Production of auxins, gibberellins and cytokinins by Azotobacter vinelandii ATCC 12837 in chemically-defined media and dialysed soil media

González‐López, Jesús; Salmerón, V.; Martı́nez-Toledo, M.V.; Ballesteros, F; Ramos‐Cormenzana, A.

doi:10.1016/0038-0717(86)90115-x

Cited by 54 publications

(21 citation statements)

References 10 publications

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“…The increase in nodule number/plant due to their combined effect reached 10, 95, 86 and 55%, respectively, for Kawmy-1, VC-4, VC-9 and King over the corresponding biotreatment with Bradyrhizobium. These increases could be attributed to the synergistic interaction between the microorganisms and the plant growth promoting substances produced by Azotobacter vinelandii (Gonzalez-Lopez et al, 1986) that may enhance the penetration of Bradyrhizobium into mungbean roots.…”

Section: Discussionmentioning

confidence: 99%

Response of a Local and Some Exotic Mungbean Varieties to Bio- And Mineral Fertilization

El-Kramany

Bahr

Gomaa

2001

Acta Agronomica Hungarica

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A pot trial was carried out in the greenhouse of the National Research Centre during the summer season of 1999 to investigate the combined impact of Bradyrhizobium sp. (vigna) and Azotobacter vinelandii in the presence of various doses of chemical fertilizers, i.e. 25%, 50% and 100% of the recommended dose of NPK, on nodulation, growth parameters, seed yield and its components, and seed contents of protein, phosphorus and potassium of a local (Kawmy-1) and three exotic (VC-4, VC-9 and King) varieties of mungbean. The results indicated that there were no significant differences between the different varieties for nodule number per plant, while significant variations were obtained between both varieties and biofertilization treatments.The plants of the Kawmy-1 variety gave the shortest period of growth, the highest number of pods per plant and the highest values of harvest index and seed protein content. The King variety had the longest period of growth and the highest values for number of branches, seed yield, biological yield, seed index and seed phosphorus content. The inclusion of Azotobacter vinelandii significantly augmented various tested parameters, with the exception of seed yield, biological yield and potassium content, in comparison with Bradyrhizobium. Insignificant differences were found owing to the interaction between varieties, various levels of mineral fertilizers and types of biofertilizers with the exception of harvest index and seed phosphorus content.

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Section: Discussionmentioning

confidence: 99%

Response of a Local and Some Exotic Mungbean Varieties to Bio- And Mineral Fertilization

El-Kramany

Bahr

Gomaa

2001

Acta Agronomica Hungarica

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“…Similar findings have been recorded by Verma et al (2010) in Rhizobium, Pseudomonas and Azotobacter. In Azospirillum and Pseudomonas, production of cytokinins, auxins and gibberellins in the chemically defined media has also been widely studied and reported (Gonzalez-Lopez et al, 1986). Among Chickpea isolates, sixteen bacterial isolates were positive in phosphate solubilizing ability while only one isolate was negative.…”

Section: Discussionmentioning

confidence: 99%

MOLECULAR AND BIOCHEMICAL CHARACTERIZATION OF RHIZOBIA FROM CHICKPEA (Cicer arietinum)

Zafar¹

2017

PAKJAS

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“…Root attachment and colonization constitute an important stage, and little is known about their mechanisms. A. vinelandii has been known to be a good plant growth-promoting bacterium (2,4). In this study, the response of A. vinelandii to association with the roots of O. sativa was examined under different growth medium conditions.…”

Section: Discussionmentioning

confidence: 99%

“…For the comparative proteomic analyses involving plant root-microbe interaction studies, a modified formulation based on the Murashige-Skoog basal medium with vitamins was used (15). This modified medium (designated AMSϩN) consisted of NH 4 For generation of a disrupted mutant using homologous recombination, the kanamycin gene from plasmid pJRD215 was used (16). Plasmid pDM4 was used to assemble the DNA construct containing the disrupted allele (17).…”

Section: Methodsmentioning

confidence: 99%

Hypothetical Protein Avin_16040 as the S-Layer Protein of Azotobacter vinelandii and Its Involvement in Plant Root Surface Attachment

Liew

Jong

Najimudin

2015

Appl Environ Microbiol

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A proteomic analysis of a soil-dwelling, plant growth-promoting Azotobacter vinelandii strain showed the presence of a protein encoded by the hypothetical Avin_16040 gene when the bacterial cells were attached to the Oryza sativa root surface. An Avin_16040 deletion mutant demonstrated reduced cellular adherence to the root surface, surface hydrophobicity, and biofilm formation compared to those of the wild type. By atomic force microscopy (AFM) analysis of the cell surface topography, the deletion mutant displayed a cell surface architectural pattern that was different from that of the wild type. Escherichia coli transformed with the wild-type Avin_16040 gene displayed on its cell surface organized motifs which looked like the S-layer monomers of A. vinelandii. The recombinant E. coli also demonstrated enhanced adhesion to the root surface. Azotobacter vinelandii is a Gram-negative free-living and obligate aerobic soil bacterium. It is well known to be a plant growth-promoting bacterium capable of fixing nitrogen and forming desiccation-resistant cysts under unfavorable growth condition (1, 2). The former activity requires it to house several oxygen-sensitive mechanisms while being an obligate aerobic bacterium (3). A. vinelandii also has characteristics such as production of plant growth hormones and antibiotics (4) as well as industrially important substances such as extracellular polysaccharide (EPS) alginate, poly-␤-hydroxybutyrate (PHB), and siderophore compounds (5).Many diverse genera of nitrogen-fixing bacteria are present in the plant rhizosphere. The effectiveness of their plant growthpromoting activity depends upon the establishment of their cells in the rhizosphere. This interaction depends upon many factors, one of them being plant exudates. As a diazotroph, A. vinelandii provides fixed nitrogen to the plant while acquiring sugars and other nutrients that leak from the roots (6).The complete A. vinelandii genome (GenBank accession number NC_012560) has explained many biochemical pathways and structures of the bacterium (7). It has also revealed hypothetical genes with unannotated functions. The advances in proteomic technology have led to new understanding of and insights into many important proteins and their related mechanisms.Studies have shown that plant-microbe communication is a two-way interaction involving various signal molecules that cause metabolic changes in both organisms (8-10). Nevertheless, there is limited information on the plant-bacterium interaction, especially with the roots and the rhizosphere. In this study, a proteomic approach was successfully used to study the interaction between a root-associated bacterium and rice plant in the rhizosphere (11-13).In this study, a differential proteomic analysis of A. vinelandii ATCC 12837 in response to different conditions and at different locations within the Oryza sativa MR 219 rhizosphere was performed. By two-dimensional gel electrophoresis (2DE) followed by tandem mass spectrometry (MS/MS) analyses, several known and hypothetical p...

show abstract

Production of auxins, gibberellins and cytokinins by Azotobacter vinelandii ATCC 12837 in chemically-defined media and dialysed soil media

Cited by 54 publications

References 10 publications

Response of a Local and Some Exotic Mungbean Varieties to Bio- And Mineral Fertilization

Response of a Local and Some Exotic Mungbean Varieties to Bio- And Mineral Fertilization

MOLECULAR AND BIOCHEMICAL CHARACTERIZATION OF RHIZOBIA FROM CHICKPEA (Cicer arietinum)

Hypothetical Protein Avin_16040 as the S-Layer Protein of Azotobacter vinelandii and Its Involvement in Plant Root Surface Attachment

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