Production of Antiserum for Radioimmunoassay of Arginine Vasotocin

Goto, Kazufumi; 和文, 後藤; 耕作, 田中; Tanaka, Kousaku; 俊弘, 寺尾; Toshihiro, Terao

doi:10.2508/chikusan.57.614

Cited by 6 publications

(6 citation statements)

References 1 publication

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“…The amount of AVT was measured by RIA using the method of Goto et al (1986). The sensitivity of the assay as reflected by the dose at which 90% of labeled AVT was bound, which was 8.8 + .6 pg/mL (x ± SEM), as 1 determined by eight assays.…”

Section: Methodsmentioning

confidence: 99%

Effect of Estradiol-17β and Progesterone on Arginine Vasotocin in Nonlaying Hens

et al. 1994

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Section: Methodsmentioning

confidence: 99%

Effect of Estradiol-17β and Progesterone on Arginine Vasotocin in Nonlaying Hens

et al. 1994

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“…Fifteen tissues of neurohypophysis were pooled as 1 sample for the binding assay to [ 3 H]PGF 2α . Blood was collected from the wing vein in 6 hens, and serum concentrations of AVT were measured by a routine RIA (Goto et al, 1986;Takahashi et al, 1994a,b).…”

Section: Birds and Tissuesmentioning

confidence: 99%

Prostaglandin F2α receptor in the neurohypophysis of hens

Takahashi

Kawashima

2009

Poultry Science

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To elucidate whether the receptor for prostaglandin (PG) F(2alpha), one of PG, exists in the neurohypophysis in hens and whether the binding of receptor changes with relation to oviposition, the PGF(2alpha) binding component in the membrane fraction of the neurohypophysis of laying hens was analyzed by radioligand binding assay using [5,6,8,9,11,12,14,15(n)-(3)H]PGF(2alpha). The binding component had characteristics of a receptor such as binding specificity, high affinity, and limited capacity for PGF(2alpha). Scatchard analysis indicated that the binding site was of a single class. The binding capacity of the receptor was smaller in laying hens than in nonlaying hens, whereas the binding affinity was not significantly different between these hens. When non-laying hens received an i.m. injection of estradiol-17beta or progesterone (0.5 mg/hen), the specific binding of the PGF(2alpha) receptor in the neurohypophysis was decreased. In laying hens, the specific binding decreased and the blood arginine vasotocin (AVT) concentration increased just after oviposition but did not change during a 24-h day in nonlaying hens. An i.v. injection of PGF(2alpha) (2 microg/hen) induced oviposition and caused an increase in the blood AVT concentration with a decrease in the specific binding of PGF(2alpha) receptor. The present study suggests a possibility that PGF(2alpha) may directly cause the AVT release from the neurohypophysis at oviposition time in hens.

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“…The AVT was extracted from 1 mL of the serum using an octadecasilyl-silica cartridge (Sep Pak C 18 ; Waters Corp., Milford, MA) as reported earlier (Takahashi et al, 1994b), and AVT concentrations were measured by a routine RIA (Goto et al, 1986b).…”

Section: Birds and Tissuesmentioning

confidence: 99%

Changes in angiotensin II receptor bindings in the hen neurohypophysis before and after oviposition

et al. 2011

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The present study was performed to elucidate whether the angiotensin II (ANG II) receptor exists in the plasma membrane fraction of the neurohypophysis in hens, to estimate the time of action of ANG II on the neurohypophysis before and after oviposition, and to examine relationships between the action of ANG II on the neurohypophysis and those of estrogen and prostaglandin F(2α) (PGF(2α)) in relation to arginine vasotocin (AVT) release. The specific binding had a binding specificity to chicken ANG II (cANG II), reversibility, and saturation in the [(125)I]cANG II binding assay. Scatchard analysis revealed that the binding sites are of a single class. The equilibrium dissociation constant (K(d)) obtained by kinetic analysis and Scatchard analysis suggested a high affinity, and the maximum binding capacity (B(max)) obtained by Scatchard analysis suggested a limited capacity. These results suggest that an ANG II receptor exists in the neurohypophysis of hens. The K(d) and the B(max) value was significantly smaller in laying hens than in nonlaying hens, which suggests that bindings of the cANG II receptor change, depending on the difference in laying condition. Values of the K(d) and the B(max) decreased approximately 15 min before oviposition in laying hens, and decreased 1 h after an intramuscular injection of estradiol-17β and 5 min after an intravenous injection of cANG II in nonlaying hens. The amount of specific binding of PGF(2α) receptor in the neurohypophysis also decreased and AVT concentration in blood increased after the cANG II injection. It seems likely that the action of cANG II in the neurohypophysis increases due to the effect of estrogen approximately 15 min before oviposition, and the cANG II action stimulates AVT release through the increase in the PGF(2α) action in this tissue.

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Production of Antiserum for Radioimmunoassay of Arginine Vasotocin

Cited by 6 publications

References 1 publication

Effect of Estradiol-17β and Progesterone on Arginine Vasotocin in Nonlaying Hens

Effect of Estradiol-17β and Progesterone on Arginine Vasotocin in Nonlaying Hens

Prostaglandin F2α receptor in the neurohypophysis of hens

Changes in angiotensin II receptor bindings in the hen neurohypophysis before and after oviposition

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