Production of anti-HIV-1 calanolides in a callus culture of Calophyllum brasiliense (Cambes)

Bernabé-Antonio, Antonio; Estrada-Zúñiga, María Elena; Buendía-González, L.; Reyes-Chilpa, Ricardo; Chávez-Ávila, Víctor Manuel; Cruz-Sosa, Francisco

doi:10.1007/s11240-010-9750-4

Cited by 32 publications

(22 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Studies of secondary metabolism using in vitro cultures are useful as they allow for controlling enzymes and/or substrates involved in synthesis of metabolites (Sood and Chauhan 2010;Bernabe-antonio et al 2010;Pati et al 2011;Nasim et al 2011). Although many cyclopeptides have been isolated from various plants, the biosynthetic mechanism for the majority of the peptides remains unclear.…”

Section: Resultsmentioning

confidence: 99%

An in vitro system to study cyclopeptide heterophyllin B biosynthesis in the medicinal plant Pseudostellaria heterophylla

Zhu

Tan

et al. 2011

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Plant cyclopeptides are a large group of small molecule metabolites found in a wide variety of plants, including traditional Chinese medicinal plants. Many of the cyclopeptides have highly unusual structures and potent biological activities. However, the majority of the cyclopeptides have not been studied for their biosynthetic mechanisms. In this study, we have established a culture system for the biosynthetic study of heterophyllin B (HB), a cyclopeptide produced by the medicinal plant Pseudostellaria heterophylla. We first developed a shoot culture of P. heterophylla that produced HB consistently under laboratory conditions. Using 14 C-labeled proline as tracer, we showed that labeled HB was produced by the cultured shoots, indicating that this system has de novo biosynthetic activity. Next, we chemically synthesized HB's linear peptide precursor (LHB) and the N-acetyl cysteamine thioester of LHB (LHB-SNAC). When LHB-SNAC was incubated with total cell free extracts of the cultured shoots, a small amount of cyclized product (HB), in addition to the hydrolyzed product (LHB), was produced. The in vivo and in vitro results demonstrate the presence of an HB biosynthetic system, which provides insight into the molecular mechanism for plant cyclopeptide biosynthesis.

show abstract

Section: Resultsmentioning

confidence: 99%

An in vitro system to study cyclopeptide heterophyllin B biosynthesis in the medicinal plant Pseudostellaria heterophylla

Zhu

Tan

et al. 2011

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…Trata-se de planta com alto potencial madeireiro e com boa possibilidade de cultivo, especialmente em áreas úmidas, com moderado excesso de água, próximas a cursos de água (Oliveira & Joly, 2010;Schultz, 2011). A espécie também tem importância farmacêutica, por possibilitar a extração de metabólitos derivados de xantonas, cumarinas, flavonoides e terpenos, que têm ação antibacteriana, moluscicida, anti-hipertensiva e atividade contra imunodeficiência humana (HIV-1) (Noldin et al, 2006;Braga et al, 2007;Bernabé-Antonio et al, 2010;Carvalho et al, 2013).…”

Section: Introductionunclassified

Técnicas para superação da dormência de sementes de guanandi

Silva

Vieira

Panobianco

2014

Pesq. agropec. bras.

View full text Add to dashboard Cite

Resumo -O objetivo deste trabalho foi avaliar tipos de preparo de sementes de guanandi (Calophyllum brasiliense), com vistas à superação da dormência física e mecânica, e verificar a influência da temperatura e do substrato sobre a germinação. Foram avaliados três substratos (papel, areia e vermiculita), duas temperaturas de germinação (25 e 30ºC) e quatro tipos de preparo das sementes: sementes íntegras; sementes nuas, sem tegumento e endocarpo; sementes com punctura no endocarpo, na região próxima ao eixo embrionário ou na região oposta ao eixo embrionário; e sementes cortadas a 1/3 da região oposta ao eixo embrionário. Foram realizados testes de vigor (índice de velocidade de germinação e emergência de plântulas em campo), e a curva de embebição foi obtida para os diferentes tipos de preparo da semente. A retirada total do envoltório (endocarpo e tegumento) é necessária para a completa superação da dormência física e mecânica das sementes de guanandi. A germinação das sementes deve ser realizada em substrato papel à temperatura de 30ºC.Termos para indexação: Calophyllum brasiliense, espécie nativa, germinação, madeira de lei, sementes recalcitrantes, testes laboratoriais. Techniques for overcoming dormancy in guanandi seedsAbstract -The objective of this work was to evaluate different types of preparation of guanandi (Calophyllum brasiliense) seeds, in order to overcome physical and mechanical dormancy, and to verify the effects of temperature and substrate on germination. Three substrates (paper, sand, and vermiculite), two germination temperatures (25 and 30ºC), and four seed preparation types were evaluated: whole seeds; bare seeds, without testa and endocarp; seeds with punctured endocarp in the region near the embryonic axis or in the region opposite of the embryonic axis; and seeds cut at 1/3 of the region opposite of the embryonic axis. Vigor tests were performed (index of germination speed and emergence of seedlings in the field), and the imbibition curve was obtained for the different types of seed preparation. Total wrap (endocarp and testa) withdrawal is necessary to completely overcome physical and mechanical dormancy of guanandi seeds. Seed germination should be held in paper substrate at a temperature of 30°C.

show abstract

“…(Clusiaceae), known in Brazil as guanandi, jacareuba or olandi, is a tree species native to tropical America, common in rainforests and humid soils, tolerant to hypoxia (Marques & Joly, 2000;Oliveira & Joly, 2010). This species is economically important due to the good quality of its wood (Cole et al, 2011), to its use for reforestation (Cole et al, 2011) and to its medicinal properties (Ito et al, 2002;Souza et al, 2009), including anti-HIV potential (Huerta-Reyes et al, 2004;Bernabé-Antonio et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

In vitro seed germination and multiplication of Calophyllum brasiliense

2016

View full text Add to dashboard Cite

Calophyllum brasiliense is a tree species with limited natural reproduction. In vitro germination may be an alternative for obtaining high-quality seedlings. Seeds were maintained in water before surface disinfestation and compared with control seeds (i.e. not immersed), without differences between treatments. HgCl2 used during surface-disinfestation reduced contamination rates of cultures. Fungal contamination was reduced with fungicide added to culture medium (23 to 6.4%), although bacterial contamination increased (24 to 36%). In another experiment, seeds were immersed in plant preservative mixture (PPM™) prior to surface disinfestation. By combining immersion for 48 h and 2 mL L-1 in culture medium, contamination was only 6%. Seeds immersion in GA3 prior to surface disinfestation reduced root formation as concentration increased. Germination rate and GSI were reduced, respectively, from 72% and 0.129 (24 h) to 60% and 0.092 (48 h) according to exposure time to GA3. After 90 days in multiplication medium containing benzylaminopurine, average number of shoots per nodal segment was 3.4. In conclusion, in vitro germination of C. brasiliense seeds is feasible in sucrose-free WPM medium and reaches a high contamination-free rate (up to 93.3%).

show abstract

Production of anti-HIV-1 calanolides in a callus culture of Calophyllum brasiliense (Cambes)

Cited by 32 publications

References 22 publications

An in vitro system to study cyclopeptide heterophyllin B biosynthesis in the medicinal plant Pseudostellaria heterophylla

An in vitro system to study cyclopeptide heterophyllin B biosynthesis in the medicinal plant Pseudostellaria heterophylla

Técnicas para superação da dormência de sementes de guanandi

In vitro seed germination and multiplication of Calophyllum brasiliense

Contact Info

Product

Resources

About