Production of an alkaline protease by Bacillus cereus MCM B-326 and its application as a dehairing agent

Abstract

The present investigation describes microbial production of an alkaline protease and its use in dehairing of buffalo hide. Bacillus cereus produced extracellular protease when grown on a medium containing starch, wheat bran and soya flour (SWS). The ammonium sulphate precipitated (ASP) enzyme was applied for dehairing of buffalo hide. Microscopic observation of longitudinal section of buffalo hide revealed that the epidermis was completely removed and hair was uprooted leaving empty follicles in the hide. The ASP enzyme was stable for one month at ambient temperature between 25-35°C. Enzymatic dehairing may be a promising shift towards an environment-friendly leather processing method.

Optimized production and properties of thermostable alkaline protease from Bacillus subtilis SHS-04 grown on groundnut (Arachis hypogaea) meal

“…I-312 in a medium containing wheat flour and soybean meal as the carbon and nitrogen sources. Furthermore, protease production from Bacillus cereus MCM B-326 using media containing deoiled groundnut cakes have been reported but with enzyme yield below 200 U/mL [28]. The protease production by B. subtilis SHS-04 using groundnut meal as nitrogen source in the present study has greatly enhanced protease production more than other nitrogen sources studied.…”

A novel serine alkaline protease from Bacillus altitudinis GVC11 and its application as a dehairing agent

“…3d, f). The ever-increasing attention to the environmental impact of leather industry has necessitated the development of enzyme-based processes as potent alternatives to pollution causing chemicals [25,29]. A significant feature of the enzymatic dehairing process is the complete hair removal and minimal usage of the sulphide.…”

Purification, characterization and application of novel alkaline protease from new Bacillus cereus UV-15 mutant

“…Among several bacterial strain, B. subtilis [9], B. licheniformis, B. amylolequifaciens, B. cereus [10] and Streptomyces sps. [11] have been characterized for dehairing purposes.…”