2011
DOI: 10.1155/2011/283747
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Production of Active Nonglycosylated Recombinant B‐Chain of Type‐2 Ribosome‐Inactivating Protein from Viscum articulatum and Its Biological Effects on Peripheral Blood Mononuclear Cells

Abstract: Type-2 ribosome-inactivating proteins, composed of a toxic A-chain and lectin-like B-chain, display various biological functions, including cytotoxicity and immunomodulation. We here cloned the lectin-like B-chain encoding fragment of a newly identified type-2 RIP gene, articulatin gene, from Viscum articulatum, into a bacterial expression vector to obtain nonglycosylated recombinant protein expressed in inclusion bodies. After purification and protein refolding, soluble refolded recombinant articulatin B-chai… Show more

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Cited by 2 publications
(4 citation statements)
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“…The rATB bound leucocytes with preferential binding to monocytes and in in-vitro-stimulated peripheral blood mononuclear cells (PBMCs) to release cytokines without cytotoxicity, implicating B-chain fragment as a potential immunomodulatory. [84] Articulatin-D, a type II RIP, isolated from V. articulatum parasitic on Dalbergia sp., B-chain of this protein facilitates entry of protein into cells where A-chain inhibits translation attributing cytotoxicity of the protein. [85] Anti-inflammatory activity Ethnopharmacologically, plant is well known for the treatment of bone disorder, sprain, gout and bone fracture in many communities in India, Nepal and China.…”
Section: Nephroprotective Activitymentioning
confidence: 99%
See 1 more Smart Citation
“…The rATB bound leucocytes with preferential binding to monocytes and in in-vitro-stimulated peripheral blood mononuclear cells (PBMCs) to release cytokines without cytotoxicity, implicating B-chain fragment as a potential immunomodulatory. [84] Articulatin-D, a type II RIP, isolated from V. articulatum parasitic on Dalbergia sp., B-chain of this protein facilitates entry of protein into cells where A-chain inhibits translation attributing cytotoxicity of the protein. [85] Anti-inflammatory activity Ethnopharmacologically, plant is well known for the treatment of bone disorder, sprain, gout and bone fracture in many communities in India, Nepal and China.…”
Section: Nephroprotective Activitymentioning
confidence: 99%
“…This recombinant articulatin B‐chain (rATB) showed lectin activity specifically towards galactoside moiety. The rATB bound leucocytes with preferential binding to monocytes and in in‐vitro ‐stimulated peripheral blood mononuclear cells (PBMCs) to release cytokines without cytotoxicity, implicating B‐chain fragment as a potential immunomodulatory . Articulatin‐D, a type II RIP, isolated from V. articulatum parasitic on Dalbergia sp ., B‐chain of this protein facilitates entry of protein into cells where A‐chain inhibits translation attributing cytotoxicity of the protein …”
Section: Introductionmentioning
confidence: 99%
“…Besides the European mistletoe, extracts of the Korean and Chinese mistletoes ( Viscum album coloratum and Viscum articulatum , respectively) contain type-2 RIPs that bind D-galactose [20, 21] and have high structural homology with ML. Like ML-I, they are endowed with immunomodulatory properties, as demonstrated by in vitro and in vivo studies.…”
Section: Overview Of Immunomodulatory Plant Lectinsmentioning
confidence: 99%
“…Macrophages stimulation with KLM results in upregulation of TLR4 expression and enhanced TNF-α production, which is reduced by anti-TLR4 specific antibodies or by assaying macrophages from TLR-4 deficient mice [24]. The recombinant B-chain of the Chinese mistletoe lectin (articulatin) stimulates human mononuclear cells to release TNF-α and IL-6 [21]. This suggests that the B-chain acts as an immunomodulator, like that of the European and Korean mistletoes.…”
Section: Overview Of Immunomodulatory Plant Lectinsmentioning
confidence: 99%