Production of alkaline protease from a newly isolated Exiguobacterium profundum BK-P23 evaluated using the response surface methodology

Anbu, Periasamy; Annadurai, G.; Hur, Byung‐Ki

doi:10.2478/s11756-013-0159-5

Cited by 19 publications

(9 citation statements)

References 23 publications

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“…This optimum temperature for protease production was in consistent with Bacillus sp. MIG ( Gouda, 2006 ) and E. profundum BK-P23 ( Anbu et al., 2013 ). The protease production was decreased considerably when the temperature increased above 37 °C.…”

Section: Resultsmentioning

confidence: 99%

Production and partial characterization of dehairing alkaline protease from Bacillus subtilis AKAL7 and Exiguobacterium indicum AKAL11 by using organic municipal solid wastes

Hakim

Bhuiyan

Iqbal

et al. 2018

Heliyon

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Alkaline proteases have applications in numerous industries. In this study, we have isolated and screened proteolytic bacteria from poultry wastes mixed soil and identified two bacterial isolates as Bacillus subtilis AKAL7 and Exiguobacterium indicum AKAL11 based on 16S rDNA sequencing. Maximum level of protease production was achieved after 24 h of fermentation in a basal medium. The optimal temperature, initial pH of the media and agitation for alkaline protease production by these two isolates were 30 °C, pH 9.0 and 120 rpm, respectively. The both bacterial isolates produced maximum level of protease with 3.0% organic municipal solid wastes (OMSW) as the sole source of carbon and nitrogen under previously optimized fermentation conditions. In comparison with the shake flask, protease production increased about 2.5-fold in the bioreactor with reduction in fermentation period. The partial purification of protease resulted in a final 45.67 and 34.86-fold purified protease with a specific activity of 8335.34 and 9918.91 U/mg protein and a typical yield of 9.75 and 9.41% from B. subtilis and E. indicum, respectively. The optimum temperature and pH of the partially purified protease from the both sources was 40 °C and pH 9.0, respectively. Protease from the both isolates was stable at pH 7.0–12.0 and at temperatures up to 50 °C. The effects of protease inhibitors indicated that the protease from B. subtilis might be serine and cysteine type and from E. indicum might be cysteine type. Mg2+, K+ and Ca2+ stimulated but Zn2+, Hg2+, Co2+ and Fe3+ strongly inhibited the protease activity. The partially purified protease from B. subtilis substantially dehaired cow skin and decomposed gelatinous compound from X-ray film. Our study revealed that OMSW can be used as raw material for production of bacterial extracellular protease and alkaline protease from B. subtilis might be potential for industrial and biotechnological applications.

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Section: Resultsmentioning

confidence: 99%

Production and partial characterization of dehairing alkaline protease from Bacillus subtilis AKAL7 and Exiguobacterium indicum AKAL11 by using organic municipal solid wastes

Hakim

Bhuiyan

Iqbal

et al. 2018

Heliyon

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“…The optimum temperature of the protease from Exiguobacterium profundum BK-P23 isolated from soil in South Korea was 40 °C and the enzyme was stable at 50 °C [23]. The crude protease of marine Exiguobacterium sp.…”

Section: Effects Of Temperature Ph and Naclmentioning

confidence: 99%

“…SKPB5 [21]. The molecular weight of protease from Exiguobacterium profundum BK-P23 was estimated to be 52 kDa [23].…”

Section: Protease Purificationmentioning

confidence: 99%

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Purification and characterization of a new neutral metalloprotease from marine Exiguobacterium sp. SWJS2

Lei

Cui

Zhao

et al. 2015

Biotech and App Biochem

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Among the protease-producing bacterial strains isolated from deep-sea sediments, SWJS2 was finally selected and identified as genus Exiguobacterium. Plackett-Burman and orthogonal array designs were applied to optimize the fermentation conditions, and the results are as follows: Glucose 5g, yeast extract 15g, glycerin 2g and CaCl2 ⋅2H2 O 0.5 g dissolved in 1 L artificial seawater; temperature 25 °C, original pH 7, inoculum rate 2%, seed age 12 H, loading volume 25 mL (250-mL Erlenmeyer flask), shaking speed 150 rpm, and fermentation time 44 H. The protease activity production was improved from about 80 to 660 U/mL under the optimized parameters. The protease was purified fourfold with specificity activity of 30,654.1 U/mg protein and a total yield of 16.2%. The protease exhibited the maximum activity at 40-45 °C and pH 7. Moreover, the enzyme activity was found to be inhibited by Cu(2+) , Ba(2+) , Cd(2+) , Hg(2+) , and Al(3+) at 5 mM, whereas it can be increased by Mg(2+) , Mn(2+) , and Ca(2+) at 0.5-5 mM. The enzyme was totally inactivated by 1 or 5 mM ethylenediaminetetraacetic acid but not by phenylmethanesulfonyl fluoride, tyrpsin inhibitor from Glycine max (STI), benzamidine, 5,5'-dithio-bis-(2-nitro benzoic acid), or pepstatin A, suggesting that it belonged to metalloprotease.

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“…Members of Exiguobacterium have been isolated from hypersaline tropical soils. These bacteria are able to grow at high pH and considered as good source of alkaliphilic enzymes such as proteases, lipases, cellulases and gelatinase [42,46].…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Haloalkaliphilic Bacteria Isolated from the Rhizosphere of Dichanthium annulatum

Mukhtar¹,

Malik²,

Mehnaz³

2018

JARB

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Hypersaline environments are widely distributed across the globe as salt mines, saline lakes, salt marshes and marine water [1, 2]. Halophytes such as Atriplex, Salsola, Dichanthium, kallar grass and para grass may contribute significantly to the developing world's supply of food, fiber, fuel and fodder. For areas where farm land has been salinized by poor irrigation practices or that overlie reservoirs of brackish water or for coastal desert regions, these plants could be successfully grown [3, 4]. The rhizosphere of halophytes harbors an impressive array of halophilic and alkaliphilic microorganisms. Poly extremophilic organisms have ability to tolerate two or more extreme conditions, such as haloalkaliphiles, halothermophiles and alkalithermophiles [5]. Haloalkaliphiles are organisms that require high salinity (3-30%) and an alkaline pH (pH 9-13) for their growth [6]. These organisms have been isolated and characterized from a number of environments such as saline

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Production of alkaline protease from a newly isolated Exiguobacterium profundum BK-P23 evaluated using the response surface methodology

Cited by 19 publications

References 23 publications

Production and partial characterization of dehairing alkaline protease from Bacillus subtilis AKAL7 and Exiguobacterium indicum AKAL11 by using organic municipal solid wastes

Production and partial characterization of dehairing alkaline protease from Bacillus subtilis AKAL7 and Exiguobacterium indicum AKAL11 by using organic municipal solid wastes

Purification and characterization of a new neutral metalloprotease from marine Exiguobacterium sp. SWJS2

Isolation and Characterization of Haloalkaliphilic Bacteria Isolated from the Rhizosphere of Dichanthium annulatum

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