Production of a murine mAb against Bothrops alternatus and B. neuwiedi snake venoms and its use to isolate a thrombin-like serine protease fraction

Belo, Andreza Alves; Souza, Dayane Lorena Naves de; Melo‐Braga, Marcella Nunes; Souza, Letícia Lopes de; Molina, Denis Alexis Molina; Melo, Patrícia Donado Vaz de; Larsen, Martin R.; Guerra-Duarte, Clara; Chávez-Olortégui, Carlos

doi:10.1016/j.ijbiomac.2022.06.127

Cited by 3 publications

(6 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, B. jararacussu venom is highly myotoxic due to the activity of two molecules with a PLA 2 structure: the enzymatically inactive bothropstoxin I (Bthtx-I) and the enzymatically active bothropstoxin II (Bthtx-II) [ 39 , 40 ]. B. neuwiedi venom has high coagulant, hemorrhagic, and myotoxic activities [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

“…Antivenoms do not neutralize such toxic activities caused by Bothropic venoms [ 41 ] or other vipers [ 42 ] and may even induce hypersensitive reactions in patients [ 14 ]. Thus, molecules able to neutralize the toxic activities of snake venoms are required to improve the recovery of victims of SBE or to prevent sequelae.…”

Section: Discussionmentioning

confidence: 99%

“…SVSPs affect the coagulation cascade due to the action of thrombin-like enzymes (TLEs) that have fibrinogenolytic activity, leading to fibrin formation through the cleavage of fibrinogen [ 34 , 48 ]. However, other approaches to inhibit SVSPs have been investigated, such as murine monoclonal antibodies (mAbs) and synthetic peptides based on the sequence of their substrates or natural inhibitors [ 41 ]. However, mAbs failed to neutralize the toxins of B. alternatus and B. neuwiedi venoms [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, other approaches to inhibit SVSPs have been investigated, such as murine monoclonal antibodies (mAbs) and synthetic peptides based on the sequence of their substrates or natural inhibitors [ 41 ]. However, mAbs failed to neutralize the toxins of B. alternatus and B. neuwiedi venoms [ 41 ]. Da Silva et al [ 49 ] designed two synthetic peptides that inhibited SVSPs of B. jararaca crude venom and the purified serine protease Batroxobin, but not human trypsin, which is a serine protease.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Effect of Seaweed-Derived Fucoidans from Undaria pinnatifida and Fucus vesiculosus on Coagulant, Proteolytic, and Phospholipase A2 Activities of Snake Bothrops jararaca, B. jararacussu, and B. neuwiedi Venom

Castro-Pinheiro,

Junior,

Sanchez

et al. 2024

Toxins

View full text Add to dashboard Cite

Background: Snakebite envenomation (SBE) causes diverse toxic effects in humans, including disability and death. Current antivenom therapies effectively prevent death but fail to block local tissue damage, leading to an increase in the severity of envenomation; thus, seeking alternative treatments is crucial. Methods: This study analyzed the potential of two fucoidan sulfated polysaccharides extracted from brown seaweeds Fucus vesiculosus (FVF) and Undaria pinnatifida (UPF) against the fibrinogen or plasma coagulation, proteolytic, and phospholipase A2 (PLA2) activities of Bothrops jararaca, B. jararacussu, and B. neuwiedi venom. The toxicity of FVF and UPF was assessed by the hemocompatibility test. Results: FVF and UPF did not lyse human red blood cells. FVF and UPF inhibited the proteolytic activity of Bothrops jararaca, B. jararacussu, and B. neuwiedi venom by approximately 25%, 50%, and 75%, respectively, while all venoms led to a 20% inhibition of PLA2 activity. UPF and FVF delayed plasma coagulation caused by the venoms of B. jararaca and B. neuwiedi but did not affect the activity of B. jararacussu venom. FVF and UPF blocked the coagulation of fibrinogen induced by all these Bothropic venoms. Conclusion: FVF and UPF may be of importance as adjuvants for SBE caused by species of Bothrops, which are the most medically relevant snakebite incidents in South America, especially Brazil.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Seaweed-Derived Fucoidans from Undaria pinnatifida and Fucus vesiculosus on Coagulant, Proteolytic, and Phospholipase A2 Activities of Snake Bothrops jararaca, B. jararacussu, and B. neuwiedi Venom

