Production of antifungal lipopeptide from Bacillus subtilis in submerged fermentation using shake flask and fermentor

Shih, Ing-Lung; Lin, Chih-Yang; Wu, Jane-Yii; Hsieh, Chienyan

doi:10.1007/s11814-009-0237-0

Cited by 27 publications

(14 citation statements)

References 35 publications

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“…They have been used as antifoams, sole carbon sources, auxiliary carbon sources, to provide precursors for antibiotic synthesis and to remove the antibiotic from bacterial access and reduce its suppressive effect on antibiotic production [30]. However, it was considered possible that oil might form a thin film on the surface of the medium, decreasing the oxygen dissolution efficiency, which may influence the production of iturin A [35]. Wu et al [19] verified the negative effects on iturin A production after the addition of either palm or soybean oil.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous hydrolysis with lipase and fermentation of rapeseed cake for iturin A production by Bacillus amyloliquefaciens CX-20

Chen

et al. 2019

BMC Biotechnol

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BackgroundRapeseed cake (RSC), as the intermediate by-product of oil extraction from the seeds of Brassica napus, can be converted into rapeseed meal (RSM) by solvent extraction to remove oil. However, compared with RSM, RSC has been rarely used as a raw material for microbial fermentation, although both RSC and RSM are mainly composed of proteins, carbohydrates and minerals. In this study, we investigated the feasibility of using untreated low-cost RSC as nitrogen source to produce the valuable cyclic lipopeptide antibiotic iturin A using Bacillus amyloliquefaciens CX-20 in submerged fermentation. Especially, the effect of oil in RSC on iturin A production and the possibility of using lipases to improve the iturin A production were analyzed in batch fermentation.ResultsThe maximum production of iturin A was 0.82 g/L at the optimal initial RSC and glucose concentrations of 90 and 60 g/L, respectively. When RSC was substituted with RSM as nitrogen source based on equal protein content, the final concentration of iturin A was improved to 0.95 g/L. The production of iturin A was further increased by the addition of different lipase concentrations from 0.1 to 5 U/mL into the RSC medium for simultaneous hydrolysis and fermentation. At the optimal lipase concentration of 0.5 U/mL, the maximal production of iturin A reached 1.14 g/L, which was 38.15% higher than that without any lipase supplement. Although rapeseed oil and lipase were firstly shown to have negative effects on iturin A production, and the effect would be greater if the concentration of either was increased, their respective negative effects were reduced when used together.ConclusionsAppropriate relative concentrations of lipase and rapeseed oil were demonstrated to support optimal iturin A production. And simultaneous hydrolysis with lipase and fermentation was an effective way to produce iturin A from RSC using B. amyloliquefaciens CX-20.

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Section: Discussionmentioning

confidence: 99%

Simultaneous hydrolysis with lipase and fermentation of rapeseed cake for iturin A production by Bacillus amyloliquefaciens CX-20

Chen

et al. 2019

BMC Biotechnol

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“…al. confirmed that the increase in kLа value favored production of cell biomass, but repressed the production of iturin A [109]. Since the presence of such metabolites is often very desirable in the broth due to their antifungal properties, cultivation should be further optimized with controlling the agitation rate [108].…”

Section: Microorganismmentioning

confidence: 95%

“…Among the three aeration rates studied, the highest sporulation was achieved at the highest aeration rate (V/Vm = 2 L L -1 min -1 ) [67]. The same aeration rate was used for cultivation of B. subtilis and production of Iturin A achieving excellent yields [88]. Another study also emphasized the importance of adequate aeration, claiming that absence of oxygen may result in lysis of sporangia [101].…”

Section: Cultivation Conditionsmentioning

confidence: 99%

Bacillus based microbial formulations: Optimization of the production process

Stojanović

Karabegović

Beškoski

et al. 2019

Hem Ind

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Bacillus sp.-based microbial formulations have found wide application in many fields: from pharmacy and medicine to environmental protection and agriculture due to the ability of this species to produce various metabolites and to form endospores. Recently, these products have gained popularity as biopesticidal and phytostimulatory agents, which are a ?green? alternative to overused agrochemicals. In order to obtain a high-quality and long-lasting product with desired characteristics, it is necessary to optimize the production process at each stage, which implies coordinating the microbial species, the type and the conditions of microbial cultivation along with formulation technologies. This paper provides a concise overview of the most important findings in this area, regarding characteristics of microbial formulations and specific criteria that need to be met when such a product is formulated. It should serve as a beginning point for everyone starting new research, not just in the field of biofertilization and biological control of plant diseases, but generally in the field of biochemical engineering. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 43004]

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“…It is well known that the FFD method can be used to estimate the main effects of factors [17]. It is particularly useful in the initial stages of medium optimization.…”

Section: -1 Fractional Factorial Design (Ffd)mentioning

confidence: 99%

Optimization of medium components for D-ribose production by transketolase-deficient Bacillus subtilis NJT-1507

Fang

Chen

et al. 2010

Korean J. Chem. Eng.

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Statistical experimental designs were used to optimize the composition of culture media for the production of D-ribose by Bacillus subtilis. A fractional factorial design 2 (5-2) was used to determine medium components that significantly affected D-ribose production. The concentrations of glucose and (NH 4 ) 2 SO 4 were the significant factors. Central composite design and response surface methodology were then used to estimate the quadratic response surface and determine the factor levels for maximum production of D-ribose. Finally, the optimal medium composition was obtained (g/L): glucose, 172.75; (NH 4 ) 2 SO 4 , 13.2; yeast powder, 4; corn steep liquor, 8 and MnSO 4 , 0.5. This optimization strategy increased D-ribose production from 73.21 g/L to 88.57 g/L, an increase of 22% compared with the original conditions. The D-ribose production yield to glucose concentration was also enhanced from 0.37 g/g to 0.52 g/g. Confirmatory experiments were also performed to demonstrate the accuracy of the model. Under the optimal medium using ammonia to control pH in a 5 L fermenter, the D-ribose yield was increased to 95.28 g/L after 3 days of cultivation at 37 o C.

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Production of antifungal lipopeptide from Bacillus subtilis in submerged fermentation using shake flask and fermentor

Cited by 27 publications

References 35 publications

Simultaneous hydrolysis with lipase and fermentation of rapeseed cake for iturin A production by Bacillus amyloliquefaciens CX-20

Simultaneous hydrolysis with lipase and fermentation of rapeseed cake for iturin A production by Bacillus amyloliquefaciens CX-20

Bacillus based microbial formulations: Optimization of the production process

Optimization of medium components for D-ribose production by transketolase-deficient Bacillus subtilis NJT-1507

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