Production of a xylose-stimulated β-glucosidase and a cellulase-free thermostable xylanase by the thermophilic fungus Humicola brevis var. thermoidea under solid state fermentation

Masui, Douglas Chodi; Zimbardi, Ana Lucia Ribeiro Latorre; Souza, Flávio Henrique Moreira; Guimarães, Luís Henrique Souza; Furriel, Rosa dos Prazeres Melo; Jorge, João Atı́lio

doi:10.1007/s11274-012-1079-1

Cited by 39 publications

(20 citation statements)

References 60 publications

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“…Wheat bran is a substrate with good water absorption capacity, and its composition favors microbial cultivation for enzymes production (OLIVEIRA et al, 2015). Previous studies have reported the production of cellulases and hemicellulases by solid-state fermentation in wheat bran by several microorganisms (RODRIGUEZ-ZUNIGA et al, 2011;DELABONA et al, 2012;MASUI, et al, 2012;MITTAL, et al, 2013;GOWDHAMAN et al, 2014;PIROTA et al, 2015). Thus, wheat bran was used as substrate for T. aurantiacus cultivation in subsequent steps.…”

Section: Resultsmentioning

confidence: 99%

“…This catalytic property enables these enzymes to be used in the hydrolysis of vegetal biomass for the production of biofuels, the formulation of animal feed, or even in bread-making processes to improve the quality of dough, hydrolyzing xylooligosaccharides that are disadvantageous to the formation of gluten and impairs the crumb structure (BECKER et al, 2009;BRIENZO et al, 2012;OLIVEIRA et al, 2014;PIROTA et al, 2015;TERRASAN and CARMONA, 2015). However, the biobleaching of kraft pulp during the processing of cellulose for paper production is the more widespread and desired use of xylanolytic enzymes, due of the economic importance of this industrial segment (MASUI et al, 2012;MITTAL et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…An alternative for reducing the final cost of these biomolecules involves the use of agro-industrial residues in solidstate fermentation to produce microbial enzymes (BRIENZO et al, 2012;MASUI et al, 2012;SILVA et al, 2013;TERRASAN and CARMONA, 2015). Approximately 40% of the final cost of an enzyme is derived from the medium used for microorganism cultivation (ROMERO et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Production of xylanase by a new strain of Thermoascus aurantiacus: obtainment of enzymatic extract with reduced cellulolytic activity for application in pulp and paper industries

Costa¹,

Scalabrini²,

Silvestre³

et al. 2016

Biosci. J.

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ABSTRACT:Xylanases are useful in several industrial segments, including pulp and paper bleaching, animal feed, and bread-making processes. However, the industrial use of these enzymes is closely related to its production cost and its catalytic properties. The process of solid state fermentation enables the use of agro-industrial residues as substrates for microbial cultivation and enzymes production, reducing costs. In the present study, different cultivation parameters were evaluated for the xylanase production by the thermophilic fungus Thermoascus aurantiacus, by solid state fermentation, using agro-industrial residues as substrates. High production of xylanase (1701.9 U g -1 of dry substrate) was obtained using wheat bran containing 65% of initial moisture, at 120 h of cultivation, and 45°C. The xylanase showed optimal activity at pH 5.0 and 75°C; its stability was maintained at pH 3.0-11.0. The enzyme retained its catalytic potential after 1 h, at 75°C. The enzymatic extract produced under optimized conditions showed reduced activities of endoglucanase and FPase. Our results, including the xylanase production by T. aurantiacus in low-cost cultivation medium, high structural stability of the enzyme, and reduced cellulolytic activity, encourage the application of this enzymatic extract in pulp and paper bleaching processes.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Production of xylanase by a new strain of Thermoascus aurantiacus: obtainment of enzymatic extract with reduced cellulolytic activity for application in pulp and paper industries

Costa¹,

Scalabrini²,

Silvestre³

et al. 2016

Biosci. J.