Castro-Pinheiro,

Junior,

Sanchez

et al. 2024

Toxins

View full text Add to dashboard Cite

show abstract

“…Based on the catalytic amino acid residues they contain, four different types of SVSPs have been identified: metalloproteinases ( Modahl, Frietze, and Mackessy, 2018 ; Leonardi et al, 2019 ; Williams et al, 2019 ; Asega et al, 2020 ), serine ( Carone et al, 2018 ; Belo et al, 2022 ; Morjen et al, 2022 ), aspartic ( Teixeira et al, 2020 ), and cysteine ( Suntravat et al, 2019 ; Tadokoro et al, 2020 ; Suntravat et al, 2021 ; Požek et al, 2022 ). Serine proteases are the most prevalent and extensively researched group of these.…”

Section: Introductionmentioning

confidence: 99%

Biochemical and hemostatic description of a thrombin-like enzyme TLBro from Bothrops roedingeri snake venom

Vilca-Quispe,

Alvarez-Risco,

Gomes Heleno

et al. 2023

Front. Chem.

View full text Add to dashboard Cite

Objective: The current study’s objective is to characterize a new throm-bin-like enzyme called TLBro that was obtained from Bothrops roedingeris snake from a biochemical and hemostatic perspective.Methodology: One chromatographic step was used to purify it, producing the serine protease TLBro. Molecular mass was estimated by SDS-PAGE to be between reduced and unreduced by 35 kDa. Tryptic peptide sequencing using Swiss Prot provided the complete amino acid sequence. Expasy.org by conducting a search that is limited to Crotalinae snake serine proteases and displaying a high degree of amino acid sequence.Results: Ser (182) is inhibited by phenylmethylsulfonyl fluoride (PMSF), and TLBro demonstrated the presence of Asp (88) residues. It also deduced the positions of His (43) and Ser (182) in the set of three coordinated amino acids in serine proteases. It was discovered that this substrate had high specificity for BANA, Michaelis-Menten behavior with KM 0 point85 mM and Vmax 1 point89 nmoles -NA/L/min, and high stability between temperatures (15 to 70°C) and pHs (2 point0 to 10 point0). According to doses and incubation times, TLBro degraded fibrin preferentially on the B-chain; additionally, its activities were significantly diminished after preincubation with divalent ions (Zn2 and Cd2). When incubated with PMSF, a particular serine protease inhibitor, enzymatic activities and platelet aggregation were inhibited.Conclusion: The findings revealed distinct structural and functional differences between the serine proteases, adding to the information and assisting in the improvement of the structure-function relationship.

show abstract

Effect of hemocoagulase on the coagulation parameters, blood transfusion volume and bleeding volume of patients with severe traumatic fractures

2023

View full text Add to dashboard Cite

To investigate the effect of hemocoagulase on coagulation parameters, blood transfusion volume and bleeding volume in patients with severe traumatic fractures. A total of 116 patients with severe traumatic fractures admitted to our hospital. These patients were randomly divided into the study group (hemocoagulase injection) and control group (normal saline injection), with 58 patients in each group. The bleeding time, coagulation time, perioperative bleeding volume, blood transfusion volume, hematology and coagulation parameters of the two groups were observed and compared. At 24 h post-surgery, the bleeding time and coagulation time were significantly shorter in the study group than in the control group (p < 0.05). The intraoperative bleeding volume, 24 h postoperative drainage volume, and blood transfusion volume were significantly lower in the study group than in the control group (p < 0.05). In addition, the 24 h postoperative levels of hematology parameters were significantly higher in the study group than in the control group (p < 0.05). However, coagulation parameters were similar in both groups before and at 24 h after surgery. Hemocoagulase has a better hemostatic effect and can effectively reduce perioperative bleeding without affecting the coagulation functions of patients with severe traumatic fractures. Therefore, hemocoagulase is a reliable treatment regimen in clinical practice.

show abstract

Production of a murine mAb against Bothrops alternatus and B. neuwiedi snake venoms and its use to isolate a thrombin-like serine protease fraction

Cited by 3 publications

References 41 publications

Effect of Seaweed-Derived Fucoidans from Undaria pinnatifida and Fucus vesiculosus on Coagulant, Proteolytic, and Phospholipase A2 Activities of Snake Bothrops jararaca, B. jararacussu, and B. neuwiedi Venom

Effect of Seaweed-Derived Fucoidans from Undaria pinnatifida and Fucus vesiculosus on Coagulant, Proteolytic, and Phospholipase A2 Activities of Snake Bothrops jararaca, B. jararacussu, and B. neuwiedi Venom

Biochemical and hemostatic description of a thrombin-like enzyme TLBro from Bothrops roedingeri snake venom

Effect of hemocoagulase on the coagulation parameters, blood transfusion volume and bleeding volume of patients with severe traumatic fractures

Contact Info

Product

Resources

About