterovirus species D

Enterovirus 94, a proposed new serotype in human enterovirus species D

Strategies for the identification of icosahedral virus receptors.

CD34+ hematopoietic stem cells support entry and replication of poliovirus: a potential new gene introduction route

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