Production of anti‐inflammatory compounds in Sphaeralcea angustifolia cell suspension cultivated in stirred tank bioreactor

Abstract

Sphaeralcea angustifolia is a plant used for the treatment of inflammatory processes. Scopoletin, tomentin, and sphaeralcic acid were identified as the compounds with anti‐inflammatory and immunomodulatory effects. Successful establishment of the cell culture in Erlenmeyer flasks has been reported previously. The aim of this study was to evaluate the ability of cells in suspension from S. angustifolia grown in a stirred tank bioreactor and demonstrate their capacity to produce bioactive compounds. Cells in suspension grown at 200 rpm reached a maximal cell biomass in dry weight at 19.11 g/L and produced 3.47 mg/g of sphaeralcic acid. The mixture of scopoletin and tomentin was only detected at the beginning of the culture (12.13 μg/g). Considering that the profile of dissolved oxygen during the cultures was lesser than 15%, it is possible that the low growth at 100 rpm could be due to oxygen limitations or to cell sedimentation. At 400 rpm, a negative effect on cell viability could be caused by the increase in the hydrodynamic stress, including the impeller tip, average shear rate, and Reynolds number. The sphaeralcic acid content in the cell suspension of S. angustifolia obtained in the bioreactor was two orders of magnitude greater than that reported for the culture grown in Erlenmeyer flasks.

Elicitation-Based Method for Increasing the Production of Antioxidant and Bactericidal Phenolic Compounds in Dionaea muscipula J. Ellis Tissue

“…Moreover, hydromechanical stress in an LM system is connected with the intensity of culture' shaking. Perez-Hernandez et al [35] examined suspension cell culture of medical plant Sphaeralcea angustifolia and revealed that cells grown with 200 rpm had the highest cell biomass and increased concentration of sphaeralcic acid. Growing cells with 100 rpm induces oxygen deficit stress, while 400 rpm negatively affects viability of cells, which was the consequence of hydrodynamic stress.…”

Establishment and triterpenoid production of Ocimum basilicum cambial meristematic cells

“…The application of a suitable bioreactor system is crucial for plant cell cultivation in respect to biomass accumulation, cell viability and product formation (Eibl and Eibl 2008;Valdiani et al 2019). While stirred-tank reactors are routinely used for plant cell culture (Arias et al 2017;Pérez-Hernández et al 2019), the mechanical stirring poses a source of hydrodynamic stress for the plant cells, decreasing cell viability during the process (Takeda et al 1994). Alternatives without mechanical stirring are found in bubble column, airlift or some types of disposable bioreactors.…”

The Journey of a Medicinal Plant throughout Science: Sphaeralcea angustifolia (Cav.) G. Don (Malvaceae)

“…The maximum growth of the suspended cells (19.11 g/L) was recorded 11 days after culture at 200 rpm. Sphaeralcic acid was the primary intracellular compound whose content (52.15 mg/L) was significantly higher than that reported in cells developed in Erlenmeyer flasks [ 35 ].…”