Production of Antibacterial and AntifungalMetabolites by (S.albovinaceus) Strain no.10/2 and MediaOptimization

Abdelghani, Thaer

doi:10.15640/aijb.v5n1a1

Cited by 8 publications

(8 citation statements)

References 17 publications

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“…The antibiotic biosynthesis of S. cavourensis YBQ59 was observed after 18 h and the antimicrobial activity against MRSE and S. Typhimurium was dramatically increased within 42 h, then slowly increased and reached a peak located between 72 h and 78 h (inhibition zones > 34.0 mm). The antimicrobial activity was stable until 90 h and then slightly decreased after 120 h. Thus, S. cavourensis YBQ59 produced a highest yield of antibiotics under the fermentation period between 72 h and 90 h. This result is consistent with previous reports [16,17] and suggests that the log phase is the best period for harvesting antibiotics produced by S. cavourensis YBQ59 under the optimal fermentation conditions. The CFSs of S. cavourensis YBQ59 was subjected to chemical analysis for the isolation of antimicrobial compounds (Table 1).…”

Section: Kinetic Of Fermentation Processsupporting

confidence: 90%

OPTIMAL FERMENTATION CONDITIONS FOR ANTIBIOTIC PRODUCTION BY ENDOPHYTIC Streptomyces cavourensis YBQ59 ISOLATED FROM Cinnamomum cassia Presl

Vu¹,

Nguyen²,

Le³

et al. 2019

JST

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This study determined the optimal nutrient and environmental conditions for the production of antimicrobial and cytotoxic agents by Streptomyces cavourensis YBQ59 during the fermentation process. The antimicrobial and cytotoxic activities of eluted fractions based gradient solvents via silica gel column of chromatograph were also evaluated. S. cavourensis YBQ59 exhibited strong, board-spectrum antimicrobial activities under fermentation conditions as follows: MT6 medium with soluble starch and soybean powder as carbon and nitrogen sources, respectively, temperature 30oC, initial pH 7.0, 20% DO concentration and with 5% initial seed culture. The kinetic of fermentation showed that the antimicrobial and cytotoxic activities were highest at between 72h and 78h. The fraction 25/1 D-M (dichloromethane –methanol) exhibited the highest antimicrobial effect against methicillin-resistant Staphylococcus aureus ATCC 33591 and methicillin-resistant Staphylococcus epidermidis ATCC 35984 with minimum inhibitory concentrations of 9.4 µg/ml and 6.4 µg/ml, respectively. The fraction 10/1 D-M had strong cytotoxic effects towards multidrug-resistant A459 and H1299 lung carcinoma cell lines with the cell viability of 11.3% and 12.4%, respectively. In conclusion, S. cavourensis YBQ59 would be a potential producer of valuable bioactive compounds that may have a board application in pharmaceutical industry and agriculture (biocontrol, livestock, food safety and quality management)

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Section: Kinetic Of Fermentation Processsupporting

confidence: 90%

OPTIMAL FERMENTATION CONDITIONS FOR ANTIBIOTIC PRODUCTION BY ENDOPHYTIC Streptomyces cavourensis YBQ59 ISOLATED FROM Cinnamomum cassia Presl

Vu¹,

Nguyen²,

Le³

et al. 2019

JST

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“…AS4. Another study by Abdelghani [ 49 ] also reported that malt extract concentration of 1.0% (w/v) showed maximum antibacterial yield in S. albovinaceus . A study of cell biomass and antibacterial activity of all isolates showed a positive correlation, which was the optimum antibacterial activity found in the incubation period of days 9 to 11 or when the growth reached the stationary phase (maximum cell biomass).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Streptomyces Isolates Associated with Estuarine Fish Chanos chanos and Profiling of Their Antibacterial Metabolites-Crude-Extract

