PRODUCTION of AMYLASE by A NOVEL Bacillus sp. ZBP10 in SUBMERGED FERMENTATION

Abstract

Bacillus sp. ZBP10 is an amylase producing strain isolated from a soil sample collected from a potato cultivation field in Sakarya. In this study, culture conditions were optimized for Bacillus sp. ZBP10 in order to increase amylase production using submerged fermentation. The effects of temperature (30-40°C), fermentation time (24-72 h), initial medium pH (6.0-9.0), carbon sources (soluble, wheat, rice and corn starches) and substrate concentration (5-30 g/L) on the production of amylase were determined. According to the results, the bacterium produced maximum amount of amylase, when the initial pH of the medium was 7.0, at 33°C, and within 48 h. Soluble starch was the best substrate among the starches tested. Optimum substrate concentration was 20 g/L for enzyme production with which 3.57±0.19 U/mL enzymatic activity was obtained.