2023
DOI: 10.24198/ijpst.v10i3.40641
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Production of Anti-Recombinant Human Insulin Antibody and Validation by Indirect ELISA

Christy Ambarsari,
Herman Suryadi,
Arry Yanuar

Abstract: Human insulin potential has become an interest and is important in maintaining the success of therapyin patients with the availability of chemical-based analytical methods, however, only a few have beenusing immunoassays. This study aimed to produce IgG polyclonal antibodies from rabbits immunizedwith 1 mg/mL rhINS subcutaneously and validated by indirect ELISA. Antibody was precipitated andfractioned on a HiTrap® Protein A HP column before being quantified with a UV spectrophotometerat λ 280 nm. The character… Show more

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