Production of androstenedione using mutants of Mycobacterium sp

Cholesterol oxidase ChoD is not a critical enzyme accounting for oxidation of sterols to 3-keto-4-ene steroids in fast-growing Mycobacterium sp. VKM Ac-1815D

“…Ac-1815D is a strain effectively oxidizing sterols (phytosterol, cholesterol, ergosterol) to androstenedione and other valuable steroids [24,25,39,40]. Based on 3-oxo-4-ene structure of the metabolites and intermediates, it was supposed that ChO can be involved in the multienzyme sterol oxidation in this strain.…”

“…Fast growing mycobacteria are naturally resistant to many antibiotics [39]. When choosing an appropriate antibiotic resistant marker for positive selection of allelic exchange, we tested the sensitivity of Ac-1815D strain to more than 20 antibacterial agents.…”

Excretion of endogenous boldione in human urine: Influence of phytosterol consumption

“…Biotransformation of phytosterols to steroid hormones has thus far not been demonstrated in humans while microbial conversion of phytosterols to steroids has been frequently reported [25][26][27][28][29][30][31]. In addition, a number of studies has been devoted to the ability of invertebrate organisms to convert phytosterols into anabolic steroids [32][33][34].…”

From Soybean Phytosterols to Steroid Hormones

“…The fundamental solution is to develop robust, efficient, highly productive microbial strains. Random mutagenesis is the conventional breeding approach and has been the main means of developing custom phytosterol-transforming strains (Egorova, et al, 2002;Donova, et al, 2005a;Sripalakit, et al, 2006). However, strains surviving multiple rounds of mutation are genetically undefined and vulnerable to further changes.…”

“…Most phytosterol-transforming strains can transform phytosterols into many structurally similar products, such as the C19 steroids AD, ADD, and their derivatives, at the same time (Egorova, et al, 2002;Donova, et al, 2005aDonova, et al, , 2005bDonova, et al, , 2005cMolchanova, et al, 2007). Because the differences in the structures of these products are very subtle, it is difficult to separate and purify them.…”