Production of a root‐specific flavour compound, 2‐hydroxy‐4‐methoxy benzaldehyde by normal root cultures of <i>Decalepis hamiltonii</i> Wight and Arn (Asclepiadaceae)

Giridhar, P.; Rajasekaran, T.; Ravishankar, G. A.

doi:10.1002/jsfa.1939

Cited by 18 publications

(4 citation statements)

References 10 publications

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“…The extract was found to contain flavor compound 2-hydroxy-4-methoxy benzaldehyde (2H4MB), which was identified by TLC [22]. The extracts were analyzed for 2H4MB content by spotting the root extracts on TLC plate along with standard.…”

Section: Resultsmentioning

confidence: 99%

Study of Bioactive components in Decalepis hamiltonii invitro

S¹

2014

iosrphr

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Section: Resultsmentioning

confidence: 99%

Study of Bioactive components in Decalepis hamiltonii invitro

S¹

2014

iosrphr

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“…Also studies to some extent substantiated this plant tuber's usage in traditional medicine as an appetizer and blood purifier and also as an edible in making recipes (Pradeep et al 2016a). Similarly, through biotechnological intervention, effective in vitro propagation methods and alternate mode of flavour attributing 2H4MB production by using in vitro root cultures was reported (Giridhar et al 2005;Giridhar 2016, 2017). In view of the increasing demand for the tubers of D. hamiltonii, there is a concern on its survivability and existence as the local ethnic communities yank the tubers through destructive harvest method for economic gains.…”

Section: Introductionmentioning

confidence: 86%

Enhanced production of vanillin flavour metabolites by precursor feeding in cell suspension cultures of Decalepis hamiltonii Wight & Arn., in shake flask culture

2017

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The flavour rich tuberous roots of are known for its edible and medicinal use and have become endangered due to commercial over-exploitation. Besides 2-Hydroxy-4-methoxy benzaldehyde (2H4MB), other flavour metabolites in tuberous roots include vanillin, 4-Methoxy Cinnamic acid derivatives, aromatic alcohols etc. So far, there are no reports on the pathway of 2H4MB biosynthesis nor there is an organized work on biotransformation using normal and cell suspension cultures for obtaining these metabolites using precursors. The main aim of the study is to develop a method for enhanced production of flavour attributing metabolites through ferulic acid (FA) feeding to the callus culture medium. Biomass of cell suspension cultures was maximum (200.38 ± 1.56 g/l) by 4th week. Maximum production of 2H4MB was recorded on 4th week (0.08 ± 0.01 mg/100 g dry weight) as quantified by HPLC. Addition of 0.1-1.5 mM ferulic acid as precursor in the culture medium showed significant ( < 0.001) effect on suspension cultures biomass and respective phenylpropanoid metabolites content and 2H4MB accumulation. The maximum accumulation of vanillin, 2H4MB, vanillic acid, ferulic acid were of 0.1 ± 0.02 mg/100 g, 0.44 ± 0.01 mg/100 g, 0.52 ± 0.04 mg/100 g, 0.18 ± 0.02 mg/100 g DW respectively in 4 weeks of cultured cells supplemented with 1 mM ferulic acid as a precursor. The results indicate that, substantial increase in the levels of flavour metabolites in callus suspension culture was achieved. This would be having implications in biosynthesis of respective vanilla flavour attributing metabolites at very high levels for their large scale production.

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“…Micropropagation studies on D. arayalpathra were done [7,8] and analyzed MBALD in normal root cultures of this plant. According to the work reported the allied species, D. hamiltonii also produce MBALD [9]. In vitro secondary metabolite production is enhanced through the application of a variety of elicitors to plant cell or tissue culture systems.…”

Section: Introductionmentioning

confidence: 99%

In vitro Secondary Metabolite Production from the Roots of Decalepis arayalpathra KMA 05 Clones and its Antimicrobial Potential using Methylobacterium sp. VP103 as an Elicitor

Srivastava¹,

Laisram²,

Singh³

2018

Eur J Exp Bio

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Decalepis arayalpathra KMA 05 clones were elicited with a bacterium, Methylobacterium sp. VP103 isolated from the leaves of ex-vitro established plantlets of D. arayalpathra KMA 05 clones, with the aim of increasing the production of root specific secondary metabolites. D. arayalpathra KMA 05 shoot tip cultures were triggered for root initiation by keeping the cultures for one month in ½ strength Woody Plant Medium (WPM)+Thidiazuron (TDZ) 0.3 mg/l.They were then shifted to ½ strength WPM supplemented with auxins [0.5 mg/l indole-3-butyric acid (IBA) + 0.05 mg/l Naphthalene acetic acid (NAA)] along with 40 mg/l activated charcoal with a fixed concentration (0.4 mg/l) of lyophilized bacterium powder (LBP) dissolved in 30% methanol.The effect of elicitation was studied on three root specific secondary metabolites, i.e. 2-hydroxy 4-methoxy benzaldehyde (MBALD), 2-methoxy-4-vinylphenol (2M4VP) and α-amyrin. The production of α-amyrin was found to be enhanced in all 12, 24 and 36 week old treated root extracts. Antibacterial activity was also found to be enhanced against all the bacteria selected from the 36 week elicited extracts among which Rhodococcus sp. UKS7 (34 ± 0.0 mm.) showed maximum inhibition. The findings provide an insight into the use of Methylobacterium sp. VP103 as an elicitor in the growth of the roots and enhanced production of α-amyrin.

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Production of a root‐specific flavour compound, 2‐hydroxy‐4‐methoxy benzaldehyde by normal root cultures of Decalepis hamiltonii Wight and Arn (Asclepiadaceae)

Cited by 18 publications

References 10 publications

Study of Bioactive components in Decalepis hamiltonii invitro

Study of Bioactive components in Decalepis hamiltonii invitro

Enhanced production of vanillin flavour metabolites by precursor feeding in cell suspension cultures of Decalepis hamiltonii Wight & Arn., in shake flask culture

In vitro Secondary Metabolite Production from the Roots of Decalepis arayalpathra KMA 05 Clones and its Antimicrobial Potential using Methylobacterium sp. VP103 as an Elicitor

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