Production of amylases from Bacillus amyloliquefaciens under submerged fermentation using some agro-industrial by-products

Abstract

Thirty-one bacterial isolates out of 133 isolates, were obtained from rhizosphere of Egyptian clover plants, and had variant capability for starch degradation on starch agar medium. The isolate E109 was the most potent being 72.5 U ml À1 and 2.5 for amylase activity and starch hydrolysis ratio (SHR), respectively, at 50°C. The potent isolate E109 was identified based on phenotypic characteristics, phylogenetic positions based on 16S rRNA gene analysis and base sequences (submitted to NCBI Gen Bank). 16S rRNA gene analysis confirmed that this isolate belonged to the genus Bacillus and it was most closely related to B. amyloliquefaciens (95% similarity). For the production of amylases, nine agro-industrial residues were added as carbon sources to the basal medium. The medium supplemented with potato starchy waste as the sole carbon source enhanced the enzyme activity more than soluble starch as control for a, b and c amylases activity, as it increased by B. amyloliquefaciens about 1.26 & 4 and 8-fold, respectively after 48 h at 50°C using rotary shaker at 150 rpm. B. amyloliquefaciens gave the maximum values of a, b and c amylases activity on medium supplemented with 2% potato starchy waste after 30, 30 & 36 h of fermentation periods at 50°C using shake flasks technique as a batch culture. These values were 155.2 U ml À1 (R 2 = 0.93), 1.0 U ml À1 (R 2 = 0.94) and 2.4 U ml À1 (R 2 = 0.95), respectively. It could be stated that productive medium supplemented with 2% potato starchy waste as a low price substrate could be more favorable than basal medium containing 1% starch for amylases production in submerged fermentation, as it increased a, b and c amylase activity by 1.98, 7.69 and 12-fold than that produced in basal medium (control), respectively. ª 2015 Production and hosting by Elsevier B.V. on behalf of Faculty of Agriculture, Ain Shams University.

Optimization of Thermo-Alkali Stable Amylase Production and Biomass Yield from Bacillus sp. Under Submerged Cultivation

“…The current investigation strongly agreed with the findings of Dash et al [13] who demonstrated significant amylase yield from Bacillus sp. at the end of 24 h. Conversely, Abd-Elhalem et al [5] demonstrated the maximum yield of amylase from Bacillus sp. during 36 h of incubation.…”

“…Our results totally agree with the findings of Samanta et al [12] and Karatas et al [13] who demonstrated maximum amylase production in the presence of starch. On the other hand, Deb et al [14] and Abd-Elhalem et al [5] had recorded maximum amylase production from Bacillus sp. in the presence of corn flour and different agro-industrial wastes respectively.…”

Biosynthesis of industrial enzymes by free and immobilized Alicyclobacillus in different matrices and the use of ultrafiltration in the enzymes concentration

“…Based on this result, wheat bran was selected for the production of the enzyme and to perform the subsequent Agroindustrial by-products have been evaluated as substrates for the production of amylase in order to reduce production costs through fermentation in solid state and submerged fermentation. Abd-Elhalem et al 27 used industrial waste in the production of the amylase enzyme by Bacillus amyloliquefaciens in submerged fermentation, and the residue from potato processing exhibited higher activity (80.1 U mL -1 ) than soluble starch (72.3 U mL -1 ). Amylase production from Bacillus cereus MTCC 1305 by solid state fermentation using wheat bran resulted in a greater production of this enzyme (94 U g -1 ) than by production with rice flakes (34 U g -1 )…”

Goat dung as a feedstock for hyper-production of amylase from Glutamicibacter arilaitensis strain ALA4

“…Recently, few investigations had already reported the overwhelming yield of amylases from distinct bacterial sources using unique organic wastes viz. cassava bagasse (Ray and Kar 2009), cow dung (Vijayaraghavan et al 2015), potato starchy wastes (Abd-Elhalem et al 2015), and organic chicken wastes (Hasan et al 2017). But study on the utilization of goat dung as feedstock for the successful production of amylase is not evidenced so far.…”