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show abstract

“…Several authors have reported thermostable xylanases obtained from thermophilic fungi, such as, Humicola brevis var. thermoidea (Masui et al, 2012), Paecilomyces themophila J18 (Yang et al, 2006) and T. aurantiacus RCKK (Jain et al, 2015). Most of these fungal xylanases thermostable belong to GH10 and GH11 family, and enzymes belonging to the same family, they present the same structure and amino acid sequence, and thus the thermal stability also did not differ (Collins et al, 2005;You et al, 2010).…”

Section: Thermostable Xylanase and Its Propertiesmentioning

confidence: 99%

“…The hemicellulose is a heteropolysaccharide component of the plant wall; it primarily comprises xylan, whose main chain is composed of residues of D-xylose joined by (1 → 4)-β-glycosidic linkages and the side chains by Larabinofuranose, D-glucuronic acid or 4-O-methyl-Dglucuronic acid (Wakiyama et al, 2008;Adsul et al, 2011). The complete depolymerization of xylan requires the synergic action of several enzymes: endo-β-1,4-xylanase (EC 3.2.1.8) and β-xylosidases (EC 3.2.1.37) to hydrolyze the main chain; acetylxylan esterases (EC 3.1.1.72), α-L-arabinofuranosidases (EC 3.2.1.55), ρ-coumaric acid esterases (EC 3.1.1.-), α-glucuronidases (EC 3.2.1.139), and feruloyl esterase (EC 3.1.1.7-) to act on the side chains (Masui et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Thermostable xylanase from thermophilic fungi: Biochemical properties and industrial applications

Torre¹,

Kadowaki²

2017

Afr. J. Microbiol. Res.

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Filamentous fungi have been investigated as producer of xylanases with relevant characteristics for application in different industrial sectors, such as bakery, beverage, biofuel, textile, animal feed, pharmaceutical, pulp and paper. Thus, this review will focus on biochemical properties and industrial use of thermostable xylanases produced by different filamentous fungi, as well as mechanisms of adaptation of thermophilic organisms to tolerate in high-temperature environments. These enzymatic properties of thermal and pH stability are crucial, especially in processes such as the manufacture of animal feed, pulp and paper industry. Reports on cha nges in enzyme structure, such as site -directed mutagenesis, insertion or substitution of amino acids, addition of disulfide bonds in the alpha helix or beta-sheet structure for improving the thermal stability will also be reported. However, strains of Thermomyces lanuginosus has been described as good producers of thermostable xylanases, as well as promising enzymes, because it does not require any change in structure to increase the tolerance to high temperatures.

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Improvement of cellulase and xylanase production by solid‐state fermentation of Stachybotrys microspora

Abdeljalil

Saibi

Hmad

et al. 2014

Biotech and App Biochem

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The current study investigated the production of cellulases and xylanases from the rare fungus Stachybotrys microspora under solid-state fermentation (SSF) on wheat bran (WB). A comparison of both activities was first performed in submerged cultures using various concentrations of WB, glucose, and cellulose as substrates. The maximal activity of β-glucosidases and xylanases was obtained with 2% and 4% WB, respectively, whereas cellulose yielded the highest endoglucanase production. The SSF conditions were therefore consequently optimized. A moisture content of 70% gave the most significant levels of enzyme production. Inoculation by spores led to better results than by preculture, with 10(5) spores per gram of dried matter as the best inoculum dose for all activities tested. Interestingly, the WB-based medium need not to be supplemented by an exogeneous nitrogen source. Considering the richness of S. microspora secreted proteins as lytic hydrolases, the crude extracellular enzyme extracts were successfully tested in two different biotechnological fields: protoplasting of fungi and subsequent extraction of their DNA, paper pulp hydrolysis to produce fermentable sugars.

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Production of a xylose-stimulated β-glucosidase and a cellulase-free thermostable xylanase by the thermophilic fungus Humicola brevis var. thermoidea under solid state fermentation

Cited by 39 publications

References 60 publications

Production of xylanase by a new strain of Thermoascus aurantiacus: obtainment of enzymatic extract with reduced cellulolytic activity for application in pulp and paper industries

Production of xylanase by a new strain of Thermoascus aurantiacus: obtainment of enzymatic extract with reduced cellulolytic activity for application in pulp and paper industries

Thermostable xylanase from thermophilic fungi: Biochemical properties and industrial applications

Improvement of cellulase and xylanase production by solid‐state fermentation of Stachybotrys microspora

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