Kurnianto

Kusumaningrum

Lioe

2020

International Journal of Microbiology

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Streptomyces has been reported as an essential producer of bioactive substances, including antibiotics and other types of antimicrobials. This study investigated antibacterial-producing Streptomyces isolated from the gut of estuarine fish Chanos chanos, emphasizing screening for the producer of peptide-containing antibacterial compounds. Eighteen isolates were found during preliminary screening, in which four isolates showed the best antibacterial activities. Based on the morphological, physiological, and biochemical characterization, as well as 16S rRNA partial sequencing, all of the four isolates belonged to Streptomyces. Three isolates were suspected as novel isolate candidates based on homology presentations and phylogenetic tree analysis. Disk-diffusion assay of the metabolite-crude-extract from the isolates showed broad-spectrum inhibitory activities against Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 10876, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa InaCC B52 with minimum inhibitory concentration and minimum bactericidal concentration ranging from 2.5–10 mg/mL and 5–10 mg/mL, respectively. The highest antibacterial activity with low MIC and MBC values was shown by isolate AIA-10. Qualitative HPLC profiling revealed that the metabolic-crude-extracts showed many peaks with intensive area at 210 and 214 nm, especially from SCA-11 and AIA-10, indicating the presence of peptide groups in the structure of the constituent compound. The results also suggested that crude extracts SCA-11 and AIA-10 had higher hydrophobicity properties than the other extracts. Further characterization of the active compound was needed to find out which compounds were responsible for the antibacterial activity. The results of this study indicated that some Streptomyces isolated from new environmental niches, i.e., gut of estuarine fish Chanos chanos, produce promising peptide-containing bioactive compounds.

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“…KADIRI & al [30]), fructose (S.N. KUMAR & al [20]), and mannitol (T. ABDELGHANI & al [31]) have been reported to be suitable for the activity of antibiotic substances in different microorganisms. These different results shows that the optimal carbon source must be identified for maximal yield of the antibiotic of interest in different bacteria.…”

Section: Resultsmentioning

confidence: 99%

Optimization of Culture Medium for the Production and Partial Purification and Characterization of an Antibacterial Activity from Brevibacillus laterosporus Strain EA62

Ak¹,

Demirkan²,

Cengiz³

et al. 2019

Rom Biotechnol Lett.

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The present study was performed to report the bacterial identification, optimization of culture conditions and characterization of a novel antibiotic substance from a Bacillus sp. EA62 strain isolated from soil. The EA62 strain was identified based on 16S rRNA analysis. The new isolate EBD 9-1 showed 100% sequence identity with Brevibacillus laterosporus. The antibacterial activity of the new substance was examined against five pathogenic bacteria and was observed to be the most effective against Escherichia coli. An agar diffusion assay was performed to evaluate the antibacterial activity of the substance. The effects of some nutritional (amino acid, carbon, nitrogen and metal sources) and physical factors (pH and temperature) and incubation time on the antibiotic activity were studied. Antibiotic activity in basal medium reached the maximum levels after 72 h of incubation. The best antibiotic activity was obtained in the presence of glucose as a carbon source, yeast extract as a nitrogen source, glutamic acid as an amino acid source and MgSO4+CaCO3 as metal ion sources. For physical parameters, the best results were obtained at 37°C, pH 7.0. The antibiotic substance was partially purified, and the estimated molecular weight was 6.3 kDa. The minimum inhibitory concentration (MIC) values determined against five pathogen bacteria were >256 µg/ml. The substance was identified by thin-layer chromatography, and its Rf value was measured as 0.04 cm.

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Production of Antibacterial and AntifungalMetabolites by (S.albovinaceus) Strain no.10/2 and MediaOptimization

Cited by 8 publications

References 17 publications

OPTIMAL FERMENTATION CONDITIONS FOR ANTIBIOTIC PRODUCTION BY ENDOPHYTIC Streptomyces cavourensis YBQ59 ISOLATED FROM Cinnamomum cassia Presl

OPTIMAL FERMENTATION CONDITIONS FOR ANTIBIOTIC PRODUCTION BY ENDOPHYTIC Streptomyces cavourensis YBQ59 ISOLATED FROM Cinnamomum cassia Presl

Characterization of Streptomyces Isolates Associated with Estuarine Fish Chanos chanos and Profiling of Their Antibacterial Metabolites-Crude-Extract

Optimization of Culture Medium for the Production and Partial Purification and Characterization of an Antibacterial Activity from Brevibacillus laterosporus Strain EA62

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