Production of amelogenin by enamel epithelium of Hertwig's root sheath

Hamamoto, Yoshioki; Nakajima, Tamio; Ozawa, Hiroki; Uchida, Takashi

doi:10.1016/s1079-2104(96)80077-1

Cited by 55 publications

(61 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These epithelial cells appeared enlarged and produced amelogenin and ameloblastin even though enamel protein detection is technically difficult in postnatal periodontium at the basal level 41,61 . Evident enamel protein detection in epithelial root Malassez cells is related to physiopathological situations such as inflammation, 62 cementum repair and, here, periodontal defects in Msx2 null mice. Alterations to dental hard tissues may have consequences in mastication function and could contribute to these periodontal defects.…”

Section: Msx2mentioning

confidence: 94%

Enamel Protein Regulation and Dental and Periodontal Physiopathology in Msx2 Mutant Mice

Molla

Descroix

Aïoub

et al. 2010

The American Journal of Pathology

View full text Add to dashboard Cite

Signaling pathways that underlie postnatal dental and periodontal physiopathology are less studied than those of early tooth development. Members of the muscle segment homeobox gene (Msx) family encode homeoproteins that show functional redundancy during development and are known to be involved in epithelial-mesenchymal interactions that lead to crown morphogenesis and ameloblast cell differentiation. This study analyzed the MSX2 protein during mouse postnatal growth as well as in the adult. The analysis focused on enamel and periodontal defects and enamel proteins in Msx2-null mutant mice. In the epithelial lifecycle, the levels of MSX2 expression and enamel protein secretion were inversely related. Msx2؉/؊ mice showed increased amelogenin expression, enamel thickness, and rod size. Msx2 ؊/؊ mice displayed compound phenotypic characteristics of enamel defects, related to both enamel-specific gene mutations (amelogenin and enamelin) in isolated amelogenesis imperfecta, and cell-cell junction elements (laminin 5 and cytokeratin 5) in other syndromes. These effects were also related to ameloblast disappearance, which differed between incisors and molars. In Msx2 ؊/؊ roots, Malassez cells formed giant islands that overexpressed amelogenin and ameloblastin that grew over months. Aberrant expression of enamel proteins is proposed to underlie the regional osteopetrosis and hyperproduction of cellular cementum. These enamel and periodontal phenotypes of Msx2 mutants constitute the first case report of structural and signaling defects associated with enamel protein overexpression in a postnatal context.

show abstract

Section: Msx2mentioning

confidence: 94%

Enamel Protein Regulation and Dental and Periodontal Physiopathology in Msx2 Mutant Mice

Molla

Descroix

Aïoub

et al. 2010

The American Journal of Pathology

View full text Add to dashboard Cite

show abstract

“…The function of ameloblastin in enamel as well as in root formation remains unknown. The absence of amelogenin expression is more intriguing since Hamamoto et al (1996) reported the capacity of HERS cells to produce enamel and express amelogenins in response to pulp inflammation. However, this finding was achieved under pathologic Table 1.…”

Section: Hers Express Enamel Proteinsmentioning

confidence: 99%

Role of Hertwig's epithelial root sheath cells in tooth root development

Zeichner‐David

Oishi

et al. 2003

Developmental Dynamics

207

176

View full text Add to dashboard Cite

During tooth development, after the completion of crown formation, the apical mesenchyme forms the developing periodontium while the inner and outer enamel epithelia fuse below the level of the crown cervical margin to produce a bilayered epithelial sheath termed Hertwig's epithelial root sheath (HERS). The role of HERS cells in root formation is widely accepted; however, the precise function of these cells remains controversial. Functions suggested have ranged from structural (subdivide the dental ectomesenchymal tissues into dental papilla and dental follicle), regulators of timing of root development, inducers of mesenchymal cell differentiation into odontoblasts and cementoblasts, to cementoblast cell precursors. The characterization of the HERS phenotype has been hindered by the small amount of tissue present at a given time during root formation. In this study, we report the establishment of an immortal HERS-derived cell line that can be maintained in culture and then induced to differentiate in vitro. Characterization of the HERS phenotype using reverse transcriptase-polymerase chain reaction and Western blot immunostaining suggests that HERS cells initially synthesize and secrete some enamel-related proteins such as ameloblastin, and then these cells appear to change their morphology and produce a mineralized extracellular matrix resembling acellular cementum. These studies suggest that the acellular and cellular cementum are synthesized by two different types of cells, the first one by HERS-derived cementoblasts and the later by neural crest-derived cementoblasts. Developmental Dynamics 228:651-663, 2003.

show abstract

“…In particular, CK14 stains odontogenic epithelium in all stages of tooth development, including the dental lamina and stellate reticulum, while CK19 is more prominent in later stages [2,3]. Many other molecular markers have been demonstrated during odontogenesis, particularly in relation enamel proteins and to transcription factors, which control the order and morphology of teeth [4,5]. However, no suggestion of a clinical use in diagnosis has been explored.…”

Section: Introductionmentioning

confidence: 99%

The Diagnostic Usefulness of Immunohistochemistry for Odontogenic Lesions

Hunter

Speight

2014

Head and Neck Pathol

View full text Add to dashboard Cite

The diagnosis of odontogenic tumors can be challenging, largely due to their rarity and consequent difficulties in gaining experience in their assessment. In most cases, careful attention to morphology, in conjunction with clinical and radiological features will allow a diagnosis to be made. However, in some cases, immunohistochemical analysis of the tumor may be useful. In this review we will outline the immunohistochemical expression profile of normal developing odontogenic tissues and a range of odontogenic tumors. In many cases the immunohistochemical markers are neither specific nor sensitive enough to be of help in diagnosis, but in some cases such analysis may prove very useful. Thus we have outlined a limited number of circumstances where immunohistochemistry may be of use to the practicing diagnostic pathologist.

show abstract

Production of amelogenin by enamel epithelium of Hertwig's root sheath

Cited by 55 publications

References 25 publications

Enamel Protein Regulation and Dental and Periodontal Physiopathology in Msx2 Mutant Mice

Enamel Protein Regulation and Dental and Periodontal Physiopathology in Msx2 Mutant Mice

Role of Hertwig's epithelial root sheath cells in tooth root development

The Diagnostic Usefulness of Immunohistochemistry for Odontogenic Lesions

Contact Info

Product

Resources

About